Strain identifier

BacDive ID: 1952

Type strain: Yes

Species: Burkholderia cenocepacia

Strain Designation: CF5610, J2315

Strain history: CIP <- 2004, CCUG <- 2003, LMG <- 1995, J. Govan, Edinburgh Univ., Edinburgh, UK: strain J2315

NCBI tax ID(s): 95486 (species)

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General

@ref: 6505

BacDive-ID: 1952

DSM-Number: 16553

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped, antibiotic resistance

description: Burkholderia cenocepacia CF5610 is an obligate aerobe, mesophilic, Gram-negative bacterium that has multiple antibiotic resistances and was isolated from sputum from cystic fibrosis patient.

NCBI tax id

  • NCBI tax id: 95486
  • Matching level: species

strain history

@refhistory
6505<- LMG; LMG 16656 <- J. Govan; CF5610
117244CIP <- 2004, CCUG <- 2003, LMG <- 1995, J. Govan, Edinburgh Univ., Edinburgh, UK: strain J2315

doi: 10.13145/bacdive1952.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Burkholderia
  • species: Burkholderia cenocepacia
  • full scientific name: Burkholderia cenocepacia Vandamme et al. 2003

@ref: 6505

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Burkholderia

species: Burkholderia cenocepacia

full scientific name: Burkholderia cenocepacia Vandamme et al. 2003

strain designation: CF5610, J2315

type strain: yes

Morphology

cell morphology

  • @ref: 117244
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 6505
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6505COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
37374MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
6505TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
117244CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
6505positivegrowth30mesophilic
37374positivegrowth30mesophilic
117244positivegrowth25-41
117244nogrowth5psychrophilic
117244nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 117244
  • oxygen tolerance: obligate aerobe

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
6505Mueller-Hinton Agar301aerob000000000081000000080000000100001000000
6505Mueller-Hinton Agar00000000000100000000000000010000000000

halophily

@refsaltgrowthtested relationconcentration
117244NaClpositivegrowth0-6 %
117244NaClnogrowth8 %
117244NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836927897tryptophan-energy source
6836917632nitrate-reduction
1172444853esculin-hydrolysis
11724417632nitrate-reduction
11724416301nitrite-reduction
11724417632nitrate-respiration

antibiotic resistance

  • @ref: 117244
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11724435581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
117244oxidase+
117244beta-galactosidase+3.2.1.23
117244alcohol dehydrogenase-1.1.1.1
117244gelatinase-
117244amylase-
117244DNase-
117244caseinase-3.4.21.50
117244catalase+1.11.1.6
117244tween esterase-
117244lecithinase-
117244lipase+
117244lysine decarboxylase-4.1.1.18
117244ornithine decarboxylase-4.1.1.17
117244protease-
117244tryptophan deaminase-
117244urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117244-+++-+----++----+---

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
6505-------++-+++-+++++/-+-
6505-------+++/-+++-+++++++
6505--+/-----+++/-+++-++++++
6505--+++--+++/-+++-+++++++
6505-------+++/-+++-+++/-++++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117244+++-+--+-----+-----+++--+-++++-+++--++-----+++--++++++++++-++++++++----+-+-+++-++++-+-+-+++++++++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateisolation date
6505sputum from cystic fibrosis patientEdinburghUnited KingdomGBREurope55.9533-3.1883
58156Human sputum,cystic fibrosisEdinburghUnited KingdomGBREurope1989
117244Human, Sputum, cystic fibrosisEdinburgh, ScotlandUnited KingdomGBREurope1989

isolation source categories

Cat1Cat2Cat3
#Infection#Disease#Cystic fibrosis
#Infection#Patient
#Host Body Product#Fluids#Sputum

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
65052Risk group (German classification)
1172441Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6505
  • description: Burkholderia cepacia genomovar III 16S ribosomal RNA gene, partial sequence
  • accession: AF148556
  • length: 1408
  • database: ena
  • NCBI tax ID: 95486

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Burkholderia cenocepacia NCTC13227GCA_900446215contigncbi95486
66792Burkholderia cenocepacia strain NCTC1322795486.373wgspatric95486
66792Burkholderia cepacia strain LMG 16656292.25wgspatric292
66792Burkholderia cepacia LMG 166562630968810draftimg292

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes85.837no
flagellatedno81.93no
gram-positiveno97.995no
anaerobicno98.694no
halophileno91.448no
spore-formingno93.745no
thermophileno99.632no
glucose-utilyes86.487no
aerobicyes90.587no
glucose-fermentno88.58no

