Strain identifier

BacDive ID: 204

Type strain: Yes

Species: Arcanobacterium canis

Strain Designation: P6775

Strain history: CIP <- 2011, P. Kämpfer, Giessen Univ., Giessen, Germany: strain P6775

NCBI tax ID(s): 999183 (species)

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General

@ref: 17844

BacDive-ID: 204

DSM-Number: 25104

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, animal pathogen

description: Arcanobacterium canis P6775 is an anaerobe, mesophilic, Gram-positive animal pathogen that was isolated from canine otitis externa.

NCBI tax id

  • NCBI tax id: 999183
  • Matching level: species

strain history

@refhistory
375942011, P. Kämpfer, Inst. Angewandte Mikrobiol., Giessen, Germany: strain P6775
17844<- P. Kämpfer, Univ. Giessen, Germany; P6775 <- E. Prenger-Berninghoff
117134CIP <- 2011, P. Kämpfer, Giessen Univ., Giessen, Germany: strain P6775

doi: 10.13145/bacdive204.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Arcanobacterium
  • species: Arcanobacterium canis
  • full scientific name: Arcanobacterium canis Hijazin et al. 2012

@ref: 17844

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Arcanobacterium

species: Arcanobacterium canis

full scientific name: Arcanobacterium canis Hijazin et al. 2012

strain designation: P6775

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
22974positive1.0-2.0 µm0.5 µmrod-shapedno
117134positiverod-shapedno

colony morphology

  • @ref: 62657
  • incubation period: 2 days

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
37594MEDIUM 45 - for Columbia agar with sheep bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Sheepblood (100.000 ml)
17844PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yesName: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled waterhttps://mediadive.dsmz.de/medium/104
17844COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yesName: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar basehttps://mediadive.dsmz.de/medium/693
117134CIP Medium 45yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45

culture temp

@refgrowthtypetemperaturerange
17844positivegrowth37mesophilic
37594positivegrowth37mesophilic
62657positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
17844anaerobe
62657microaerophile
117134microaerophile

spore formation

  • @ref: 22974
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2297416899D-mannitol-builds acid from
2297465327D-xylose-builds acid from
2297417634D-glucose+builds acid from
2297416988D-ribose+builds acid from
2297428087glycogen+builds acid from
2297417716lactose+builds acid from
2297417306maltose+builds acid from
2297417992sucrose+builds acid from
11713417632nitrate-reduction
11713416301nitrite-reduction
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose+fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
11713435581indoleno
6838035581indoleno

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
22974alkaline phosphatase+3.1.3.1
22974alpha-glucosidase+3.2.1.20
22974alpha-mannosidase+3.2.1.24
22974amylase+
22974beta-galactosidase+3.2.1.23
22974beta-glucuronidase+3.2.1.31
22974caseinase-3.4.21.50
22974catalase-1.11.1.6
22974DNase+
22974gelatinase-
22974hyaluronate lyase-4.2.2.1
22974N-acetyl-beta-glucosaminidase+3.2.1.52
22974nitrate reductase-1.7.99.4
22974pyrazinamidase-3.5.1.B15
22974pyrrolidonyl arylamidase-3.4.19.3
22974urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate+
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
117134oxidase-
117134catalase-1.11.1.6
117134urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117134-+++-+--+-+++--+-+--

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
17844--++++---+++----+-+----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
17844canine otitis externaHessen, GiessenGermanyDEUEurope
62657Canine,otitis externaGiessen/HessenGermanyDEUEurope2011
117134Animal, Canine otidis externaGiessen, HessenGermanyDEUEurope2011

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Canidae (Dog)
#Host Body-Site#Organ#Ear

taxonmaps

  • @ref: 69479
  • File name: preview.99_169223.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_28;96_49;97_52;98_77582;99_169223&stattab=map
  • Last taxonomy: Arcanobacterium canis
  • 16S sequence: FR846134
  • Sequence Identity:
  • Total samples: 601
  • soil counts: 7
  • aquatic counts: 10
  • animal counts: 584

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
17844yes, in single cases1Risk group (German classification)
1171342Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Arcanobacterium canis genomic DNA containing 16S rDNA-23S rDNA IGS, strain CIP 110339, isolate P6775-11HE616814345ena999183
17844Arcanobacterium canis partial 16S rRNA gene, type strain DSM 25104TFR8461341363ena999183

Genome sequences

  • @ref: 66792
  • description: Arcanobacterium canis DSM 25104
  • accession: GCA_029625435
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 999183

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno93.839yes
flagellatedno93.858no
gram-positiveno53.237yes
anaerobicno69.37no
aerobicno89.901yes
halophileno79.481no
spore-formingno96.598no
glucose-fermentyes78.317no
thermophileno96.288yes
glucose-utilyes86.628no

External links

@ref: 17844

culture collection no.: DSM 25104, CCM 7958, CCUG 61573, CIP 110339

straininfo link

  • @ref: 69887
  • straininfo: 381143

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22081713Arcanobacterium canis sp. nov., isolated from otitis externa of a dog, and emended description of the genus Arcanobacterium Collins et al. 1983 emend. Yassin et al. 2011.Hijazin M, Prenger-Berninghoff E, Sammra O, Alber J, Lammler C, Kampfer P, Glaeser SP, Busse HJ, Hassan AA, Abdulmawjood A, Zschock MInt J Syst Evol Microbiol10.1099/ijs.0.037150-02011Animals, Arcanobacterium/*classification/genetics/isolation & purification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Dogs/*microbiology, Fatty Acids/analysis, Molecular Sequence Data, Otitis Externa/*microbiology, *Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny24144861Further characteristics of Arcanobacterium canis, a novel species of genus Arcanobacterium.Sammra O, Balbutskaya A, Zhang S, Hijazin M, Nagib S, Lammler C, Abdulmawjood A, Prenger-Berninghoff E, Kostrzewa M, Timke MVet Microbiol10.1016/j.vetmic.2013.09.0172013Actinomycetales Infections/microbiology/*veterinary, Animals, Arcanobacterium/*classification/*genetics, Cat Diseases/*microbiology, Cats, DNA, Ribosomal Spacer/genetics, Dog Diseases/*microbiology, Dogs, Genotype, Molecular Sequence Data, Otitis Externa/microbiology/veterinary, Phylogeny, RNA, Ribosomal, 16S/genetics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methodsGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17844Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25104)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25104
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22974Muaz Hijazin,Ellen Prenger-Berninghoff,Osama Sammra,Jörg Alber,Christoph Lämmler,Peter Kämpfer,Stefanie P. Glaeser,Hans-Jürgen Busse,Abdulwahed Ahmed Hassan,Amir Abdulmawjood,Michael Zschöck10.1099/ijs.0.037150-0Arcanobacterium canis sp. nov., isolated from otitis externa of a dog, and emended description of the genus Arcanobacterium Collins et al. 1983 emend. Yassin et al. 2011IJSEM 62: 2201-2205 201222081713
37594Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8187
62657Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 61573)https://www.ccug.se/strain?id=61573
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69887Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID381143.1StrainInfo: A central database for resolving microbial strain identifiers
117134Curators of the CIPCollection of Institut Pasteur (CIP 110339)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110339