Strain identifier
BacDive ID: 23549
Type strain: 
Species: Eoetvoesiella caeni
Strain Designation: PB3-7B
Strain history: <- T. Felföldi, Eötvös Loránd Univ. Budapest, Hungary; PB3-7B
NCBI tax ID(s): 645616 (species)
version 9.1 (current version)
General
@ref: 21341
BacDive-ID: 23549
DSM-Number: 25520
keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, Gram-positive, motile, rod-shaped
description: Eoetvoesiella caeni PB3-7B is a facultative anaerobe, spore-forming, Gram-positive bacterium that was isolated from activated sludge of a reactor treating coke plant effluent.
NCBI tax id
- NCBI tax id: 645616
- Matching level: species
strain history
- @ref: 21341
- history: <- T. Felföldi, Eötvös Loránd Univ. Budapest, Hungary; PB3-7B
doi: 10.13145/bacdive23549.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Alcaligenaceae
- genus: Eoetvoesiella
- species: Eoetvoesiella caeni
- full scientific name: Eoetvoesiella caeni (Felföldi et al. 2014) Deshmukh and Oren 2023
synonyms
- @ref: 20215
- synonym: Eoetvoesia caeni
@ref: 21341
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Alcaligenaceae
genus: Eoetvoesia
species: Eoetvoesia caeni
full scientific name: Eoetvoesia caeni Felföldi et al. 2014
strain designation: PB3-7B
type strain: yes
Morphology
cell morphology
- @ref: 31178
- gram stain: positive
- cell shape: rod-shaped
- motility: yes
Culture and growth conditions
culture medium
- @ref: 21341
- name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535c)
- growth: yes
- link: https://mediadive.dsmz.de/medium/535c
- composition: Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535c) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Tween 80 10.0 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 21341 | positive | growth | 28 |
| 31178 | positive | growth | 35-70 |
| 31178 | positive | optimum | 55 |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 31178 | positive | growth | 6.5-9.0 | alkaliphile |
| 31178 | positive | optimum | 8.0-8.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 31178
- oxygen tolerance: facultative anaerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 31178 | yes | |
| 69481 | yes | 100 |
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 31178 | NaCl | positive | growth | 0-2.5 % |
| 31178 | NaCl | positive | optimum | 0.5 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 31178 | 17716 | lactose | + | carbon source |
| 31178 | 29864 | mannitol | + | carbon source |
| 31178 | 33942 | ribose | + | carbon source |
| 31178 | 17992 | sucrose | + | carbon source |
Isolation, sampling and environmental information
isolation
- @ref: 21341
- sample type: activated sludge of a reactor treating coke plant effluent
- geographic location: Budapest, Eötvös Loránd University
- country: Hungary
- origin.country: HUN
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Bioreactor | |
| #Engineered | #Waste | #Activated sludge |
| #Engineered | #Waste | #Industrial wastewater |
taxonmaps
- @ref: 69479
- File name: preview.99_28364.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_7;96_2229;97_17071;98_21205;99_28364&stattab=map
- Last taxonomy: Eoetvoesia caeni subclade
- 16S sequence: FJ948170
- Sequence Identity:
- Total samples: 151
- soil counts: 51
- aquatic counts: 58
- animal counts: 28
- plant counts: 14
Safety information
risk assessment
- @ref: 21341
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21341
- description: Eoetvoesia caeni strain PB3-7B 16S ribosomal RNA gene, partial sequence
- accession: FJ948170
- length: 1453
- database: nuccore
- NCBI tax ID: 645616
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Eoetvoesiella caeni PB3-7B | GCA_013416545 | contig | ncbi | 645616 |
| 66792 | Eoetvoesiella caeni DSM 25520 | GCA_003315175 | scaffold | ncbi | 645616 |
| 66792 | Eoetvoesia caeni strain DSM 25520 | 645616.3 | wgs | patric | 645616 |
| 66792 | Eoetvoesia caeni strain PB3-7B | 645616.4 | wgs | patric | 645616 |
| 66792 | Eoetvoesia caeni DSM 25520 | 2770939557 | draft | img | 645616 |
| 66792 | Eoetvoesiella caeni PB3-7B | GCA_022688825 | contig | ncbi | 645616 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 31178 | 37.8 | |
| 21341 | 59.7 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | yes | 100 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 70.378 | yes |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 97.167 | yes |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 75.478 | yes |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 67.791 | yes |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | no | 96.417 | no |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | yes | 82.83 | no |
External links
@ref: 21341
culture collection no.: DSM 25520, NCAIM B 02512
straininfo link
- @ref: 87644
- straininfo: 407081
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 24585374 | Eoetvoesia caeni gen. nov., sp. nov., isolated from an activated sludge system treating coke plant effluent. | Felfoldi T, Vengring A, Keki Z, Marialigeti K, Schumann P, Toth EM | Int J Syst Evol Microbiol | 10.1099/ijs.0.058875-0 | 2014 | Alcaligenaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, *Coke, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hungary, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/chemistry, Waste Water/*microbiology | Genetics |
| Phylogeny | 32369004 | Pusillimonas maritima sp. nov., isolated from surface seawater. | Li J, Qi M, Lai Q, Dong C, Liu X, Wang G, Shao Z | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004202 | 2020 | Alcaligenaceae/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Indian Ocean, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*chemistry, Sequence Analysis, DNA, Ubiquinone/chemistry | Transcriptome |
Reference
| @id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||||
| 21341 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25520 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25520) | ||||
| 31178 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 27505 | 28776041 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | ||||
| 87644 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID407081.1 |