Strain identifier

BacDive ID: 2268

Type strain: Yes

Species: Carnobacterium inhibens

Strain Designation: K1

Strain history: CIP <- 2000, CCUG <- 1993, A. Joborn, Univ. Göteborg, Göteborg, Sweden

NCBI tax ID(s): 1449339 (strain), 147709 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 4876

BacDive-ID: 2268

DSM-Number: 13024

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, motile, rod-shaped

description: Carnobacterium inhibens K1 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from gastrointestinal tract of Atlantic salmon .

NCBI tax id

NCBI tax idMatching level
1449339strain
147709species

strain history

@refhistory
4876<- S. Kjelleberg <- A. Jöborn; K1
67770CIP 106863 <-- CCUG 31728 <-- A. Jöborn K1.
120117CIP <- 2000, CCUG <- 1993, A. Joborn, Univ. Göteborg, Göteborg, Sweden

doi: 10.13145/bacdive2268.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Carnobacteriaceae
  • genus: Carnobacterium
  • species: Carnobacterium inhibens
  • full scientific name: Carnobacterium inhibens Jöborn et al. 1999

@ref: 4876

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Carnobacteriaceae

genus: Carnobacterium

species: Carnobacterium inhibens subsp. inhibens

full scientific name: Carnobacterium inhibens subsp. inhibens (Jöborn et al. 1999) Nicholson et al. 2015

strain designation: K1

type strain: yes

Morphology

cell morphology

  • @ref: 120117
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 120117
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4876COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
38657MEDIUM 45 - for Columbia agar with sheep bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Sheepblood (100.000 ml)
120117CIP Medium 45yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45

culture temp

@refgrowthtypetemperaturerange
4876positivegrowth30mesophilic
38657positivegrowth37mesophilic
67770positivegrowth37mesophilic
120117positivegrowth15-37
120117nogrowth5psychrophilic
120117nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4876microaerophile
120117facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
120117NaClpositivegrowth0-6 %
120117NaClnogrowth8 %
120117NaClnogrowth10 %

murein

  • @ref: 4876
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1201174853esculin+hydrolysis
120117606565hippurate+hydrolysis
12011717632nitrate-builds gas from
12011717632nitrate-reduction
12011716301nitrite+builds gas from
12011716301nitrite-reduction
6838129016arginine-hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose+builds acid from
6838116634raffinose-builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate+hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside+builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
12011735581indoleno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin-
12011715688acetoin-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
120117oxidase-
120117beta-galactosidase-3.2.1.23
120117alcohol dehydrogenase-1.1.1.1
120117gelatinase-
120117amylase-
120117DNase-
120117caseinase-3.4.21.50
120117catalase-1.11.1.6
120117tween esterase-
120117gamma-glutamyltransferase+2.3.2.2
120117lecithinase-
120117lipase-
120117lysine decarboxylase-4.1.1.18
120117ornithine decarboxylase-4.1.1.17
120117phenylalanine ammonia-lyase-4.3.1.24
120117protease-
120117tryptophan deaminase-
120117urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120117--++-----+++--------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
4876-+----++--+-+---------+--+--+---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120117------------------+--------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinentgeographic location
4876gastrointestinal tract of Atlantic salmon (Salmo salar)Salmo salarSwedenSWEEurope
50699Salmon gut,Salmo salarSwedenSWEEuropeGöteborg
67770Intestine of Atlantic salmon
120117Animal, Salmon, gutSwedenSWEEuropeGöteborg

isolation source categories

Cat1Cat2Cat3
#Host#Fishes#Salmonidae
#Host Body-Site#Gastrointestinal tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_5325.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_41;97_43;98_47;99_5325&stattab=map
  • Last taxonomy: Carnobacterium
  • 16S sequence: Z73313
  • Sequence Identity:
  • Total samples: 2245
  • soil counts: 136
  • aquatic counts: 366
  • animal counts: 1670
  • plant counts: 73

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
48761Risk group (German classification)
1201171Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Carnobacterium inhibens large 16S-23S rRNA intergenic spacer, tRNA-Ile and tRNA-Ala genes, complete sequenceAY170837445ena147709
20218Carnobacterium inhibens medium 16S-23S rRNA intergenic spacer and tRNA-Ala gene, complete sequenceAY170838325ena147709
20218Carnobacterium inhibens small 16S-23S rRNA intergenic spacer, complete sequenceAY170839217ena147709
4876Carnobacterium inhibens subsp. inhibens DSM 13024 16S rRNA gene, type strain K1TZ733131524ena1449339
67770Carnobacterium inhibens gene for 16S ribosomal RNA, partial sequence, strain: JCM 16168LC2581601459ena147709

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Carnobacterium inhibens subsp. inhibens DSM 13024 K1GCA_000746825contigncbi1449339
66792Carnobacterium inhibens DSM 13024 strain K11449339.3wgspatric1449339
66792Carnobacterium inhibens K1, DSM 130242574179783draftimg1449339

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno64.475no
gram-positiveyes92.749no
anaerobicno95.966yes
halophileyes78.155no
spore-formingno72.962no
thermophileno99.295yes
glucose-utilyes90.179no
aerobicno93.899yes
flagellatedno83.194no
glucose-fermentyes75.983no

External links

@ref: 4876

culture collection no.: DSM 13024, CCUG 31728, CIP 106863, JCM 12490, JCM 16168, CCM 7006, CECT 5963

straininfo link

  • @ref: 71826
  • straininfo: 49389

literature

  • topic: Phylogeny
  • Pubmed-ID: 10555373
  • title: Carnobacterium inhibens sp. nov., isolated from the intestine of Atlantic salmon (Salmo salar).
  • authors: Joborn A, Dorsch M, Olsson JC, Westerdahl A, Kjelleberg S
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-49-4-1891
  • year: 1999
  • mesh: Aeromonas/growth & development, Animals, Bacterial Typing Techniques, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Intestines/*microbiology, Lactobacillaceae/*classification/genetics/isolation & purification/physiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Salmo salar/*microbiology, Sequence Analysis, DNA, Vibrio/growth & development
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4876Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13024)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13024
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38657Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19040
50699Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31728)https://www.ccug.se/strain?id=31728
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71826Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49389.1StrainInfo: A central database for resolving microbial strain identifiers
120117Curators of the CIPCollection of Institut Pasteur (CIP 106863)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106863