Strain identifier

BacDive ID: 2125

Type strain: Yes

Species: Campylobacter sputorum subsp. bubulus

Strain Designation: Paris, Waterleo

Strain history: CIP <- 1953, J. Gallut, Campylobacter sputorum subsp. bubulus <- A. Florent: strain Waterleo

NCBI tax ID(s): 206 (species), 32023 (subspecies)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 2003

BacDive-ID: 2125

DSM-Number: 5363

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, rod-shaped, animal pathogen

description: Campylobacter sputorum subsp. bubulus Paris is a microaerophile, mesophilic, rod-shaped animal pathogen that was isolated from Bull sperm.

NCBI tax id

NCBI tax idMatching level
32023subspecies
206species

strain history

@refhistory
2003<- CCUG; CCUG 11289 <- CIP; CIP 53.103 <- A. Florent;
67771<- CCUG <- CIP, Paris, France <- J Gallut,1953 <- A Florent, Waterleo
123287CIP <- 1953, J. Gallut, Campylobacter sputorum subsp. bubulus <- A. Florent: strain Waterleo

doi: 10.13145/bacdive2125.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Epsilonproteobacteria
  • order: Campylobacterales
  • family: Campylobacteraceae
  • genus: Campylobacter
  • species: Campylobacter sputorum subsp. bubulus
  • full scientific name: Campylobacter sputorum subsp. bubulus (Florent 1953) Véron and Chatelain 1973 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Vibrio bubulus

@ref: 2003

domain: Bacteria

phylum: Proteobacteria

class: Epsilonproteobacteria

order: Campylobacterales

family: Campylobacteraceae

genus: Campylobacter

species: Campylobacter sputorum subsp. bubulus

full scientific name: Campylobacter sputorum subsp. bubulus (Florent 1953) Véron and Chatelain 1973

strain designation: Paris, Waterleo

type strain: yes

Morphology

cell morphology

@refcell shapegram stainmotility
67771rod-shaped
67771negative
123287rod-shapednegativeyes

colony morphology

@refincubation period
454162-3 days
123287

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2003SEMISOLID BRUCELLA BROTH (DSMZ Medium 703)yeshttps://mediadive.dsmz.de/medium/703Name: SEMISOLID BRUCELLA BROTH (DSMZ Medium 703) Composition: Casein peptone 10.0 g/l Bacto peptone 10.0 g/l NaCl 5.0 g/l Yeast extract 2.0 g/l Agar 1.6 g/l D-Glucose 1.0 g/l Distilled water
35166MEDIUM 262 - Columbia agar with 30 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (300.000 ml)
123287Brucella brothyes
123287CIP Medium 262yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=262
123287CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
2003positivegrowth37mesophilic
35166positivegrowth37mesophilic
45416positivegrowth37mesophilic
45416positivegrowth42thermophilic
67771positivegrowth37mesophilic
123287positivegrowth25-41
123287nogrowth5psychrophilic
123287nogrowth10psychrophilic
123287nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
2003microaerophile
45416microaerophile
67771microaerophile
123287microaerophile

halophily

  • @ref: 123287
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 3.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
123287606565hippurate-hydrolysis
12328717632nitrate+reduction
12328716301nitrite-reduction
6837316199urea-hydrolysis
6837317632nitrate+reduction
6837317634D-glucose-assimilation
6837317272propionate-assimilation
6837316947citrate-assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
6837348923erythromycinyesyes14 µg
68373474053cefazolinyesyes224 µg
68373100147nalidixic acidyesyes84 µg

metabolite production

  • @ref: 123287
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
2003catalase-1.11.1.6
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68373alkaline phosphatase-3.1.3.1
68373L-arginine arylamidase-
68373pyrrolidonyl arylamidase-3.4.19.3
68373gamma-glutamyltransferase-2.3.2.2
68373urease-3.5.1.5
123287oxidase+
123287gelatinase-
123287DNase-
123287catalase-1.11.1.6
123287gamma-glutamyltransferase-2.3.2.2
123287urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    45416C12:00.812
    45416C14:021.614
    45416C16:024.916
    45416C12:0 ALDE ?2.810.915
    45416C13:0 ISO 2OH0.813.814
    45416C13:1 at 12-133.212.931
    45416C14:0 3OH/C16:1 ISO I2.815.485
    45416C16:0 3OH1.217.52
    45416C16:1 ω7c9.615.819
    45416C18:1 ω7c /12t/9t30.517.824
    45416C18:2 ω6,9c/C18:0 ANTE1.917.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123287--++------+----+----

API CAM

@refURENITESTHIPGGTTTCPYRAArgAAspAPALH2SGLUSUTNALCFZACEPROPMLTCITEROCAT
2003-+------+---+--------
45416-++--+--+-+-+--+-+---
2003-+--------+-+/--------+
2003-+-+------+----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
45416Bull spermBrussels [6527]BelgiumBELEurope
2003bull sperm
67771From mammal, bull sperm
123287Animal, Bull, sperm1953

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body Product#Urogenital tract#Semen

taxonmaps

  • @ref: 69479
  • File name: preview.99_28210.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15775;96_2981;97_3665;98_4637;99_28210&stattab=map
  • Last taxonomy: Campylobacter sputorum subclade
  • 16S sequence: NR_043600
  • Sequence Identity:
  • Total samples: 328
  • soil counts: 5
  • aquatic counts: 1
  • animal counts: 322

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
2003yes2Risk group (German classification)
1232872Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
67771Campylobacter sputorum subsp. bubulus strain NCTC 11367 16S ribosomal RNA (rrs) gene, partial sequenceDQ1741511570ena32023
67771Campylobacter sputorum subsp. bubulus strain NCTC 11367 16S ribosomal RNA, partial sequenceNR_0436001570nuccore32023
67771C.sputorum bubulus 23S rRNA geneX67772780ena206

GC content

  • @ref: 2003
  • GC-content: 29.9
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 2003

culture collection no.: DSM 5363, ATCC 33562, CCUG 11289, CIP 53.103, LMG 6447, NCTC 11367, CIP 53103, KCTC 15210

Reference

@idauthorscataloguedoi/urltitle
2003Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 5363)https://www.dsmz.de/collection/catalogue/details/culture/DSM-5363
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
35166Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16411
45416Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 11289)https://www.ccug.se/strain?id=11289
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68373Automatically annotated from API CAM
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
123287Curators of the CIPCollection of Institut Pasteur (CIP 53.103)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2053.103