Strain identifier
BacDive ID: 23266
Type strain: 
Species: Novosphingobium fuchskuhlense
Strain Designation: FNE08-7
Strain history: <- S. P. Glaeser, Justus-Liebig-Universität, Giessen, Germany; FNE08-7
NCBI tax ID(s): 1117702 (species)
version 8.1 (current version)
General
@ref: 19230
BacDive-ID: 23266
DSM-Number: 25065
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped
description: Novosphingobium fuchskuhlense FNE08-7 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from subsurface water of a humic acid rich bog lake.
NCBI tax id
- NCBI tax id: 1117702
- Matching level: species
strain history
- @ref: 19230
- history: <- S. P. Glaeser, Justus-Liebig-Universität, Giessen, Germany; FNE08-7
doi: 10.13145/bacdive23266.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Sphingomonadales
- family: Erythrobacteraceae
- genus: Novosphingobium
- species: Novosphingobium fuchskuhlense
- full scientific name: Novosphingobium fuchskuhlense Glaeser et al. 2013
@ref: 19230
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Sphingomonadales
family: Erythrobacteraceae
genus: Novosphingobium
species: Novosphingobium fuchskuhlense
full scientific name: Novosphingobium fuchskuhlense Glaeser et al. 2013
strain designation: FNE08-7
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 30724 | negative | 1.7 µm | 0.8 µm | rod-shaped | no | |
| 69480 | negative | 99.966 |
colony morphology
- @ref: 62645
- incubation period: 2-3 days
pigmentation
- @ref: 30724
- production: yes
Culture and growth conditions
culture medium
- @ref: 19230
- name: REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c)
- growth: yes
- link: https://mediadive.dsmz.de/medium/830c
- composition: Name: REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 19230 | positive | growth | 28 | mesophilic |
| 30724 | positive | growth | 04-32 | |
| 30724 | positive | optimum | 25 | mesophilic |
| 62645 | positive | growth | 20 | psychrophilic |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 30724 | positive | growth | 05-07 |
| 30724 | positive | optimum | 6.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 62645
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 96 |
| 69480 | no | 99.998 |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30724 | 17057 | cellobiose | + | carbon source |
| 30724 | 17234 | glucose | + | carbon source |
| 30724 | 27570 | histidine | + | carbon source |
| 30724 | 24996 | lactate | + | carbon source |
| 30724 | 15361 | pyruvate | + | carbon source |
| 30724 | 26546 | rhamnose | + | carbon source |
enzymes
- @ref: 30724
- value: cytochrome oxidase
- activity: +
- ec: 1.9.3.1
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | sampling date |
|---|---|---|---|---|---|---|---|---|
| 19230 | subsurface water of a humic acid rich bog lake | Stechlin, Lake Große Fuchskuhle, north eastern basin | Germany | DEU | Europe | 53.1667 | 13.0333 | |
| 62645 | Surface water | Lake Grosse Fuchskuhle | Germany | DEU | Europe | 2008-06-20 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Environmental | #Terrestrial | #Wetland (Swamp) |
| #Condition | #Acidic |
taxonmaps
- @ref: 69479
- File name: preview.99_713.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_20;96_437;97_499;98_573;99_713&stattab=map
- Last taxonomy: Novosphingobium
- 16S sequence: JN399172
- Sequence Identity:
- Total samples: 4437
- soil counts: 173
- aquatic counts: 3763
- animal counts: 384
- plant counts: 117
Safety information
risk assessment
- @ref: 19230
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 19230
- description: Novosphingobium fuchskuhlense strain FNE08-7 16S ribosomal RNA gene, partial sequence
- accession: JN399172
- length: 1338
- database: ena
- NCBI tax ID: 1117702
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Novosphingobium fuchskuhlense FNE08-7 | GCA_001519075 | scaffold | ncbi | 1117702 |
| 66792 | Novosphingobium fuchskuhlense strain FNE08-7 | 1117702.3 | wgs | patric | 1117702 |
| 66792 | Novosphingobium fuchskuhlense FNE08-7 | 2775506978 | draft | img | 1117702 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 96 | no |
| flagellated | no | 89.171 | yes |
| gram-positive | no | 97.364 | yes |
| anaerobic | no | 99.119 | yes |
| aerobic | yes | 93.804 | yes |
| halophile | no | 93.436 | no |
| spore-forming | no | 95.523 | no |
| glucose-util | yes | 84.969 | yes |
| motile | yes | 50.735 | yes |
| glucose-ferment | no | 93.893 | no |
| thermophile | no | 91.656 | yes |
External links
@ref: 19230
culture collection no.: DSM 25065, CCM 7978, CCUG 61508
straininfo link
- @ref: 87489
- straininfo: 398390
literature
- topic: Phylogeny
- Pubmed-ID: 22544783
- title: Novosphingobium fuchskuhlense sp. nov., isolated from the north-east basin of Lake Grosse Fuchskuhle.
- authors: Glaeser SP, Bolte K, Martin K, Busse HJ, Grossart HP, Kampfer P, Glaeser J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.043083-0
- year: 2012
- mesh: Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Germany, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sphingomonadaceae/*classification/genetics/isolation & purification, Ubiquinone/analysis, *Water Microbiology
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 19230 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25065) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25065 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 30724 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27055 | 28776041 | |
| 62645 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 61508) | https://www.ccug.se/strain?id=61508 | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 87489 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID398390.1 | StrainInfo: A central database for resolving microbial strain identifiers |