General

@ref: 20644

BacDive-ID: 24262

DSM-Number: 26121

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Massilia umbonata LP01 is a mesophilic bacterium that was isolated from mixture of soil and sewage sludge compost.

NCBI tax id

• NCBI tax id: 864828
• Matching level: species

strain history

• @ref: 20644
• history: <- C. Pozo-Llorente, University of Granada, Institute of water research; LP01 <- M. Diaz

doi: 10.13145/bacdive24262.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/pseudomonadota
• domain: Bacteria
• phylum: Pseudomonadota
• class: Betaproteobacteria
• order: Burkholderiales
• family: Oxalobacteraceae
• genus: Massilia
• species: Massilia umbonata
• full scientific name: Massilia umbonata Rodríguez-Díaz et al. 2014

• synonyms

  • @ref: 20215
  • synonym: Pseudoduganella umbonata

@ref: 20644

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Oxalobacteraceae

genus: Massilia

species: Massilia umbonata

full scientific name: Massilia umbonata Rodríguez-Díaz et al. 2014

strain designation: LP01

type strain: yes

Morphology

colony morphology

• @ref: 20644
• incubation period: 2-3 days

Culture and growth conditions

culture medium

• @ref: 20644
• name: R2A MEDIUM (DSMZ Medium 830)
• growth: yes
• link: https://mediadive.dsmz.de/medium/830
• composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

• @ref: 20644
• growth: positive
• type: growth
• temperature: 30
• range: mesophilic

Physiology and metabolism

metabolite utilization

metabolite production

• @ref: 68369
• Chebi-ID: 35581
• metabolite: indole
• production: no

metabolite tests

• @ref: 68369
• Chebi-ID: 35581
• metabolite: indole
• indole test: -

enzymes

API 20NE

Isolation, sampling and environmental information

isolation

• @ref: 20644
• sample type: mixture of soil and sewage sludge compost
• geographic location: province of Jaén, nearby Guadalimar (soil) and Granada
• country: Spain
• origin.country: ESP
• continent: Europe

isolation source categories

Safety information

risk assessment

• @ref: 20644
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 20644
• description: Massilia umbonata strain LP01 16S ribosomal RNA gene, partial sequence
• accession: HM053474
• length: 1434
• database: ena
• NCBI tax ID: 864828

Genome sequences

GC content

• @ref: 20644
• GC-content: 66.0
• method: high performance liquid chromatography (HPLC)

External links

@ref: 20644

culture collection no.: DSM 26121, CECT 7753

straininfo link

• @ref: 88204
• straininfo: 391806

literature

• topic: Phylogeny
• Pubmed-ID: 24030691
• title: Massilia umbonata sp. nov., able to accumulate poly-beta-hydroxybutyrate, isolated from a sewage sludge compost-soil microcosm.
• authors: Rodriguez-Diaz M, Cerrone F, Sanchez-Peinado M, SantaCruz-Calvo L, Pozo C, Lopez JG
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijs.0.049874-0
• year: 2013
• mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hydroxybutyrates/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification/metabolism, *Phylogeny, Polyesters/*metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Soil, *Soil Microbiology, Spain, Ubiquinone/chemistry
• topic2: Metabolism

Reference