Strain identifier

BacDive ID: 2083

Type strain: Yes

Species: Ralstonia solanacearum

Strain Designation: 60-1, K60

Strain history: LMG 2299 <-- NCPPB 325 <-- A. Kelman.

NCBI tax ID(s): 1091042 (strain), 305 (species)

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General

@ref: 3633

BacDive-ID: 2083

DSM-Number: 9544

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, plant pathogen

description: Ralstonia solanacearum 60-1 is an aerobe, mesophilic plant pathogen that was isolated from Lycopersicon esculentum.

NCBI tax id

NCBI tax idMatching level
305species
1091042strain

strain history

@refhistory
3633<- NCPPB <- A. Kelman; 60-1
67770LMG 2299 <-- NCPPB 325 <-- A. Kelman.

doi: 10.13145/bacdive2083.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Ralstonia
  • species: Ralstonia solanacearum
  • full scientific name: Ralstonia solanacearum (Smith 1896) Yabuuchi et al. 1996

  • synonyms

    @refsynonym
    20215Pseudomonas solanacearum
    20215Bacillus solanacearum
    20215Burkholderia solanacearum

@ref: 3633

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Ralstonia

species: Ralstonia solanacearum

full scientific name: Ralstonia solanacearum (Smith 1896) Yabuuchi et al. 1996 emend. Safni et al. 2014

strain designation: 60-1, K60

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 3633
  • name: NUTRIENT AGAR (DSMZ Medium 1)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1
  • composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
3633positivegrowth28mesophilic
46075positivegrowth28mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 46075
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
3633----+---+--------++-+/-

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinentgeographic location
3633Lycopersicon esculentumLycopersicon esculentumUSAUSANorth America
46075Lycopersicon esculentumUSAUSANorth America
67770Tomato (Lycopersicon esculentum)Lycopersicon esculentumUSAUSANorth AmericaNC

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Herbaceous plants (Grass,Crops)

taxonmaps

  • @ref: 69479
  • File name: preview.99_64.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_12;96_51;97_54;98_59;99_64&stattab=map
  • Last taxonomy: Ralstonia
  • 16S sequence: EF016361
  • Sequence Identity:
  • Total samples: 21037
  • soil counts: 2671
  • aquatic counts: 4060
  • animal counts: 7979
  • plant counts: 6327

Safety information

risk assessment

  • @ref: 3633
  • pathogenicity plant: yes
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Ralstonia solanacearum gene for 16S ribosomal RNA, 23S ribosomal RNA, partial sequenceD87086554ena1091042
20218P.solanacearum (ATCC 11696) 16S ribosomal RNAX670361470ena1091042
20218Ralstonia solanacearum strain LMG2299T 16S-23S ribosomal RNA intergenic spacer, partial sequenceKC756967501ena305
3633Ralstonia solanacearum strain LMG 2299 external transcribed spacer and 16S ribosomal RNA gene, partial sequenceEF0163611497ena1091042

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Ralstonia solanacearum NCPPB 325GCA_015910705contigncbi305
66792Ralstonia solanacearum K60GCA_002251695contigncbi1091042
66792Ralstonia solanacearum K60GCA_000285815contigncbi1091042
66792Ralstonia solanacearum strain NCPPB 325305.901wgspatric305
66792Ralstonia solanacearum K602537561801draftimg1091042

GC content

  • @ref: 3633
  • GC-content: 66.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 3633

culture collection no.: DSM 9544, ATCC 11696, ICMP 5712, ICPB PS 256, LMG 2299, NCPPB 325, CCUG 14272, JCM 10489, LMG 17137, UQRS 426, CIP 104762, NRRL B-3212

straininfo link

  • @ref: 71730
  • straininfo: 4245

literature

  • topic: Metabolism
  • Pubmed-ID: 22712584
  • title: Preventing Ralstonia solanacearum adhesion with glycans from cashew, cocoa, coffee, pumpkin, and tomato seed extract.
  • authors: Rachmaninov O, Zinger-Yosovich KD, Gilboa-Garber N
  • journal: Can J Microbiol
  • DOI: 10.1139/w2012-062
  • year: 2012
  • mesh: Anacardium/chemistry/metabolism, Blotting, Western, Cacao/chemistry/metabolism, Coffea/chemistry/metabolism, Cucurbita/chemistry/metabolism, Lectins/*metabolism, Lycopersicon esculentum/chemistry/metabolism, Mannose/analysis/metabolism, Polysaccharides/analysis/metabolism/*pharmacology, Protein Binding/drug effects, Ralstonia solanacearum/*drug effects/metabolism, Seeds/chemistry/metabolism
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3633Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9544)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9544
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
46075Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 14272)https://www.ccug.se/strain?id=14272
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71730Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID4245.1StrainInfo: A central database for resolving microbial strain identifiers