Strain identifier

BacDive ID: 2367

Type strain: Yes

Species: Cellulomonas denverensis

Strain Designation: W6929

Strain history: CIP <- 2005, J.M. Brown, CDC, Atlanta, USA: strain W6929

NCBI tax ID(s): 1305869 (strain), 264297 (species)

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General

@ref: 6104

BacDive-ID: 2367

DSM-Number: 15764

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, human pathogen

description: Cellulomonas denverensis W6929 is a facultative anaerobe, spore-forming, mesophilic human pathogen that was isolated from blood.

NCBI tax id

NCBI tax idMatching level
264297species
1305869strain

strain history

@refhistory
6104<- J. M. Brown; W6929 <- University of Colorado Medical Center
349822005, J.M. Brown, CDC, Atlanta, USA: strain W6929
67770CIP 108914 <-- J. M. Brown W6929 <-- Univ. Colorado, Med. Center, USA.
118915CIP <- 2005, J.M. Brown, CDC, Atlanta, USA: strain W6929

doi: 10.13145/bacdive2367.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Cellulomonadaceae
  • genus: Cellulomonas
  • species: Cellulomonas denverensis
  • full scientific name: Cellulomonas denverensis Brown et al. 2005

@ref: 6104

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Cellulomonadaceae

genus: Cellulomonas

species: Cellulomonas denverensis

full scientific name: Cellulomonas denverensis Brown et al. 2005

strain designation: W6929

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
118915positiverod-shapedyes

colony morphology

  • @ref: 118915
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6104TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34982MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
118915CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6104positivegrowth28mesophilic
34982positivegrowth37mesophilic
67770positivegrowth37mesophilic
118915positivegrowth15-45
118915nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 118915
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
69481yes100
69480no99.949

halophily

  • @ref: 118915
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11891516947citrate-carbon source
1189154853esculin+hydrolysis
118915606565hippurate+hydrolysis
11891517632nitrate+reduction
11891516301nitrite-reduction
11891517632nitrate+respiration

metabolite production

  • @ref: 118915
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11891515688acetoin-
11891517234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
118915oxidase+
118915beta-galactosidase+3.2.1.23
118915alcohol dehydrogenase-1.1.1.1
118915gelatinase+/-
118915amylase+
118915DNase+
118915caseinase+3.4.21.50
118915catalase+1.11.1.6
118915tween esterase-
118915gamma-glutamyltransferase+2.3.2.2
118915lecithinase-
118915lipase-
118915lysine decarboxylase-4.1.1.18
118915ornithine decarboxylase-4.1.1.17
118915phenylalanine ammonia-lyase-4.3.1.24
118915protease+
118915tryptophan deaminase-
118915urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118915--+--+-----+-+-+++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
6104bloodDenverUSAUSANorth America
46835Human bloodColorado,DenverUSAUSANorth America1972-05-01
67770Human bloodDenver, COUSAUSANorth America
118915Human, BloodDenver, ColoradoUnited States of AmericaUSANorth America1975

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Fluids#Blood
#Infection#Patient

taxonmaps

  • @ref: 69479
  • File name: preview.99_16218.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_432;97_493;98_12225;99_16218&stattab=map
  • Last taxonomy: Cellulomonas denverensis
  • 16S sequence: AY501362
  • Sequence Identity:
  • Total samples: 807
  • soil counts: 235
  • aquatic counts: 66
  • animal counts: 318
  • plant counts: 188

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
6104yes, in single cases1Risk group (German classification)
1189151Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6104
  • description: Cellulomonas denverensis 16S small subunit ribosomal RNA gene, partial sequence
  • accession: AY501362
  • length: 1422
  • database: ena
  • NCBI tax ID: 264297

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cellulomonas denverensis NBRC 110783GCA_016862695contigncbi264297
66792Cellulomonas denverensis JCM 14733GCA_001315285contigncbi1305869
66792Cellulomonas denverensis JCM 147331305869.3wgspatric1305869
66792Cellulomonas denverensis strain NBRC 110783264297.6wgspatric264297
66792Cellulomonas denverensis JCM 147332734481980draftimg1305869
67770Cellulomonas denverensis ATCC BAA-788GCA_012396125contigncbi264297

GC content

@refGC-contentmethod
610468.5
6777068.5thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno50no
gram-positiveyes89.01no
anaerobicno95.926no
halophileno89.145no
spore-formingno92.536no
thermophileno98.882yes
glucose-utilyes86.466no
aerobicno50.617no
flagellatedno92.752no
glucose-fermentyes64.613no

External links

@ref: 6104

culture collection no.: DSM 15764, ATCC BAA 788, NBRC 110783, CCUG 18431, JCM 14733, CIP 108914

straininfo link

  • @ref: 71922
  • straininfo: 52577

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15814993Phenotypic and genetic characterization of clinical isolates of CDC coryneform group A-3: proposal of a new species of Cellulomonas, Cellulomonas denverensis sp. nov.Brown JM, Frazier RP, Morey RE, Steigerwalt AG, Pellegrini GJ, Daneshvar MI, Hollis DG, McNeil MMJ Clin Microbiol10.1128/JCM.43.4.1732-1737.20052005Actinomycetales Infections/microbiology, *Bacterial Typing Techniques, Cellulomonas/chemistry/*classification/*genetics/isolation & purification, DNA, Ribosomal/analysis, Fatty Acids/analysis, Humans, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAPathogenicity
Phylogeny35779121Cellulomonas triticagri sp. nov., isolated from the rhizosphere soil of wheat (Triticum aestivum L.).Han C, Zhang Y, Yu B, Shan Q, Zhao J, Shi H, Tian Y, Zhang Y, Zhu C, Xiang WArch Microbiol10.1007/s00203-022-03036-z2022Bacterial Typing Techniques, *Cellulomonas/genetics, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, Soil/chemistry, Soil Microbiology, TriticumTranscriptome

Reference

@idauthorscataloguedoi/urltitle
6104Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15764)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15764
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
34982Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6597
46835Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 18431)https://www.ccug.se/strain?id=18431
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71922Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID52577.1StrainInfo: A central database for resolving microbial strain identifiers
118915Curators of the CIPCollection of Institut Pasteur (CIP 108914)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108914