Strain identifier

BacDive ID: 2253

Type strain: Yes

Species: Carnobacterium divergens

Strain Designation: 66

Culture col. no.: DSM 20623, ATCC 35677, NCDO 2763, WDCM 00075, CCUG 30094, CCM 4117

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8971

BacDive-ID: 2253

DSM-Number: 20623

keywords: microaerophile, Bacteria, 16S sequence, genome sequence, mesophilic

description: Carnobacterium divergens 66 is a microaerophile, mesophilic bacterium that was isolated from vacuum-packaged minced beef.

strain history: <- W.H. Holzapfel, 66

doi: 10.13145/bacdive2253.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/firmicutes
  • domain: Bacteria
  • phylum: Firmicutes
  • class: Bacilli
  • order: Lactobacillales
  • family: Carnobacteriaceae
  • genus: Carnobacterium
  • species: Carnobacterium divergens
  • full scientific name: Carnobacterium divergens (Holzapfel and Gerber 1984) Collins et al. 1987
  • synonyms

    • @ref: 20215
    • synonym: Lactobacillus divergens

@ref: 8971

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Carnobacteriaceae

genus: Carnobacterium

species: Carnobacterium divergens

full scientific name: Carnobacterium divergens (Holzapfel and Gerber 1984) Collins et al. 1987

strain designation: 66

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8971TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92), microaerobicyeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium92.pdf
41662MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)

culture temp

@refgrowthtypetemperaturerange
8971positivegrowth30mesophilic
41662positivegrowth30mesophilic
49897positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 49897
  • oxygen tolerance: microaerophile

murein

  • @ref: 8971
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8971-----+-----+++--------+++++++--++-+----+----------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
8971vacuum-packaged minced beef
49897Vacuum-packaged minced beefSouth AfricaZAFAfrica

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Bottled, canned, packed food
#Engineered#Food production#Meat

Safety information

risk assessment

  • @ref: 8971
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Carnobacterium divergens strain ATCC 35677 16S ribosomal RNA gene, partial sequenceGU460377809ena2748
20218Carnobacterium divergens strain DSM 20623 16S ribosomal RNA gene, partial sequenceM588161502ena2748
20218Carnobacterium divergens gene for 16S rRNA, partial sequence, strain: NBRC 15683AB6809401481ena2748
20218Carnobacterium divergens 16S ribosomal RNA gene, partial sequence; small 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF374292446ena2748
20218Carnobacterium divergens 16S ribosomal RNA gene, partial sequence; medium 16S-23S intergenic spacer and tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF374293554ena2748
20218Carnobacterium divergens 16S ribosomal RNA gene, partial sequence; large 16S-23S intergenic spacer, tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF374294668ena2748
20218Carnobacterium divergens partial 16S RNAX542701492ena2748

