Name: Flindersiella endophytica DSM 45355
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24255

Type strain:

Species: Flindersiella endophytica

Culture col. no.: DSM 45355, ACM 5289, JCM 31030, EUM 378

Strain history: DSM 45355 <-- C. M. M. Franco; Flinders Univ., Australia; EUM 378 <-- O. Kaewkla.

NCBI tax ID(s): 648751 (species)

SI-ID 405811

DSM 45355

Section

Flindersiella endophytica DSM 45355 is a mesophilic bacterium that builds an aerial mycelium and was isolated from surface-sterilized root of Eucalyptus microcarpa .

mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Actinopolymorphaceae*
	Genus	*Flindersiella*
	Species	**Flindersiella endophytica**
	Full Scientific Name (LPSN)	Flindersiella endophytica Kaewkla and Franco 2011

Information on morphological and physiological properties Morphology

[Ref.: #69386]	Colony color	Ivory (1014)
[Ref.: #69386]	Cultivation medium used	ISP 6

[Ref.: #69386]	Colony color	Khaki grey (7008)
[Ref.: #69386]	Cultivation medium used	suter with tyrosine

[Ref.: #69386]	Colony color	Light ivory (1015)
[Ref.: #69386]	Cultivation medium used	ISP 2

[Ref.: #69386]	Colony color	Light ivory (1015)
[Ref.: #69386]	Cultivation medium used	ISP 3

[Ref.: #69386]	Colony color	Light ivory (1015)
[Ref.: #69386]	Cultivation medium used	ISP 4

[Ref.: #69386]	Colony color	Light ivory (1015)
[Ref.: #69386]	Cultivation medium used	ISP 5

[Ref.: #69386]	Colony color	Light ivory (1015)
[Ref.: #69386]	Cultivation medium used	ISP 7

[Ref.: #69386]	Colony color	Reed green (6013), olive green (6003)
[Ref.: #69386]	Cultivation medium used	suter without tyrosine

[Ref.: #69386]	Pigment production	no
[Ref.: #69386]	Pigment name	Melanin
[Ref.: #69386]	Pigment production	no
[Ref.: #69386]	Pigment name	soluble pigment

[Ref.: #69386]	Multicellular complex forming ability	no
[Ref.: #69386]	Multicellular complex name	Aerial mycelium
[Ref.: #69386]	Medium Name (multicellularity)	ISP 2

[Ref.: #69386]	Multicellular complex forming ability	no
[Ref.: #69386]	Multicellular complex name	Aerial mycelium
[Ref.: #69386]	Medium Name (multicellularity)	ISP 3

[Ref.: #69386]	Multicellular complex forming ability	no
[Ref.: #69386]	Multicellular complex name	Aerial mycelium
[Ref.: #69386]	Medium Name (multicellularity)	ISP 4

[Ref.: #69386]	Multicellular complex forming ability	yes
[Ref.: #69386]	Multicellular complex name	Aerial mycelium
[Ref.: #69386]	Multicellular complex color	Signal white (9003)
[Ref.: #69386]	Medium Name (multicellularity)	ISP 5

[Ref.: #69386]	Multicellular complex forming ability	no
[Ref.: #69386]	Multicellular complex name	Aerial mycelium
[Ref.: #69386]	Medium Name (multicellularity)	ISP 6

[Ref.: #69386]	Multicellular complex forming ability	yes
[Ref.: #69386]	Multicellular complex name	Aerial mycelium
[Ref.: #69386]	Multicellular complex color	Signal white (9003)
[Ref.: #69386]	Medium Name (multicellularity)	ISP 7

[Ref.: #69386]	Multicellular complex forming ability	no
[Ref.: #69386]	Multicellular complex name	Aerial mycelium
[Ref.: #69386]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69386]	Multicellular complex forming ability	no
[Ref.: #69386]	Multicellular complex name	Aerial mycelium
[Ref.: #69386]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #21222]

Caption:

Medium 65 28°C

[Ref.: #21222]

Intellectual property rights:

