Name: Campylobacter sputorum subsp. bubulus Paris
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2125

Type strain:

Species: Campylobacter sputorum subsp. bubulus

Strain Designation: Paris, Waterleo

Culture col. no.: DSM 5363, ATCC 33562, CCUG 11289, CIP 53.103, LMG 6447, NCTC 11367, CIP 53103, KCTC 15210

Strain history: CIP <- 1953, J. Gallut, Campylobacter sputorum subsp. bubulus <- A. Florent: strain Waterleo

NCBI tax ID(s): 206 (species), 32023 (subspecies)

Other strains from Campylobacter sputorum subsp. bubulus

C. sputorum subsp. bubulus CCUG 886, NCTC 10355
C. sputorum subsp. bubulus CCUG 24262

Section

Campylobacter sputorum subsp. bubulus Paris is a microaerophile, mesophilic, rod-shaped animal pathogen that was isolated from Bull sperm.

rod-shaped
animal pathogen
mesophilic
microaerophile
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Epsilonproteobacteria*
	Order	*Campylobacterales*
	Family	*Campylobacteraceae*
	Genus	*Campylobacter*
	Species	**Campylobacter sputorum subsp. bubulus**
	Full Scientific Name (LPSN)	Campylobacter sputorum subsp. bubulus (Florent 1953) Véron and Chatelain 1973 (Approved Lists 1980)

Synonym

"Vibrio bubulus"

Information on morphological and physiological properties Morphology

[Ref.: #67771]	Gram stain	negative
[Ref.: #123287]	Gram stain	negative

[Ref.: #123287]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #123287]	Motility	yes

[Ref.: #45416]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2003]

Culture medium

SEMISOLID BRUCELLA BROTH (DSMZ Medium 703)

[Ref.: #2003]

Culture medium growth

[Ref.: #2003]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2003]

Culture medium composition

expand / minimize

[Ref.: #35166]

Culture medium

MEDIUM 262 - Columbia agar with 30 % horse blood

[Ref.: #35166]

Culture medium growth

[Ref.: #35166]

Culture medium composition

expand / minimize

[Ref.: #123287]

Culture medium

Brucella broth

[Ref.: #123287]

Culture medium growth

[Ref.: #123287]

Culture medium

CIP Medium 262

[Ref.: #123287]

Culture medium growth

[Ref.: #123287]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123287]

Culture medium

CIP Medium 72

[Ref.: #123287]

Culture medium growth

[Ref.: #123287]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2003]		growth	37 °C
[Ref.: #35166]		growth	37 °C
[Ref.: #45416]		growth	37 °C
[Ref.: #45416]		growth	42 °C
[Ref.: #67771]		growth	37 °C
[Ref.: #123287]		growth	25-41 °C
[Ref.: #123287]	no	growth	5 °C
[Ref.: #123287]	no	growth	10 °C
[Ref.: #123287]	no	growth	45 °C

[Ref.: #2003]	Temperature range	mesophilic
[Ref.: #35166]	Temperature range	mesophilic
[Ref.: #45416]	Temperature range	mesophilic
[Ref.: #45416]	Temperature range	thermophilic
[Ref.: #67771]	Temperature range	mesophilic
[Ref.: #123287]	Temperature range	psychrophilic
[Ref.: #123287]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #2003]	Oxygen tolerance	microaerophile
[Ref.: #45416]	Oxygen tolerance	microaerophile
[Ref.: #67771]	Oxygen tolerance	microaerophile
[Ref.: #123287]	Oxygen tolerance	microaerophile

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #123287]		NaCl		growth	3.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68373]	citrate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	D-glucose ChEBI	-	assimilation	* from API CAM
[Ref.: #123287]	hippurate ChEBI	-	hydrolysis
[Ref.: #123287]	nitrate ChEBI	+	reduction
[Ref.: #68373]	nitrate ChEBI	+	reduction	* from API CAM
[Ref.: #123287]	nitrite ChEBI	-	reduction
[Ref.: #68373]	propionate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	urea ChEBI	-	hydrolysis	* from API CAM

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #68373]	cefazolin ChEBI	yes	224	µg	* from API CAM
[Ref.: #68373]	erythromycin ChEBI	yes	14	µg	* from API CAM
[Ref.: #68373]	nalidixic acid ChEBI	yes	84	µg	* from API CAM

