Name: Brucella daejeonensis MJ11
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 22940

Type strain:

Species: Brucella daejeonensis

Strain Designation: MJ11

Culture col. no.: DSM 26944, JCM 16234, KCTC 22458

Strain history: M. J. Lee MJ11.

NCBI tax ID(s): 659015 (species)

SI-ID 405163

DSM 26944

Section

Brucella daejeonensis MJ11 is a mesophilic, Gram-negative bacterium that was isolated from sludge of a leachate treatment plant.

Gram-negative
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Brucellaceae*
	Genus	*Brucella*
	Species	**Brucella daejeonensis**
	Full Scientific Name (LPSN)	Brucella daejeonensis (Woo et al. 2011) Hördt et al. 2020

Synonym

Ochrobactrum daejeonense

Information on morphological and physiological properties Morphology

[Ref.: #69480]

Gram stain

negative Confidence in %99.998

[Ref.: #19127]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19127]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #19127]

Culture medium growth

[Ref.: #19127]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19127]

Culture medium composition

expand / minimize

[Ref.: #19127]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #19127]

Culture medium growth

[Ref.: #19127]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19127]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #19127]			growth	30 °C
[Ref.: #67770]			growth	30 °C

[Ref.: #19127]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996

[Ref.: #67770]

Observation

quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #19127]	catalase	+	1.11.1.6
[Ref.: #19127]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68369]	gelatinase	-		* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #19127]	23968	+	-	-	-	+/-	-	-	-	+	+	+	+	+	+	-	+/-	-	+	-	+	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19127]	Sample type/isolated from	sludge of a leachate treatment plant
[Ref.: #19127]	Geographic location (country and/or sea, region)	Daejeon
[Ref.: #19127]	Country	Republic of Korea
[Ref.: #19127]	Country ISO 3 Code	KOR
[Ref.: #19127]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Sludge of the aerobic treatment tanks of a municipal leachate treatment plant
[Ref.: #67770]	Geographic location (country and/or sea, region)	Daejeon
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	#Water treatment plant
#Environmental	#Terrestrial	#Mud (Sludge)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19127]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #19127]	Ochrobactrum daejeonense strain MJ11 16S ribosomal RNA gene, partial sequence	HQ171203	1389	ENA	659015 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Brucella daejeonensis JCM 16234	GCA_012103095	contig	GenBank	659015 tax ID
[Ref.: #66792]	Brucella daejeonensis DSM 26944	GCA_014199265	contig	GenBank	659015 tax ID	*
[Ref.: #66792]	Ochrobactrum daejeonense strain DSM 26944	659015.4	wgs	PATRIC	659015 tax ID	*
[Ref.: #66792]	Ochrobactrum daejeonense strain JCM 16234	659015.3	wgs	PATRIC	659015 tax ID	*
[Ref.: #66792]	Brucella daejeonensis DSM 26944	2828382032	draft	JGI IMG/M	659015 tax ID	*

[Ref.: #19127]

GC-content

59.3 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Ochrobactrum daejeonense strain JCM 16234 PATRIC: 659015.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ochrobactrum daejeonense strain DSM 26944 PATRIC: 659015.4
[Ref.: #69480]	gram-positive	no	98.378	no
[Ref.: #69480]	anaerobic	no	97.287	no
[Ref.: #69480]	halophile	no	92.208	no
[Ref.: #69480]	spore-forming	no	96.892	no
[Ref.: #69480]	glucose-util	yes	90.59	yes
[Ref.: #69480]	thermophile	no	97.126	no
[Ref.: #69480]	flagellated	no	83.665	no
[Ref.: #69480]	motile	yes	79.288	no
[Ref.: #69480]	aerobic	yes	85.9	no
[Ref.: #69480]	glucose-ferment	no	89.455	yes

Availability in culture collections External links

[Ref.: #19127]

Culture collection no.

DSM 26944, JCM 16234, KCTC 22458

[Ref.: #87188]

SI-ID 405163

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant.	Woo SG, Ten LN, Park J, Lee M	Int J Syst Evol Microbiol	10.1099/ijs.0.025510-0	2010	*

References

#19127

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26944

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87188

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405163.1 )

* These data were automatically processed and therefore are not curated

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Ochrobactrum daejeonense strain JCM 16234

Ochrobactrum daejeonense strain DSM 26944

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