Strain identifier

BacDive ID: 23082

Type strain: Yes

Species: Methanothermobacter tenebrarum

Strain Designation: RMAS

Culture col. no.: DSM 23052, JCM 16532, NBRC 106236

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 19215

BacDive-ID: 23082

DSM-Number: 23052

keywords: thermophilic, anaerobe, Archaea, 16S sequence, genome sequence, gram-positive, rod-shaped

description: Methanothermobacter tenebrarum RMAS is an anaerobe, thermophilic, gram-positive archaeon that was isolated from gas-associated formation water from a well of natural gas field.

strain history: <- K. Nakamura, Faculty Applied Biological Sci., Gifu Univ., Japan; RMAS <- A. Takahashi {2008}

doi: 10.13145/bacdive23082.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/euryarchaeota
  • domain: Archaea
  • phylum: Euryarchaeota
  • class: Methanobacteria
  • order: Methanobacteriales
  • family: Methanobacteriaceae
  • genus: Methanothermobacter
  • species: Methanothermobacter tenebrarum
  • full scientific name: Methanothermobacter tenebrarum Nakamura et al. 2013

@ref: 19215

domain: Archaea

phylum: Euryarchaeota

class: Methanobacteria

order: Methanobacteriales

family: Methanobacteriaceae

genus: Methanothermobacter

species: Methanothermobacter tenebrarum

full scientific name: Methanothermobacter tenebrarum Nakamura et al. 2013

strain designation: RMAS

type strain: yes

Morphology

cell morphology

  • @ref: 30672
  • gram stain: positive
  • cell length: 7 µm
  • cell width: 0.5 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 19215
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

  • @ref: 19215
  • name: METHANOBACTERIUM MEDIUM (DSMZ Medium 119), anaerobic
  • growth: yes
  • link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf

culture temp

@refgrowthtypetemperaturerange
19215positivegrowth70thermophilic
30672positivegrowth45-80hyperthermophilic
30672positiveoptimum70thermophilic

culture pH

@refabilitytypepH
30672positivegrowth5.8-8.7
30672positiveoptimum7.3

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
19215anaerobe
30672anaerobe

halophily

  • @ref: 30672
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-2 %

Isolation, sampling and environmental information

isolation

  • @ref: 19215
  • sample type: gas-associated formation water from a well of natural gas field
  • geographic location: Niigata
  • country: Japan
  • origin.country: JPN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic
#Environmental#Terrestrial#Geologic

Safety information

risk assessment

  • @ref: 19215
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19215
  • description: Methanothermobacter tenebrarum gene for 16S rRNA, partial sequence
  • accession: AB523785
  • length: 1414
  • database: ena
  • NCBI tax ID: 680118

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methanothermobacter tenebrarum MCM B 1447GCA_003264935contigncbi680118
66792Methanothermobacter tenebrarum strain MCM B 1447680118.3wgspatric680118

GC content

@refGC-contentmethod
1921541.5high performance liquid chromatography (HPLC)
3067241.5

External links

@ref: 19215

culture collection no.: DSM 23052, JCM 16532, NBRC 106236

straininfo link

@refpassport
20218http://www.straininfo.net/strains/884890
20218http://www.straininfo.net/strains/884891
20218http://www.straininfo.net/strains/884892

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
19215Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23052)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23052
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2700310.1099/ijs.0.041681-0
30672Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information27003
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)