	Metabolite production	Metabolite	Production
[Ref.: #123287]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API CAM
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #2003]	catalase	-	1.11.1.6
[Ref.: #123287]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #123287]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #123287]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #68373]	gamma-glutamyltransferase	-	2.3.2.2	* from API CAM
[Ref.: #123287]	gelatinase	-
[Ref.: #68373]	L-arginine arylamidase	-		* from API CAM
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #123287]	oxidase	+
[Ref.: #68373]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API CAM
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123287]	urease	-	3.5.1.5
[Ref.: #68373]	urease	-	3.5.1.5	* from API CAM
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #45416]

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	30.5	17.824
C16:0	24.9	16
C14:0	21.6	14
C16:1 ω7c	9.6	15.819
C13:1 at 12-13	3.2	12.931
C12:0 ALDE ?	2.8	10.915
C14:0 3OH/C16:1 ISO I	2.8	15.485
C18:2 ω6,9c/C18:0 ANTE	1.9	17.724
C16:0 3OH	1.2	17.52
C13:0 ISO 2OH	0.8	13.814
C12:0	0.8	12

API® CAM:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Reduction of nitrateNIT	EsteraseEST	Hippuric acidHIP	gamma-Glutamyl transferaseGGT	Reduction of Triphenyl tetrazolium chlorideTTC	Pyrrolidonyl arylamidasePYRA	L-arginine arylamidaseArgA	L-aspartic acid arylamidaseAspA	Alkaline phosphatasePAL	H2S productionH2S	Assimilation of D-glucoseGLU	Assimilation of succinateSUT	Growth inhibitionNAL	Growth inhibitionCFZ	Assimilation of acetateACE	Assimilation of propionic acidPROP	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Erythromycin resistance (+) sensitivity (-)ERO	CatalaseCAT
[Ref.: #2003]	3923	-	+	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-
[Ref.: #45416]	1559	-	+	+	-	-	+	-	-	+	-	+	-	+	-	-	+	-	+	-	-	-
[Ref.: #2003]	3684	-	+	-	-	-	-	-	-	-	-	+	-	+/-	-	-	-	-	-	-	-	+
[Ref.: #2003]	3683	-	+	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123287]	12682	-	-	+	+	-	-	-	-	-	-	+	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #45416]	Sample type/isolated from	Bull sperm
[Ref.: #45416]	Geographic location (country and/or sea, region)	Brussels [6527]
[Ref.: #45416]	Country	Belgium
[Ref.: #45416]	Country ISO 3 Code	BEL
[Ref.: #45416]	Continent	Europe

[Ref.: #2003]	Sample type/isolated from	bull sperm

[Ref.: #67771]	Sample type/isolated from	From mammal, bull sperm

[Ref.: #123287]	Sample type/isolated from	Animal, Bull, sperm
[Ref.: #123287]	Isolation date	1953
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Urogenital tract	#Semen

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence NR_043600 (>99% sequence identity) for Campylobacter sputorum subclade from Microbeatlas

Aquatic Samples	1
Soil Samples	5
Animal Samples	322
Plant Samples
Total Samples	328

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2003]	Pathogenicity (animal)	yes
[Ref.: #2003]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #123287]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67771]	Campylobacter sputorum subsp. bubulus strain NCTC 11367 16S ribosomal RNA (rrs) gene, partial sequence	DQ174151	1570	ENA	32023 tax ID
[Ref.: #67771]	Campylobacter sputorum subsp. bubulus strain NCTC 11367 16S ribosomal RNA, partial sequence	NR_043600	1570	GenBank	32023 tax ID
[Ref.: #67771]	C.sputorum bubulus 23S rRNA gene	X67772	780	ENA	206 tax ID

[Ref.: #2003]

GC-content

29.9 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #2003]

Culture collection no.

DSM 5363, ATCC 33562, CCUG 11289, CIP 53.103, LMG 6447, NCTC 11367, CIP 53103, KCTC 15210

References

#2003

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5363

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#35166 ; Curators of the CIP;
#45416 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 11289
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68373

Automatically annotated from API CAM .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#123287 Collection of Institut Pasteur ; Curators of the CIP; CIP 53.103
* These data were automatically processed and therefore are not curated

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