General

@ref: 21021

BacDive-ID: 24168

DSM-Number: 26047

keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic

description: Methanosarcina spelaei MC-15 is an anaerobe, mesophilic archaeon that was isolated from floating biofilm of a sulphurous subsurface lake.

NCBI tax id

• NCBI tax id: 1036679
• Matching level: species

strain history

doi: 10.13145/bacdive24168.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Methanomicrobia
• order: Methanosarcinales
• family: Methanosarcinaceae
• genus: Methanosarcina
• species: Methanosarcina spelaei
• full scientific name: Methanosarcina spelaei Ganzert et al. 2014

@ref: 21021

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina spelaei

full scientific name: Methanosarcina spelaei Ganzert et al. 2014

strain designation: MC-15

type strain: yes

Morphology

colony morphology

• @ref: 21021
• incubation period: 2-3 days

multimedia

• @ref: 21021
• multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_26047.jpg
• caption: Medium 120a, 30°C
• intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

• @ref: 21021
• name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a)
• growth: yes
• link: https://mediadive.dsmz.de/medium/120a
• composition: Name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a) Composition: Methanol 15.4688 g/l NaCl 2.19727 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NaHCO3 0.830078 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Thiamine HCl 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water

culture temp

Physiology and metabolism

oxygen tolerance

• @ref: 21021
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

isolation source categories

Safety information

risk assessment

• @ref: 21021
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 21021
• description: Methanosarcina spelaei strain MC-15 16S ribosomal RNA gene, partial sequence
• accession: JF812257
• length: 1346
• database: ena
• NCBI tax ID: 1036679

Genome sequences

GC content

External links

@ref: 21021

culture collection no.: DSM 26047, JCM 18469

straininfo link

• @ref: 88123
• straininfo: 397321

literature

• topic: Phylogeny
• Pubmed-ID: 25052394
• title: Methanosarcina spelaei sp. nov., a methanogenic archaeon isolated from a floating biofilm of a subsurface sulphurous lake.
• authors: Ganzert L, Schirmack J, Alawi M, Mangelsdorf K, Sand W, Hillebrand-Voiculescu A, Wagner D
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijs.0.064956-0
• year: 2014
• mesh: Base Composition, *Biofilms, Caves/microbiology, DNA, Archaeal/genetics, DNA, Bacterial/genetics, Lakes/microbiology, Lipids/chemistry, Methanosarcina/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Romania, Sequence Analysis, DNA, Sulfur, *Water Microbiology
• topic2: Genetics

Reference