Strain identifier
BacDive ID: 24730
Type strain: 
Species: Actinopolyspora mzabensis
Strain Designation: H55
Strain history: <- N. Sabaou, LPBVB, Kouba, Algeria; H55
NCBI tax ID(s): 995066 (species)
version 9.1 (current version)
General
@ref: 20252
BacDive-ID: 24730
DSM-Number: 45460
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, rod-shaped
description: Actinopolyspora mzabensis H55 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from arid soil from a palm grove.
NCBI tax id
- NCBI tax id: 995066
- Matching level: species
strain history
- @ref: 20252
- history: <- N. Sabaou, LPBVB, Kouba, Algeria; H55
doi: 10.13145/bacdive24730.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Actinopolyspora
- species: Actinopolyspora mzabensis
- full scientific name: Actinopolyspora mzabensis Meklat et al. 2013
@ref: 20252
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Actinopolysporaceae
genus: Actinopolyspora
species: Actinopolyspora mzabensis
full scientific name: Actinopolyspora mzabensis Meklat et al. 2013 emend. Nouioui et al. 2018
strain designation: H55
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 30881 | positive | 1.45 µm | 0.55 µm | rod-shaped | no | |
| 69480 | positive | 91.293 | ||||
| 69480 | no | 91 |
colony morphology
- @ref: 62916
- incubation period: 18-20 days
pigmentation
- @ref: 30881
- production: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 20252 | GYM STREPTOMYCES MEDIUM 10% NACL (DSMZ Medium 1159) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1159.pdf | |
| 20252 | CM-MEDIUM (DSMZ Medium 1504) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1504.pdf | |
| 20252 | NUTRIMENT AGAR WITH 150G/L NACL (DSMZ Medium 1258) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1258.pdf | |
| 20252 | CM + YE MEDIUM (DSMZ Medium 549) | yes | https://mediadive.dsmz.de/medium/549 | Name: CM + YE MEDIUM (DSMZ Medium 549) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Casamino acids 7.5 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l FeSO4 x 7 H2O 0.01 g/l HCl 3.646e-05 g/l Distilled water |
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 20252 | positive | growth | 28 |
| 30881 | positive | growth | 20-40 |
| 30881 | positive | optimum | 30 |
| 62916 | positive | growth | 20-30 |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 30881 | positive | growth | 05-08 |
| 30881 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 30881 | aerobe |
| 62916 | aerobe |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 30881 | yes | |
| 69481 | yes | 99 |
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 30881 | NaCl | positive | growth | 07-32 %(w/v) |
| 30881 | NaCl | positive | optimum | 10-28 % |
observation
- @ref: 30881
- observation: aggregates in chains
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30881 | 15963 | ribitol | + | carbon source |
| 30881 | 16449 | alanine | + | carbon source |
| 30881 | 22599 | arabinose | + | carbon source |
| 30881 | 17057 | cellobiose | + | carbon source |
| 30881 | 16947 | citrate | + | carbon source |
| 30881 | 28757 | fructose | + | carbon source |
| 30881 | 28260 | galactose | + | carbon source |
| 30881 | 17234 | glucose | + | carbon source |
| 30881 | 17754 | glycerol | + | carbon source |
| 30881 | 17716 | lactose | + | carbon source |
| 30881 | 17306 | maltose | + | carbon source |
| 30881 | 37684 | mannose | + | carbon source |
| 30881 | 26271 | proline | + | carbon source |
| 30881 | 26546 | rhamnose | + | carbon source |
| 30881 | 33942 | ribose | + | carbon source |
| 30881 | 30911 | sorbitol | + | carbon source |
| 30881 | 17992 | sucrose | + | carbon source |
| 30881 | 27082 | trehalose | + | carbon source |
| 30881 | 18222 | xylose | + | carbon source |
| 30881 | 17632 | nitrate | + | reduction |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | sampling date |
|---|---|---|---|---|---|---|---|---|
| 20252 | arid soil from a palm grove | Sahara, Mzab region, Ghardaia, Ntissa palm grove (Béni-isguen), 32° 29' N 3° 41' E | Algeria | DZA | Africa | 32.4833 | 3.6833 | |
| 62916 | Soil,Sahara desert | Chardaia,Mzab region | Algeria | DZA | Africa | 2006 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Forest |
| #Environmental | #Terrestrial | #Soil |
| #Climate | #Hot | #Arid |
taxonmaps
- @ref: 69479
- File name: preview.99_162093.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_495;96_2848;97_3502;98_36039;99_162093&stattab=map
- Last taxonomy: Actinopolyspora mzabensis
- 16S sequence: HQ918202
- Sequence Identity:
- Total samples: 1
- aquatic counts: 1
Safety information
risk assessment
- @ref: 20252
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20252
- description: Actinopolyspora mzabensis strain H55 16S ribosomal RNA gene, partial sequence
- accession: HQ918202
- length: 1530
- database: nuccore
- NCBI tax ID: 995066
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Actinopolyspora mzabensis DSM 45460 | GCA_900101095 | scaffold | ncbi | 995066 |
| 66792 | Actinopolyspora mzabensis strain DSM 45460 | 995066.3 | wgs | patric | 995066 |
| 66792 | Actinopolyspora mzabensis DSM 45460 | 2619619027 | draft | img | 995066 |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 99 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 91.293 | yes |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 95.246 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 87.765 | yes |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 85.026 | yes |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 96.05 | no |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | no | 91 | yes |
External links
@ref: 20252
culture collection no.: DSM 45460, CCUG 62965
straininfo link
- @ref: 88558
- straininfo: 402809
literature
- topic: Phylogeny
- Pubmed-ID: 23667146
- title: Actinopolyspora mzabensis sp. nov., a halophilic actinomycete isolated from an Algerian Saharan soil.
- authors: Meklat A, Bouras N, Zitouni A, Mathieu F, Lebrihi A, Schumann P, Sproer C, Klenk HP, Sabaou N
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.046649-0
- year: 2013
- mesh: Actinomycetales/*classification/genetics/isolation & purification, Arabinose/analysis, Bacterial Typing Techniques, Cell Wall/chemistry, DNA, Bacterial/genetics, *Desert Climate, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Galactose/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phosphatidylcholines/analysis, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analysis
- topic2: Genetics
Reference
| @id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 20252 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-45460 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45460) | |||
| 30881 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 27211 | 28776041 | |
| 62916 | Curators of the CCUG | https://www.ccug.se/strain?id=62965 | Culture Collection University of Gothenburg (CCUG) (CCUG 62965) | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |||
| 88558 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID402809.1 |