Strain identifier

BacDive ID: 24255

Type strain: Yes

Species: Flindersiella endophytica

Strain history: DSM 45355 <-- C. M. M. Franco; Flinders Univ., Australia; EUM 378 <-- O. Kaewkla.

NCBI tax ID(s): 648751 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 21222

BacDive-ID: 24255

DSM-Number: 45355

keywords: 16S sequence, Bacteria, mesophilic

description: Flindersiella endophytica DSM 45355 is a mesophilic bacterium that builds an aerial mycelium and was isolated from surface-sterilized root of Eucalyptus microcarpa .

NCBI tax id

  • NCBI tax id: 648751
  • Matching level: species

strain history

@refhistory
21222<- C. Franco, Flinders Univ., Bedford Park, South Australia; EUM 378 <- O. Kaewkla
67770DSM 45355 <-- C. M. M. Franco; Flinders Univ., Australia; EUM 378 <-- O. Kaewkla.

doi: 10.13145/bacdive24255.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Actinopolymorphaceae
  • genus: Flindersiella
  • species: Flindersiella endophytica
  • full scientific name: Flindersiella endophytica Kaewkla and Franco 2011

@ref: 21222

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinopolymorphaceae

genus: Flindersiella

species: Flindersiella endophytica

full scientific name: Flindersiella endophytica Kaewkla and Franco 2011

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
69386Ivory (1014)ISP 6
69386Khaki grey (7008)suter with tyrosine
69386Light ivory (1015)ISP 2
69386Light ivory (1015)ISP 3
69386Light ivory (1015)ISP 4
69386Light ivory (1015)ISP 5
69386Light ivory (1015)ISP 7
69386Reed green (6013), olive green (6003)suter without tyrosine

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69386noAerial myceliumISP 2
69386noAerial myceliumISP 3
69386noAerial myceliumISP 4
69386yesAerial myceliumISP 5Signal white (9003)
69386noAerial myceliumISP 6
69386yesAerial myceliumISP 7Signal white (9003)
69386noAerial myceliumsuter with tyrosine
69386noAerial myceliumsuter without tyrosine

pigmentation

@refproductionname
69386noMelanin
69386nosoluble pigment

multimedia

@refmultimedia contentcaptionintellectual property rights
21222https://www.dsmz.de/microorganisms/photos/DSM_45355.jpgMedium 65 28°C© Leibniz-Institut DSMZ
69386DSM_45355_image3.jpegPlates (535, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69386DSM_45355_image4.jpegPlates (535, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
21222GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
21222TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
21222GPHF-MEDIUM (DSMZ Medium 553)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium553.pdf
21222ISP2 MEDIUM (DSMZ Medium 987)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium987.pdf

culture temp

@refgrowthtypetemperaturerange
21222positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

halophily

  • @ref: 69386
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-2.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-10(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6938622599arabinose+growth
6938662968cellulose-growth
6938628757fructose+growth
6938617234glucose+growth
6938617268inositol+growth
6938637684mannose+growth
6938616634raffinose+growth
6938626546rhamnose+growth
6938617992sucrose+growth
6938618222xylose+growth
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382alpha-mannosidase+3.2.1.24

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69386-----++---+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69386+/-+++/-++/--+/-+/-+/-+/-++-+++/-++/-

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
21222surface-sterilized root of Eucalyptus microcarpa (Grey box tree)Eucalyptus microcarpaAdelaide, Flinders University campusAustraliaAUSAustralia and Oceania
67770Surface-sterilized root tissue of a Eucalyptus microcarpa tree on the campus of Flinders Univ.Eucalyptus microcarpaAdelaide, South AustraliaAustraliaAUSAustralia and Oceania

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Tree
#Host Body-Site#Plant#Root (Rhizome)
#Host Body-Site#Plant#Sterilized plant part

taxonmaps

  • @ref: 69479
  • File name: preview.99_122048.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_779;96_47965;97_61672;98_82112;99_122048&stattab=map
  • Last taxonomy: Flindersiella endophytica subclade
  • 16S sequence: FJ805430
  • Sequence Identity:
  • Total samples: 7243
  • soil counts: 5720
  • aquatic counts: 439
  • animal counts: 483
  • plant counts: 601

Safety information

risk assessment

  • @ref: 21222
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21222
  • description: Flindersiella endophytica strain EUM 378 16S ribosomal RNA gene, partial sequence
  • accession: FJ805430
  • length: 1401
  • database: ena
  • NCBI tax ID: 648751

GC content

  • @ref: 21222
  • GC-content: 68.8
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 21222

culture collection no.: DSM 45355, ACM 5289, JCM 31030, EUM 378

straininfo link

  • @ref: 88197
  • straininfo: 405811

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny20889769Flindersiella endophytica gen. nov., sp. nov., an endophytic actinobacterium isolated from the root of Grey Box, an endemic eucalyptus tree.Kaewkla O, Franco CMMInt J Syst Evol Microbiol10.1099/ijs.0.026757-02010Actinomycetales/*classification/genetics/*isolation & purification/physiology, Aerobiosis, Bacterial Typing Techniques, Cell Wall/chemistry, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Eucalyptus/*microbiology, Glucose/analysis, Molecular Sequence Data, Phylogeny, Plant Roots/microbiology, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Rhamnose/analysis, Ribose/analysis, Sequence Analysis, DNAGenetics
Phylogeny26297147Tenggerimyces mesophilus gen. nov., sp. nov., a member of the family Nocardioidaceae.Sun HM, Zhang T, Wei YZ, Liu HY, Yu LY, Zhang YQInt J Syst Evol Microbiol10.1099/ijsem.0.0004212015Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistryGenetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21222Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-45355Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45355)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69386Wink, J.Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweighttps://cdn.dsmz.de/wink/DSM%2045355.pdf
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88197Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID405811.1