Strain identifier

BacDive ID: 2375

Type strain: Yes

Species: Cellulomonas carbonis

Strain Designation: T26

Strain history: CGMCC 1.10786 <-- Z. Shi et al. T26.

NCBI tax ID(s): 1386092 (species)

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General

@ref: 18245

BacDive-ID: 2375

DSM-Number: 26485

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, motile, rod-shaped

description: Cellulomonas carbonis T26 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from subsurface soil of coal mine.

NCBI tax id

  • NCBI tax id: 1386092
  • Matching level: species

strain history

@refhistory
18245<- KCTC; KCC 19824
67770CGMCC 1.10786 <-- Z. Shi et al. T26.

doi: 10.13145/bacdive2375.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Cellulomonadaceae
  • genus: Cellulomonas
  • species: Cellulomonas carbonis
  • full scientific name: Cellulomonas carbonis Shi et al. 2012

  • synonyms

    • @ref: 20215
    • synonym: Actinotalea carbonis

@ref: 18245

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Cellulomonadaceae

genus: Cellulomonas

species: Cellulomonas carbonis

full scientific name: Cellulomonas carbonis Shi et al. 2012 emend. Nouioui et al. 2018

strain designation: T26

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
30392positive2.2 µm0.65 µmrod-shapedyes
69480positive100

pigmentation

  • @ref: 30392
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 18245
  • name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/514
  • composition: Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
18245positivegrowth28mesophilic
30392positivegrowth04-45
30392positiveoptimum28mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
30392positivegrowth06-10alkaliphile
30392positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 30392
  • oxygen tolerance: aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.987

halophily

  • @ref: 30392
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-7 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3039222599arabinose+carbon source
3039228757fructose+carbon source
3039228260galactose+carbon source
3039224265gluconate+carbon source
3039217234glucose+carbon source
3039228087glycogen+carbon source
3039217716lactose+carbon source
3039217306maltose+carbon source
3039237684mannose+carbon source
3039228053melibiose+carbon source
30392506227N-acetylglucosamine+carbon source
3039216634raffinose+carbon source
3039217814salicin+carbon source
3039230911sorbitol+carbon source
3039217992sucrose+carbon source
3039218222xylose+carbon source
303924853esculin+hydrolysis
3039217632nitrate+reduction

metabolite production

  • @ref: 30392
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: yes

enzymes

@refvalueactivityec
30392acid phosphatase+3.1.3.2
30392alpha-galactosidase+3.2.1.22
30392catalase+1.11.1.6
30392gelatinase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
18245subsurface soil of coal mineXijin District, TianjinChinaCHNAsia
67770Coal mine soilTianjinChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Other#Mine
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_87914.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_2302;97_2796;98_60750;99_87914&stattab=map
  • Last taxonomy: Cellulomonas
  • 16S sequence: HQ702749
  • Sequence Identity:
  • Total samples: 256
  • soil counts: 46
  • aquatic counts: 93
  • animal counts: 104
  • plant counts: 13

Safety information

risk assessment

  • @ref: 18245
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18245
  • description: Cellulomonas carbonis T26 16S ribosomal RNA gene, partial sequence
  • accession: HQ702749
  • length: 1442
  • database: ena
  • NCBI tax ID: 947969

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cellulomonas carbonis CGMCC 1.10786GCA_014636275scaffoldncbi1386092
66792Cellulomonas carbonis T26947969.5wgspatric947969
66792Cellulomonas carbonis strain CGMCC 1.107861386092.3wgspatric1386092
66792Cellulomonas carbonis T262636415627draftimg947969
67770Cellulomonas carbonis T26GCA_000767175contigncbi947969

GC content

@refGC-contentmethod
1824574.4
6777074.4high performance liquid chromatography (HPLC)
6777073genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes74no
flagellatedno89.266no
gram-positiveyes93.501no
anaerobicno99.219yes
aerobicyes88.961yes
halophileno87.888no
spore-formingno84.687no
glucose-utilyes84.806yes
thermophileno96.994yes
motileyes86.452yes
glucose-fermentno75.454no

External links

@ref: 18245

culture collection no.: DSM 26485, CCTCC AB 2010450, CGMCC 1.10786, KCTC 19824, JCM 19664, NBRC 110798

straininfo link

  • @ref: 71930
  • straininfo: 400957

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22021576Cellulomonas carbonis sp. nov., isolated from coal mine soil.Shi Z, Luo G, Wang GInt J Syst Evol Microbiol10.1099/ijs.0.034934-02011Bacterial Typing Techniques, Base Composition, Cellulomonas/*classification/genetics/isolation & purification, China, Coal Mining, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil/analysis, *Soil MicrobiologyGenetics
Phylogeny29458505Actinotalea solisilvae sp. nov., isolated from forest soil and emended description of the genus Actinotalea.Yan ZF, Lin P, Li CT, Kook M, Yi THInt J Syst Evol Microbiol10.1099/ijsem.0.0025842018Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, Glycolipids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
18245Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26485)https://www.dsmz.de/collection/catalogue/details/culture/DSM-26485
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30392Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2672928776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71930Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID400957.1StrainInfo: A central database for resolving microbial strain identifiers