[Ref.: #69386]

Caption:

Plates (535, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69386]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69386]

Caption:

Plates (535, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69386]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21222]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #21222]

Culture medium growth

[Ref.: #21222]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21222]

Culture medium composition

expand / minimize

[Ref.: #21222]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #21222]

Culture medium growth

[Ref.: #21222]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21222]

Culture medium composition

expand / minimize

[Ref.: #21222]

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

[Ref.: #21222]

Culture medium growth

[Ref.: #21222]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #21222]

Culture medium

ISP2 MEDIUM (DSMZ Medium 987)

[Ref.: #21222]

Culture medium growth

[Ref.: #21222]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #21222]			growth	28 °C
[Ref.: #67770]			growth	28 °C

[Ref.: #21222]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69386]		NaCl		growth	0-2.5	%

[Ref.: #67770]

Observation

quinones: MK-10(H₆)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69386]	arabinose ChEBI	+	growth
[Ref.: #69386]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69386]	fructose ChEBI	+	growth
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69386]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69386]	inositol ChEBI	+	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69386]	mannose ChEBI	+	growth
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69386]	raffinose ChEBI	+	growth
[Ref.: #69386]	rhamnose ChEBI	+	growth
[Ref.: #69386]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69386]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68379]	alkaline phosphatase	-	3.1.3.1	* from API Coryne
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	+	3.2.1.23	* from API Coryne, API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69386]	3879	-	-	-	-	-	+	+	-	-	-	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69386]	6063	not determinedn.d.	+/-	+	+	+/-	+	+/-	-	+/-	+/-	+/-	+/-	+	+	-	+	+	+/-	+	+/-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21222]	Sample type/isolated from	surface-sterilized root of Eucalyptus microcarpa (Grey box tree)
[Ref.: #21222]	Host species	Eucalyptus microcarpa
[Ref.: #21222]	Geographic location (country and/or sea, region)	Adelaide, Flinders University campus
[Ref.: #21222]	Country	Australia
[Ref.: #21222]	Country ISO 3 Code	AUS
[Ref.: #21222]	Continent	Australia and Oceania

[Ref.: #67770]	Sample type/isolated from	Surface-sterilized root tissue of a Eucalyptus microcarpa tree on the campus of Flinders Univ.
[Ref.: #67770]	Host species	Eucalyptus microcarpa
[Ref.: #67770]	Geographic location (country and/or sea, region)	Adelaide, South Australia
[Ref.: #67770]	Country	Australia
[Ref.: #67770]	Country ISO 3 Code	AUS
[Ref.: #67770]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Tree
#Host Body-Site	#Plant	#Root (Rhizome)
#Host Body-Site	#Plant	#Sterilized plant part

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FJ805430 (>99% sequence identity) for Flindersiella endophytica subclade from Microbeatlas

Aquatic Samples	439
Soil Samples	5720
Animal Samples	483
Plant Samples	601
Total Samples	7243

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21222]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21222]	Flindersiella endophytica strain EUM 378 16S ribosomal RNA gene, partial sequence	FJ805430	1401	ENA	648751 tax ID

[Ref.: #21222]

GC-content

68.8 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #21222]

Culture collection no.

DSM 45355, ACM 5289, JCM 31030, EUM 378

[Ref.: #88197]

SI-ID 405811

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Tenggerimyces mesophilus gen. nov., sp. nov., a member of the family Nocardioidaceae.	Sun HM, Zhang T, Wei YZ, Liu HY, Yu LY, Zhang YQ	Int J Syst Evol Microbiol	10.1099/ijsem.0.000421	2015	*
Phylogeny	Flindersiella endophytica gen. nov., sp. nov., an endophytic actinobacterium isolated from the root of Grey Box, an endemic eucalyptus tree.	Kaewkla O, Franco CMM	Int J Syst Evol Microbiol	10.1099/ijs.0.026757-0	2010	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21222

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45355

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69386

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#88197

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405811.1 )

* These data were automatically processed and therefore are not curated

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