Strain identifier

BacDive ID: 22940

Type strain: Yes

Species: Brucella daejeonensis

Strain Designation: MJ11

Strain history: M. J. Lee MJ11.

NCBI tax ID(s): 659015 (species)

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General

@ref: 19127

BacDive-ID: 22940

DSM-Number: 26944

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative

description: Brucella daejeonensis MJ11 is a mesophilic, Gram-negative bacterium that was isolated from sludge of a leachate treatment plant.

NCBI tax id

  • NCBI tax id: 659015
  • Matching level: species

strain history

@refhistory
19127<- JCM <- M.-J. Lee; MJ11
67770M. J. Lee MJ11.

doi: 10.13145/bacdive22940.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Brucellaceae
  • genus: Brucella
  • species: Brucella daejeonensis
  • full scientific name: Brucella daejeonensis (Woo et al. 2011) Hördt et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Ochrobactrum daejeonense

@ref: 19127

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Brucellaceae

genus: Brucella

species: Brucella daejeonensis

full scientific name: Brucella daejeonensis (Woo et al. 2011) Hördt et al. 2020

strain designation: MJ11

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.998

colony morphology

  • @ref: 19127
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
19127TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
19127CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
19127positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.996

observation

  • @ref: 67770
  • observation: quinones: Q-10

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836924265gluconate-assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
19127catalase+1.11.1.6
19127cytochrome-c oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
19127+---+/----++++++-+/--+-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
19127sludge of a leachate treatment plantDaejeonRepublic of KoreaKORAsia
67770Sludge of the aerobic treatment tanks of a municipal leachate treatment plantDaejeonRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Water treatment plant
#Environmental#Terrestrial#Mud (Sludge)

Safety information

risk assessment

  • @ref: 19127
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 19127
  • description: Ochrobactrum daejeonense strain MJ11 16S ribosomal RNA gene, partial sequence
  • accession: HQ171203
  • length: 1389
  • database: ena
  • NCBI tax ID: 659015

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brucella daejeonensis DSM 26944GCA_014199265contigncbi659015
66792Ochrobactrum daejeonense strain DSM 26944659015.4wgspatric659015
66792Ochrobactrum daejeonense strain JCM 16234659015.3wgspatric659015
66792Brucella daejeonensis DSM 269442828382032draftimg659015
67770Brucella daejeonensis JCM 16234GCA_012103095contigncbi659015

GC content

  • @ref: 19127
  • GC-content: 59.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
gram-positiveno98.378no
anaerobicno97.287no
halophileno92.208no
spore-formingno96.892no
glucose-utilyes90.59yes
thermophileno97.126no
flagellatedno83.665no
motileyes79.288no
aerobicyes85.9no
glucose-fermentno89.455yes

External links

@ref: 19127

culture collection no.: DSM 26944, JCM 16234, KCTC 22458

straininfo link

  • @ref: 87188
  • straininfo: 405163

literature

  • topic: Phylogeny
  • Pubmed-ID: 21169456
  • title: Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant.
  • authors: Woo SG, Ten LN, Park J, Lee M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.025510-0
  • year: 2010
  • mesh: Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Nitrates/*metabolism, Ochrobactrum/*classification/genetics/*isolation & purification/metabolism, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sewage/*microbiology, Waste Disposal, Fluid
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitle
19127Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26944)https://www.dsmz.de/collection/catalogue/details/culture/DSM-26944
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
87188Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405163.1StrainInfo: A central database for resolving microbial strain identifiers