Strain identifier

BacDive ID: 222

Type strain: Yes

Species: Aerococcus urinaeequi

Strain Designation: PUE

Strain history: CIP 103442 <-- NCDO 1636 <-- Tech. Hogesch., Delft <-- R. H. Mees PUE.

NCBI tax ID(s): 1120952 (strain), 51665 (species)

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General

@ref: 8746

BacDive-ID: 222

DSM-Number: 20341

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, sphere-shaped

description: Aerococcus urinaeequi PUE is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from horse urine.

NCBI tax id

NCBI tax idMatching level
51665species
1120952strain

strain history

@refhistory
8746<- NCDO <- Techn. Hoogeschool Delft <- R.H. Mees, PUE
67770CIP 103442 <-- NCDO 1636 <-- Tech. Hogesch., Delft <-- R. H. Mees PUE.

doi: 10.13145/bacdive222.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Aerococcaceae
  • genus: Aerococcus
  • species: Aerococcus urinaeequi
  • full scientific name: Aerococcus urinaeequi (Garvie 1988 ex Mees 1934) Felis et al. 2005

  • synonyms

    • @ref: 20215
    • synonym: Pediococcus urinaeequi

@ref: 8746

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Aerococcaceae

genus: Aerococcus

species: Aerococcus urinaeequi

full scientific name: Aerococcus urinaeequi (Garvie 1988) Felis et al. 2005 emend. Tohno et al. 2014

strain designation: PUE

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
22981positivesphere-shapedno
69480no97.002
69480positive100

colony morphology

  • @ref: 49095
  • incubation period: 1 day

Culture and growth conditions

culture medium

  • @ref: 8746
  • name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/92
  • composition: Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
8746positivegrowth30mesophilic
22981positiveoptimum25.0-30.0mesophilic
49095positivegrowth37mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
22981positiveoptimum8.5-9.0alkaliphile
22981positiveminimum6.5-7.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
22981facultative anaerobe
49095microaerophile

spore formation

@refspore formationconfidence
22981no
69481no100
69480no99.998

murein

  • @ref: 8746
  • murein short key: A11
  • type: A1alpha L-Lys-direct

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2298117754glycerol-builds acid from
229816731melezitose-builds acid from
2298130911sorbitol-builds acid from
2298128017starch-builds acid from
2298123652dextrin+builds acid from
2298117306maltose+builds acid from
2298117992sucrose+builds acid from
2298127082trehalose+builds acid from
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose+builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

enzymes

  • @ref: 22981
  • value: catalase
  • activity: -
  • ec: 1.11.1.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    49095C14:05.814
    49095C16:022.516
    49095C18:06.818
    49095C16:1 ω7c/C15:0 ISO 2OH2.315.85
    49095C18:1 ω9c28.417.769
    49095C18:2 ω6,9c/C18:0 ANTE4.317.724
    49095Unidentified113.775
    49095Unidentified1.413.863
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8746----------++++----+----+++++++-++--+---+-------+/---

Isolation, sampling and environmental information

isolation

@refsample type
8746horse urine
49095Horse urine
67770Horse, urine

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Equidae (Horse)
#Host Body Product#Fluids#Urine

taxonmaps

  • @ref: 69479
  • File name: preview.99_1645.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_938;97_1091;98_1301;99_1645&stattab=map
  • Last taxonomy: Aerococcus
  • 16S sequence: LC153547
  • Sequence Identity:
  • Total samples: 116598
  • soil counts: 8338
  • aquatic counts: 13880
  • animal counts: 90338
  • plant counts: 4042

Safety information

risk assessment

  • @ref: 8746
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Pediococcus urinaeequi ATCC 29723 16S ribosomal RNA gene, partial sequenceAF429541514ena51665
20218Pediococcus urinaeequi strain ATCC 29723 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF429628539ena51665
8746Pediococcus urinaeequi DNA for 16S rRNA, partial sequenceD876771419ena51665
67770Aerococcus urinaeequi gene for 16S ribosomal RNA, partial sequence, strain: JCM 12519LC1535471372ena51665

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aerococcus urinaeequi CCUG28094GCA_001543205completencbi51665
66792Aerococcus urinaeequi DSM 203411120952.3wgspatric1120952
66792Aerococcus urinaeequi strain CCUG2809451665.4completepatric51665
66792Aerococcus urinaeequi CCUG280942671180343completeimg51665
66792Aerococcus urinaeequi DSM 203412524614650draftimg1120952
67770Aerococcus urinaeequi DSM 20341 = CCUG 28094GCA_000425085scaffoldncbi1120952

GC content

@refGC-contentmethod
874639.5
874640.0high performance liquid chromatography (HPLC)
6777040high performance liquid chromatography (HPLC)
6777039.5high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno96.919yes
gram-positiveyes95.717yes
anaerobicno94.47yes
aerobicno93.449no
halophileyes92.228no
spore-formingno96.44yes
thermophileno99.149no
glucose-utilyes90.199no
motileno97.559yes
glucose-fermentyes81.257no

External links

@ref: 8746

culture collection no.: CCUG 28094, CIP 103442, NCIMB 701636, DSM 20341, ATCC 29723, JCM 12519, NCDO 1636, LMG 11420, LMG 13989

straininfo link

  • @ref: 69905
  • straininfo: 3825

literature

topicPubmed-IDtitleauthorsjournalDOIyear
Genetics27103727Complete Genome Sequences of Aerococcus christensenii CCUG 28831T, Aerococcus sanguinicola CCUG 43001T, Aerococcus urinae CCUG 36881T, Aerococcus urinaeequi CCUG 28094T, Aerococcus urinaehominis CCUG 42038 BT, and Aerococcus viridans CCUG 4311T.Carkaci D, Dargis R, Nielsen XC, Skovgaard O, Fuursted K, Christensen JJGenome Announc10.1128/genomeA.00302-162016
Genetics35477007Identification of an Aerococcus urinaeequi isolate by whole genome sequencing and average nucleotide identity analysis.Zhou W, Gao S, Zheng J, Zhang Y, Zhou H, Zhang Z, Cao X, Shen HJ Glob Antimicrob Resist10.1016/j.jgar.2022.04.0132022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8746Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20341)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20341
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22981Giovanna E. Felis,Sandra Torriani,Franco Dellaglio10.1099/ijs.0.63324-0Reclassification of Pediococcus urinaeequi (ex Mees 1934) Garvie 1988 as Aerococcus urinaeequi comb. nov.IJSEM 55: 1325-1327 200515879276
49095Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 28094)https://www.ccug.se/strain?id=28094
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69905Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3825.1StrainInfo: A central database for resolving microbial strain identifiers