External links

@ref: 6505

culture collection no.: CIP 108255, DSM 16553, ATCC BAA 245, CCM 4899, CCUG 48434, LMG 16656, NCTC 13227

straininfo link

  • @ref: 71597
  • straininfo: 8887

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12648723Burkholderia cenocepacia sp. nov.--a new twist to an old story.Vandamme P, Holmes B, Coenye T, Goris J, Mahenthiralingam E, LiPuma JJ, Govan JRRes Microbiol10.1016/S0923-2508(03)00026-32003Burkholderia/*classification/genetics, Burkholderia Infections/microbiology, Burkholderia cepacia/genetics, Cystic Fibrosis/microbiology, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Rec A Recombinases/genetics, Sequence Analysis, DNAPathogenicity
Genetics16737826Use of ribosomal promoters from Burkholderia cenocepacia and Burkholderia cepacia for improved expression of transporter protein in Escherichia coli.Yu M, Tsang JSProtein Expr Purif10.1016/j.pep.2006.04.0042006Bacterial Proteins/*genetics, Burkholderia cepacia/*genetics, Carrier Proteins/biosynthesis/*genetics, *Escherichia coli/genetics/growth & development, Gene Expression, Promoter Regions, Genetic/*genetics, Recombinant Proteins/biosynthesis/genetics, Ribosomal Proteins/*genetics
Pathogenicity17219455The complete structure and pro-inflammatory activity of the lipooligosaccharide of the highly epidemic and virulent gram-negative bacterium Burkholderia cenocepacia ET-12 (strain J2315).Silipo A, Molinaro A, Ierano T, De Soyza A, Sturiale L, Garozzo D, Aldridge C, Corris PA, Khan CM, Lanzetta R, Parrilli MChemistry10.1002/chem.2006014062007Burkholderia Infections/epidemiology/microbiology/*pathology, Burkholderia cepacia/genetics/immunology/*pathogenicity, Cystic Fibrosis/epidemiology/microbiology/*pathology, Disease Outbreaks, Humans, Inflammation/*pathology, Lipopolysaccharides/*chemistry/immunology/*pharmacology, Magnetic Resonance Spectroscopy, U937 Cells/drug effects/pathology, Virulence
Metabolism20852798Characterization of liposomes formed by lipopolysaccharides from Burkholderia cenocepacia, Burkholderia multivorans and Agrobacterium tumefaciens: from the molecular structure to the aggregate architecture.D'Errico G, Silipo A, Mangiapia G, Vitiello G, Radulescu A, Molinaro A, Lanzetta R, Paduano LPhys Chem Chem Phys10.1039/c0cp00066c2010Agrobacterium tumefaciens/*metabolism, Burkholderia/*metabolism, Burkholderia cenocepacia/metabolism, Electron Spin Resonance Spectroscopy, Light, Lipopolysaccharides/*chemistry, Liposomes/*chemistry, Neutron Diffraction, Phosphatidylethanolamines/chemistry, Scattering, Radiation, Scattering, Small Angle
Pathogenicity22322958Interaction of environmental Burkholderia cenocepacia strains with cystic fibrosis and non-cystic fibrosis bronchial epithelial cells in vitro.Bevivino A, Pirone L, Pilkington R, Cifani N, Dalmastri C, Callaghan M, Ascenzioni F, McClean SMicrobiology (Reading)10.1099/mic.0.056986-02012Bronchi/cytology, Burkholderia Infections/*pathology, Burkholderia cenocepacia/*pathogenicity, Cell Line, Cystic Fibrosis/*microbiology, Epithelial Cells/*microbiology, Humans, Membrane Proteins/metabolism, Phosphoproteins/metabolism, Rhizosphere, Tight Junctions/microbiology, Zea mays/microbiology, Zonula Occludens-1 ProteinMetabolism
Pathogenicity28587856Optimization and characterization of a murine lung infection model for the evaluation of novel therapeutics against Burkholderia cenocepacia.Vanhoutte B, Cappoen D, Maira BM, Cools F, Torfs E, Coenye T, Martinet W, Caljon G, Maes L, Delputte P, Cos PJ Microbiol Methods10.1016/j.mimet.2017.06.0032017Animals, Anti-Bacterial Agents/administration & dosage/*therapeutic use, Burkholderia Infections/*drug therapy/immunology/microbiology, Burkholderia cenocepacia/*drug effects, Cytokines/biosynthesis, *Disease Models, Animal, Drug Evaluation, Preclinical, Humans, Liver/microbiology, Lung/immunology/*microbiology/pathology, Mice, Mice, Inbred BALB C, Pneumonia/drug therapy/microbiology, Respiratory Tract Infections/*drug therapy/microbiology, Spleen/microbiology, Tobramycin/administration & dosage/*therapeutic use
Phenotype29135920Environmental Burkholderia cenocepacia Strain Enhances Fitness by Serial Passages during Long-Term Chronic Airways Infection in Mice.Bragonzi A, Paroni M, Pirone L, Coladarci I, Ascenzioni F, Bevivino AInt J Mol Sci10.3390/ijms181124172017Adaptation, Physiological, Animals, Bacterial Load, Biofilms, Burkholderia cenocepacia/pathogenicity/*physiology, Chronic Disease, Clone Cells, Colony Count, Microbial, *Environmental Microbiology, *Genetic Fitness, Kaplan-Meier Estimate, Larva/microbiology, Lung/microbiology/pathology, Mice, Mice, Inbred C57BL, Phenotype, Random Amplified Polymorphic DNA Technique, Respiratory Tract Infections/*microbiology, Serial Passage, Virulence

Reference

@idauthorscataloguedoi/urltitle
6505Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16553)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16553
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37374Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5857
58156Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48434)https://www.ccug.se/strain?id=48434
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
71597Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID8887.1StrainInfo: A central database for resolving microbial strain identifiers
117244Curators of the CIPCollection of Institut Pasteur (CIP 108255)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108255