Strain identifier
BacDive ID: 20893
Type strain: 
Species: Nannocystis exedens
Strain Designation: Na e619
NCBI tax ID(s): 54 (species)
version 8.1 (current version)
General
@ref: 18281
BacDive-ID: 20893
keywords: Bacteria
description: Nannocystis exedens Na e619 is a bacterium that builds a fruiting body and was isolated from soil with plant residues.
NCBI tax id
- NCBI tax id: 54
- Matching level: species
doi: 10.13145/bacdive20893.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Deltaproteobacteria
- order: Myxococcales
- family: Nannocystaceae
- genus: Nannocystis
- species: Nannocystis exedens
- full scientific name: Nannocystis exedens Reichenbach 1970 (Approved Lists 1980)
@ref: 18281
domain: Bacteria
phylum: Proteobacteria
class: Deltaproteobacteria
order: Myxococcales
family: Nannocystaceae
genus: Nannocystis
species: Nannocystis exedens
full scientific name: Nannocystis exedens
strain designation: Na e619
type strain: no
Morphology
multicellular morphology
- @ref: 18281
- forms multicellular complex: yes
- complex name: fruiting body
Culture and growth conditions
culture medium
| @ref | name | growth | composition |
|---|---|---|---|
| 18281 | CA2 AGAR | yes | Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g Basal medium: Agar 1.5% Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements |
| 18281 | CHITIN 7 AGAR | yes | Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates |
| 18281 | COLE AGAR | yes | Ingredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells |
| 18281 | KAN 1 AGAR | no | Ingredients: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% CaCl2 x 2H2O 0.02% MgSO4 x 7H2O 0.05% Agar 1.5% KNO3 0.1% Preparation / Remarks: pH 7.2 after autoclaving add: glucose 0.2% (autoclaved separately) 50 mg/L Kanamycin-sulfate (sterilized by filtration) |
| 18281 | KAN 2 AGAR | yes | Ingredients: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% CaCl2 x 2H2O 0.02% MgSO4 x 7H2O 0.05% Agar 1.5% KNO3 0.1% Kanamycin-sulfate 100 mg/L Preparation / Remarks: pH 7.2 after autoclaving add: Glucose 0.2% (autoclaved separately) 100 mg/L Kanamycin-sulfate (sterilized by filtration) |
| 18281 | KAN 3 AGAR | yes | Ingredients: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% CaCl2 x 2H2O 0.02% MgSO4 x 7H2O 0.05% Agar 1.5% KNO3 0.1% Preparation / Remarks: pH 7.2 after autoclaving add: Glucose 0.2% (autoclaved separately) 250 mg/L Kanamycin-sulfate (sterilized by filtration) |
| 18281 | Myx MEDIUM | yes | Ingredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416. |
| 18281 | PROBION L AGAR | yes | Ingredients: Probion L 600 (Hoechst) 0.8% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 autoclaved |
| 18281 | SKIM MILK 2 AGAR | no | Ingredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on CA2-plates. |
| 18281 | SKIM MILK 3 AGAR | no | Ingredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on CYG2-plates. |
| 18281 | TOLI AGAR | yes | Ingredients: CaCl2 x 2 H2O1.3g Agar (Difco)15.0g Distilled water1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak autoclaved E.coli cells |
| 18281 | VY/2 AGAR | yes | Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved |
Physiology and metabolism
observation
| @ref | observation |
|---|---|
| 18281 | Strong ability to Yeast lysis |
| 18281 | Ability for casein degradation |
| 18281 | No ability for chitin degradation |
| 18281 | No ability for protein degradation |
| 18281 | Ability for Yeast lysis |
| 18281 | Ability for E. coli lysis |
antibiotic resistance
| @ref | ChEBI | metabolite | is antibiotic | is resistant | resistance conc. |
|---|---|---|---|---|---|
| 18281 | 6104 | kanamycin | yes | yes | 100 µg/mL |
| 18281 | 6104 | kanamycin | yes | yes | 250 µg/mL |
Isolation, sampling and environmental information
isolation
- @ref: 18281
- sample type: soil with plant residues
- sampling date: 1988-09-01
- geographic location: Mallorca Island
- country: Spain
- origin.country: ESP
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Soil |
| #Host | #Plants |
External links
@ref: 18281
Reference
| @id | authors | title | doi/url | journal | pubmed |
|---|---|---|---|---|---|
| 18281 | Reichenbach, H. | Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 |