Strain identifier
BacDive ID: 1992
Type strain: 
Species: Paraburkholderia denitrificans
Strain Designation: KIS30-44
Strain history: <- K. Soon-Wo, KACC; KIS30-44 <- H.-Y. Weon
NCBI tax ID(s): 694025 (species)
version 8.1 (current version)
General
@ref: 17652
BacDive-ID: 1992
DSM-Number: 24336
keywords: 16S sequence, Bacteria, mesophilic
description: Paraburkholderia denitrificans KIS30-44 is a mesophilic bacterium that was isolated from forest soil.
NCBI tax id
- NCBI tax id: 694025
- Matching level: species
strain history
- @ref: 17652
- history: <- K. Soon-Wo, KACC; KIS30-44 <- H.-Y. Weon
doi: 10.13145/bacdive1992.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Burkholderiaceae
- genus: Paraburkholderia
- species: Paraburkholderia denitrificans
- full scientific name: Paraburkholderia denitrificans (Lee et al. 2013) Sawana et al. 2015
synonyms
- @ref: 20215
- synonym: Burkholderia denitrificans
@ref: 17652
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Burkholderiaceae
genus: Paraburkholderia
species: Paraburkholderia denitrificans
full scientific name: Paraburkholderia denitrificans (Lee et al. 2013) Sawana et al. 2015
strain designation: KIS30-44
type strain: yes
Morphology
colony morphology
- @ref: 17652
- type of hemolysis: gamma
Culture and growth conditions
culture medium
- @ref: 17652
- name: R2A MEDIUM (DSMZ Medium 830)
- growth: yes
- link: https://mediadive.dsmz.de/medium/830
- composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
culture temp
- @ref: 17652
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | - | assimilation |
| 68369 | 17128 | adipate | - | assimilation |
| 68369 | 27689 | decanoate | - | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 16899 | D-mannitol | - | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 17634 | D-glucose | - | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | - | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | - | 3.2.1.21 |
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 17652 | + | - | - | - | - | - | - | - | - | - | - | - | +/- | - | + | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 17652
- sample type: forest soil
- geographic location: Dokdo Island
- country: Republic of Korea
- origin.country: KOR
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Forest |
| #Environmental | #Terrestrial | #Soil |
Safety information
risk assessment
- @ref: 17652
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 17652
- description: Burkholderia denitrificans strain KIS30-44 16S ribosomal RNA gene, partial sequence
- accession: GU171384
- length: 1416
- database: ena
- NCBI tax ID: 694025
GC content
- @ref: 17652
- GC-content: 63.5
- method: high performance liquid chromatography (HPLC)
External links
@ref: 17652
culture collection no.: DSM 24336, KACC 12733
straininfo link
- @ref: 71639
- straininfo: 408343
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 23124756 | Burkholderia denitrificans sp. nov., isolated from the soil of Dokdo Island, Korea. | Lee CM, Weon HY, Yoon SH, Kim SJ, Koo BS, Kwon SW | J Microbiol | 10.1007/s12275-012-1554-2 | 2012 | Base Composition, Burkholderia/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, Fatty Acids/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, *Soil Microbiology | Metabolism |
| Phylogeny | 30456756 | Paraburkholderia dokdonella sp. nov., isolated from a plant from the genus Campanula. | Jung MY, Kang MS, Lee KE, Lee EY, Park SJ | J Microbiol | 10.1007/s12275-019-8500-5 | 2018 | Bacterial Typing Techniques, Base Composition, Benzoquinones, Burkholderiaceae/*classification/genetics/*isolation & purification/physiology, Campanulaceae/*microbiology, DNA, Bacterial/analysis, Fatty Acids/analysis, Islands, Nucleic Acid Hybridization, Phosphatidylethanolamines/analysis, Phosphatidylglycerols/analysis, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Soil Microbiology, Species Specificity, Temperature, Ubiquinone | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 17652 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24336) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-24336 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 68369 | Automatically annotated from API 20NE | |||
| 71639 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID408343.1 | StrainInfo: A central database for resolving microbial strain identifiers |