Methanosarcina spelaei (DSM 26047, JCM 18469, MC-15)

Cell morphology

Colony morphology

Multimedia

• @ref:	21021
  multimedia content:	DSM_26047.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_26047.jpg
  caption:	Medium 120a, 30°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
21021	METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a)		Medium recipe at MediaDive	Name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a) Composition: Methanol 15.4688 g/l NaCl 2.19727 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NaHCO3 0.830078 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Thiamine HCl 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water

Culture temp

Oxygen tolerance

Spore formation

Isolation source categories

Isolation

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID
67770	ASM228723v1 assembly for Methanosarcina spelaei MC-15	contig	GCA_002287235	1036679.3	2791355116	1036679

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21021	Methanosarcina spelaei strain MC-15 16S ribosomal RNA gene, partial sequence	JF812257	1346		1036679
124043	Methanosarcina spelaei gene for 16S ribosomal RNA, partial sequence, strain: DSM 26047.	LC006853	1409		1036679

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Genomic analysis of methanogenic archaea reveals a shift towards energy conservation.	Gilmore SP, Henske JK, Sexton JA, Solomon KV, Seppala S, Yoo JI, Huyett LM, Pressman A, Cogan JZ, Kivenson V, Peng X, Tan Y, Valentine DL, O'Malley MA.	BMC Genomics	10.1186/s12864-017-4036-4	2017
	A phylogenetic and proteomic reconstruction of eukaryotic chromatin evolution.	Grau-Bove X, Navarrete C, Chiva C, Pribasnig T, Anto M, Torruella G, Galindo LJ, Lang BF, Moreira D, Lopez-Garcia P, Ruiz-Trillo I, Schleper C, Sabido E, Sebe-Pedros A.	Nat Ecol Evol	10.1038/s41559-022-01771-6	2022
Phylogeny	Methanosarcina spelaei sp. nov., a methanogenic archaeon isolated from a floating biofilm of a subsurface sulphurous lake.	Ganzert L, Schirmack J, Alawi M, Mangelsdorf K, Sand W, Hillebrand-Voiculescu A, Wagner D	Int J Syst Evol Microbiol	10.1099/ijs.0.064956-0	2014