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Carnobacterium divergens DSM 20623 66GCA_000744255contigncbi1449336
66792Carnobacterium divergens TMW 2.1579GCA_001703555completencbi2748
66792Carnobacterium divergens MFPA43A1505GCA_900258435chromosomencbi2748
66792Carnobacterium divergens NCTC13772GCA_900446755contigncbi2748
66792Carnobacterium divergens DSM 20623GCA_001437085scaffoldncbi1449336
66792Carnobacterium divergens TMW 2.1577GCA_009295565contigncbi2748
66792Carnobacterium divergens C13GCA_004564395contigncbi2748
66792Carnobacterium divergens A10GCA_004564835contigncbi2748
66792Carnobacterium divergens C16GCA_004564415contigncbi2748
66792Carnobacterium divergens C6GCA_004564475contigncbi2748
66792Carnobacterium divergens B8GCA_004564535contigncbi2748
66792Carnobacterium divergens B7GCA_004564555contigncbi2748
66792Carnobacterium divergens CDIV41GCA_900088465contigncbi2748
66792Carnobacterium divergens A4GCA_004564915contigncbi2748
66792Carnobacterium divergens A12GCA_004564775contigncbi2748
66792Carnobacterium divergens A11GCA_004564785contigncbi2748
66792Carnobacterium divergens A2GCA_004564935contigncbi2748
66792Carnobacterium divergens A8GCA_004564865contigncbi2748
66792Carnobacterium divergens C10GCA_004565065contigncbi2748
66792Carnobacterium divergens C7GCA_004564425contigncbi2748
66792Carnobacterium divergens C5GCA_004564525contigncbi2748
66792Carnobacterium divergens B2GCA_004564655contigncbi2748
66792Carnobacterium divergens B1GCA_004564685contigncbi2748
66792Carnobacterium divergens B6GCA_004565125contigncbi2748
66792Carnobacterium divergens C14GCA_004565005contigncbi2748
66792Carnobacterium divergens A13GCA_004564765contigncbi2748
66792Carnobacterium divergens A9GCA_004565205contigncbi2748
66792Carnobacterium divergens C3GCA_004564515contigncbi2748
66792Carnobacterium divergens C15GCA_004564995contigncbi2748
66792Carnobacterium divergens C11GCA_004564435contigncbi2748
66792Carnobacterium divergens B5GCA_004564605contigncbi2748
66792Carnobacterium divergens C4GCA_004565095contigncbi2748
66792Carnobacterium divergens C9GCA_004565085contigncbi2748
66792Carnobacterium divergens C8GCA_004564405contigncbi2748
66792Carnobacterium divergens C1GCA_004564565contigncbi2748
66792Carnobacterium divergens B4GCA_004564615contigncbi2748
66792Carnobacterium divergens B3GCA_004564645contigncbi2748
66792Carnobacterium divergens C17GCA_004564965contigncbi2748
66792Carnobacterium divergens C18GCA_004564985contigncbi2748
66792Carnobacterium divergens C12GCA_004565035contigncbi2748
66792Carnobacterium divergens C2GCA_004565115contigncbi2748
66792Carnobacterium divergens DSM 206231449336.4wgspatric1449336
66792Carnobacterium divergens DSM 20623 strain 661449336.3wgspatric1449336
66792Carnobacterium divergens strain A102748.55wgspatric2748
66792Carnobacterium divergens strain A112748.54wgspatric2748
66792Carnobacterium divergens strain A122748.53wgspatric2748
66792Carnobacterium divergens strain A132748.52wgspatric2748
66792Carnobacterium divergens strain A22748.59wgspatric2748
66792Carnobacterium divergens strain A42748.56wgspatric2748
66792Carnobacterium divergens strain A82748.57wgspatric2748
66792Carnobacterium divergens strain A92748.68wgspatric2748
66792Carnobacterium divergens strain B12748.51wgspatric2748
66792Carnobacterium divergens strain B22748.50wgspatric2748
66792Carnobacterium divergens strain B32748.49wgspatric2748
66792Carnobacterium divergens strain B42748.48wgspatric2748
66792Carnobacterium divergens strain B52748.47wgspatric2748
66792Carnobacterium divergens strain B62748.69wgspatric2748
66792Carnobacterium divergens strain B72748.45wgspatric2748
66792Carnobacterium divergens strain B82748.44wgspatric2748
66792Carnobacterium divergens strain C12748.46wgspatric2748
66792Carnobacterium divergens strain C102748.64wgspatric2748
66792Carnobacterium divergens strain C112748.40wgspatric2748
66792Carnobacterium divergens strain C122748.63wgspatric2748
66792Carnobacterium divergens strain C132748.36wgspatric2748
66792Carnobacterium divergens strain C142748.62wgspatric2748
66792Carnobacterium divergens strain C152748.60wgspatric2748
66792Carnobacterium divergens strain C162748.38wgspatric2748
66792Carnobacterium divergens strain C172748.58wgspatric2748
66792Carnobacterium divergens strain C182748.61wgspatric2748
66792Carnobacterium divergens strain C22748.67wgspatric2748
66792Carnobacterium divergens strain C32748.42wgspatric2748
66792Carnobacterium divergens strain C42748.66wgspatric2748
66792Carnobacterium divergens strain C52748.43wgspatric2748
66792Carnobacterium divergens strain C62748.41wgspatric2748
66792Carnobacterium divergens strain C72748.39wgspatric2748
66792Carnobacterium divergens strain C82748.37wgspatric2748
66792Carnobacterium divergens strain C92748.65wgspatric2748
66792Carnobacterium divergens strain CDIV412748.5wgspatric2748
66792Carnobacterium divergens strain NCTC137722748.19wgspatric2748
66792Carnobacterium divergens strain TMW 2.15772748.70wgspatric2748
66792Carnobacterium divergens strain TMW 2.15792748.21completepatric2748
66792Carnobacterium divergens 662574180433permanentimg2748

GC content

  • @ref: 8971
  • GC-content: 35.5

External links

@ref: 8971

culture collection no.: DSM 20623, ATCC 35677, NCDO 2763, WDCM 00075, CCUG 30094, CCM 4117

straininfo link

@refpassport
20218http://www.straininfo.net/strains/6789
20218http://www.straininfo.net/strains/6786
20218http://www.straininfo.net/strains/120951
20218http://www.straininfo.net/strains/866272

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8971Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20623)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20623
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41662Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/12559
49897Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 30094)https://www.ccug.se/strain?id=30094
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)