Strain identifier

BacDive ID: 23319

Type strain: Yes

Species: Acrocarpospora phusangensis

Strain Designation: PS33-18

Strain history: <- C Suriyachadkun, BCC; BCC 46906 <- N. Niemhom; PS33-18

NCBI tax ID(s): 1070424 (species)

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General

@ref: 19331

BacDive-ID: 23319

DSM-Number: 45867

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, Gram-positive

description: Acrocarpospora phusangensis PS33-18 is a spore-forming, Gram-positive bacterium that builds an aerial mycelium.

NCBI tax id

  • NCBI tax id: 1070424
  • Matching level: species

strain history

  • @ref: 19331
  • history: <- C Suriyachadkun, BCC; BCC 46906 <- N. Niemhom; PS33-18

doi: 10.13145/bacdive23319.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Streptosporangiaceae
  • genus: Acrocarpospora
  • species: Acrocarpospora phusangensis
  • full scientific name: Acrocarpospora phusangensis Niemhom et al. 2013

@ref: 19331

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptosporangiaceae

genus: Acrocarpospora

species: Acrocarpospora phusangensis

full scientific name: Acrocarpospora phusangensis Niemhom et al. 2013

strain designation: PS33-18

type strain: yes

Morphology

cell morphology

  • @ref: 30863
  • gram stain: positive

colony morphology

@refcolony colormedium used
69449Ivory (1014)ISP 4
69449Light ivory (1015)ISP 3
69449Light ivory (1015)ISP 5
69449Light ivory (1015)ISP 7
69449Light ivory (1015), brown beige (1011)suter with tyrosine
69449Light ivory (1015), olive yellow (1020)suter without tyrosine
69449Sand yellow (1002)ISP 2

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69449noAerial myceliumISP 2
69449yesAerial myceliumISP 3Cream (9001)
69449noAerial myceliumISP 4
69449noAerial myceliumISP 5
69449yesAerial myceliumISP 7Cream (9001)
69449noAerial myceliumsuter with tyrosine
69449noAerial myceliumsuter without tyrosine

pigmentation

@refproductionname
30863no
69449noMelanin
69449nosoluble pigment

multimedia

@refmultimedia contentcaptionintellectual property rights
69449DSM_45867_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69449DSM_45867_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
19331ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf
19331GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperature
19331positivegrowth28
30863positivegrowth25-30
30863positiveoptimum27.5

culture pH

  • @ref: 30863
  • ability: positive
  • type: growth
  • pH: 07-11
  • PH range: alkaliphile

Physiology and metabolism

spore formation

  • @ref: 69481
  • spore formation: yes
  • confidence: 100

halophily

@refsaltgrowthtested relationconcentration
30863NaClpositiveoptimum0-1 %
69449NaClpositivegrowth0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3086322599arabinose+carbon source
3086317057cellobiose+carbon source
3086328757fructose+carbon source
3086328260galactose+carbon source
3086317234glucose+carbon source
3086317716lactose+carbon source
3086317306maltose+carbon source
3086329864mannitol+carbon source
3086328053melibiose+carbon source
3086316634raffinose+carbon source
3086326546rhamnose+carbon source
3086317992sucrose+carbon source
6944922599arabinose+/-growth
6944962968cellulose+/-growth
6944928757fructose+growth
6944917234glucose+growth
6944917268inositol+growth
6944937684mannose+growth
6944916634raffinose+/-growth
6944926546rhamnose+growth
6944917992sucrose+growth
6944918222xylose+growth
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69449---+-+++++---------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69449++--++---++-+-+/--+--

Isolation, sampling and environmental information

isolation

  • @ref: 19331
  • geographic location: Phayao
  • country: Thailand
  • origin.country: THA
  • continent: Asia

Safety information

risk assessment

  • @ref: 19331
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19331
  • description: Acrocarpospora phusangensis gene for 16S ribosomal RNA, partial sequence
  • accession: AB649124
  • length: 1453
  • database: nuccore
  • NCBI tax ID: 1070424

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acrocarpospora phusangensis NBRC 108782GCA_016862995contigncbi1070424
66792Acrocarpospora phusangensis strain NBRC 1087821070424.3wgspatric1070424

GC content

  • @ref: 19331
  • GC-content: 71

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes89.831yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no93.316no
69480spore-formingspore-formingAbility to form endo- or exosporesyes89.99no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes88.551no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.5yes
69480flagellatedmotile2+Ability to perform flagellated movementno82no

External links

@ref: 19331

culture collection no.: DSM 45867, BCC 46906, NBRC 108782

straininfo link

  • @ref: 87537
  • straininfo: 403245

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23125318Acrocarpospora phusangensis sp. nov., isolated from a temperate peat swamp forest soil.Niemhom N, Suriyachadkun C, Tamura T, Thawai CInt J Syst Evol Microbiol10.1099/ijs.0.046227-02012Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/analogs & derivatives/analysis, *WetlandsGenetics
Phylogeny36129751Acrocarpospora catenulata sp. nov., a novel actinobacterium isolated from lake sediment.Liu C, Guo Y, Li L, Li J, Wang L, Lin J, Wang X, Chu YInt J Syst Evol Microbiol10.1099/ijsem.0.0055262022*Actinomycetales, Bacterial Typing Techniques, Base Composition, Cardiolipins, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glucose, Lakes/analysis, Nucleotides, *Phosphatidylethanolamines, Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Ribose, Sequence Analysis, DNA, Soil Microbiology, Vitamin K 2/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
19331Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45867)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45867
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30863Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2719328776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69449Wink, J.https://cdn.dsmz.de/wink/DSM%2045867.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
87537Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID403245.1StrainInfo: A central database for resolving microbial strain identifiers