Name: Methanothermobacter tenebrarum RMAS
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23082

Type strain:

Species: Methanothermobacter tenebrarum

Strain Designation: RMAS

Culture col. no.: DSM 23052, JCM 16532, NBRC 106236

Strain history: K. Nakamura strain RMAS.

NCBI tax ID(s): 680118 (species)

SI-ID 398257

DSM 23052

Section

Methanothermobacter tenebrarum RMAS is an anaerobe, thermophilic, Gram-positive archaeon that was isolated from gas-associated formation water from a well of natural gas field.

Gram-positive
rod-shaped
anaerobe
thermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Methanobacteria*
	Order	*Methanobacteriales*
	Family	*Methanobacteriaceae*
	Genus	*Methanothermobacter*
	Species	**Methanothermobacter tenebrarum**
	Full Scientific Name (LPSN)	Methanothermobacter tenebrarum Nakamura et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30672]	Gram stain	positive

[Ref.: #30672]	Cell length	7 µm

[Ref.: #30672]	Cell width	0.5 µm

[Ref.: #30672]	Cell shape	rod-shaped

[Ref.: #30672]	Motility	no

[Ref.: #19215]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19215]

Culture medium

METHANOBACTERIUM MEDIUM (DSMZ Medium 119)

[Ref.: #19215]

Culture medium growth

[Ref.: #19215]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19215]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #19215]	growth	70 °C
[Ref.: #30672]	growth	45-80 °C
[Ref.: #30672]	optimum	70 °C
[Ref.: #67770]	growth	65 °C

[Ref.: #19215]	Temperature range	thermophilic
[Ref.: #30672]	Temperature range	hyperthermophilic
[Ref.: #30672]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #30672]			optimum	7.3
[Ref.: #30672]			growth	5.8-8.7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #19215]	Oxygen tolerance	anaerobe
[Ref.: #30672]	Oxygen tolerance	anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30672]		NaCl		growth	0-2	%

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19215]	Sample type/isolated from	gas-associated formation water from a well of natural gas field
[Ref.: #19215]	Geographic location (country and/or sea, region)	Niigata
[Ref.: #19215]	Country	Japan
[Ref.: #19215]	Country ISO 3 Code	JPN
[Ref.: #19215]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Gas-associated formation water of a gas-producing well in a natural gas field
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	-
#Environmental	#Terrestrial	#Geologic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB523785 (>99% sequence identity) for Methanothermobacter tenebrarum subclade from Microbeatlas

Aquatic Samples	42
Soil Samples	3
Animal Samples
Plant Samples	1
Total Samples	46

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19215]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #19215]	Methanothermobacter tenebrarum gene for 16S rRNA, partial sequence	AB523785	1414	ENA	680118 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanothermobacter tenebrarum RMAS	GCA_023167465	complete	GenBank	680118 tax ID	*

[Ref.: #19215]	GC-content	41.5 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30672]	GC-content	41.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanothermobacter tenebrarum RMAS NCBI: GCA_023167465.1
[Ref.: #69480]	flagellated	no	96.543	yes
[Ref.: #69480]	gram-positive	no	96.43	yes
[Ref.: #69480]	anaerobic	yes	98.929	yes
[Ref.: #69480]	aerobic	no	97.384	yes
[Ref.: #69480]	halophile	no	80.844	no
[Ref.: #69480]	spore-forming	no	96.764	no
[Ref.: #69480]	motile	no	87.108	no
[Ref.: #69480]	glucose-ferment	no	63.002	no
[Ref.: #69480]	thermophile	yes	96.407	yes
[Ref.: #69480]	glucose-util	yes	73.526	no

Availability in culture collections External links

[Ref.: #19215]

Culture collection no.

DSM 23052, JCM 16532, NBRC 106236

[Ref.: #87320]

SI-ID 398257

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanothermobacter tenebrarum sp. nov., a hydrogenotrophic, thermophilic methanogen isolated from gas-associated formation water of a natural gas field.	Nakamura K, Takahashi A, Mori C, Tamaki H, Mochimaru H, Nakamura K, Takamizawa K, Kamagata Y	Int J Syst Evol Microbiol	10.1099/ijs.0.041681-0	2012	*

References

#19215

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23052

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30672

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27003 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#87320

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398257.1 )

#27003

IJSEM 715 2013 ( DOI 10.1099/ijs.0.041681-0 , PubMed 22561588 )

* These data were automatically processed and therefore are not curated

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