Name: Ralstonia solanacearum 60-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2083

Type strain:

Species: Ralstonia solanacearum

Strain Designation: 60-1, K60

Culture col. no.: DSM 9544, ATCC 11696, ICMP 5712, ICPB PS 256, LMG 2299, NCPPB 325, CCUG 14272, JCM 10489, LMG 17137, UQRS 426, CIP 104762, NRRL B-3212

Strain history: LMG 2299 <-- NCPPB 325 <-- A. Kelman.

NCBI tax ID(s): 1091042 (strain), 305 (species)

SI-ID 4245

LMG 2299, ATCC 11696, CCUG 14272, DSM 9544, ICMP 5712, JCM 10489, LMG 17137, NCPPB 325, PDDCC 5712, CIP 104762, CFBP 2047, NRRL B-3212, BCRC 12850, CCRC 12850, KACC 10814, KACC 10149, NCAIM B.01459, VTT E-991288

Other strains from Ralstonia solanacearum

Section

Ralstonia solanacearum 60-1 is an aerobe, mesophilic plant pathogen that was isolated from Lycopersicon esculentum.

aerobe
mesophilic
plant pathogen
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Ralstonia*
	Species	**Ralstonia solanacearum**
	Full Scientific Name (LPSN)	Ralstonia solanacearum (Smith 1896) Yabuuchi et al. 1996

	Synonym	Pseudomonas solanacearum
	Synonym	"Bacillus* solanacearum"*
	Synonym	Burkholderia solanacearum

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3633]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #3633]

Culture medium growth

[Ref.: #3633]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3633]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #3633]	growth	28 °C
[Ref.: #46075]	growth	28 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #3633]	Temperature range	mesophilic
[Ref.: #46075]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #46075]

Oxygen tolerance

aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	+	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	+	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #3633]	23181	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-	+	+	-	+/-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3633]	Sample type/isolated from	Lycopersicon esculentum
[Ref.: #3633]	Host species	Lycopersicon esculentum
[Ref.: #3633]	Country	USA
[Ref.: #3633]	Country ISO 3 Code	USA
[Ref.: #3633]	Continent	North America

[Ref.: #46075]	Sample type/isolated from	Lycopersicon esculentum
[Ref.: #46075]	Country	USA
[Ref.: #46075]	Country ISO 3 Code	USA
[Ref.: #46075]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Tomato (Lycopersicon esculentum)
[Ref.: #67770]	Host species	Lycopersicon esculentum
[Ref.: #67770]	Geographic location (country and/or sea, region)	NC
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host

#Plants

#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EF016361 (>99% sequence identity) for Ralstonia from Microbeatlas

Aquatic Samples	4060
Soil Samples	2671
Animal Samples	7979
Plant Samples	6327
Total Samples	21037

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3633]	Pathogenicity (plant)	yes
[Ref.: #3633]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3633]	Ralstonia solanacearum strain LMG 2299 external transcribed spacer and 16S ribosomal RNA gene, partial sequence	EF016361	1497	ENA	1091042 tax ID
[Ref.: #20218]	Ralstonia solanacearum gene for 16S ribosomal RNA, 23S ribosomal RNA, partial sequence	D87086	554	ENA	1091042 tax ID	*
[Ref.: #20218]	P.solanacearum (ATCC 11696) 16S ribosomal RNA	X67036	1470	ENA	1091042 tax ID	*
[Ref.: #20218]	Ralstonia solanacearum strain LMG2299T 16S-23S ribosomal RNA intergenic spacer, partial sequence	KC756967	501	ENA	305 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Ralstonia solanacearum NCPPB 325	GCA_015910705	contig	GenBank	305 tax ID	*
[Ref.: #66792]	Ralstonia solanacearum K60	GCA_002251695	contig	GenBank	1091042 tax ID	*
[Ref.: #66792]	Ralstonia solanacearum K60	GCA_000285815	contig	GenBank	1091042 tax ID	*
[Ref.: #66792]	Ralstonia solanacearum strain NCPPB 325	305.901	wgs	PATRIC	305 tax ID	*
[Ref.: #66792]	Ralstonia solanacearum K60	2537561801	draft	JGI IMG/M	1091042 tax ID	*

[Ref.: #3633]

GC-content

66.6 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #3633]

Culture collection no.

DSM 9544, ATCC 11696, ICMP 5712, ICPB PS 256, LMG 2299, NCPPB 325, CCUG 14272, JCM 10489, LMG 17137, UQRS 426, CIP 104762, NRRL B-3212

[Ref.: #71730]

SI-ID 4245

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Preventing Ralstonia solanacearum adhesion with glycans from cashew, cocoa, coffee, pumpkin, and tomato seed extract.	Rachmaninov O, Zinger-Yosovich KD, Gilboa-Garber N	Can J Microbiol	10.1139/w2012-062	2012	*

References

#3633

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 9544

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#46075 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 14272

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71730

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID4245.1 )

* These data were automatically processed and therefore are not curated

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