Strain identifier

BacDive ID: 1910

Type strain: No

Species: Burkholderia cepacia

Strain Designation: 8

Strain history: K. Komagata KS 0052 <-- AJ 2404 <-- ATCC 17759 <-- R. Y. Stanier 382.

NCBI tax ID(s): 292 (species)

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General

@ref: 12672

BacDive-ID: 1910

DSM-Number: 50181

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, motile, human pathogen

description: Burkholderia cepacia 8 is a mesophilic, motile human pathogen that was isolated from forest soil.

NCBI tax id

  • NCBI tax id: 292
  • Matching level: species

strain history

@refhistory
12672<- ICPB <- A. C. Hayward; 8 (Pseudomonas multivorans)
67770K. Komagata KS 0052 <-- AJ 2404 <-- ATCC 17759 <-- R. Y. Stanier 382.

doi: 10.13145/bacdive1910.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Burkholderia
  • species: Burkholderia cepacia
  • full scientific name: Burkholderia cepacia (Palleroni and Holmes 1981 ex Burkholder 1950) Yabuuchi et al. 1993

  • synonyms

    • @ref: 20215
    • synonym: Pseudomonas cepacia

@ref: 12672

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Burkholderia

species: Burkholderia cepacia

full scientific name: Burkholderia cepacia (Palleroni and Holmes 1981) Yabuuchi et al. 1993

strain designation: 8

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480yes93.819
6948099.996negative

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12672COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
12672NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
12672positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.994

compound production

  • @ref: 12672
  • compound: polyhydroxyalkanoic acids

observation

  • @ref: 67770
  • observation: quinones: Q-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase+
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
12672-----++++++++-+++++++
12672-----++++++++-+++++++

Isolation, sampling and environmental information

isolation

  • @ref: 12672
  • sample type: forest soil

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_198.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_12;96_18;97_18;98_21;99_198&stattab=map
  • Last taxonomy: Burkholderia
  • 16S sequence: X87275
  • Sequence Identity:
  • Total samples: 5054
  • soil counts: 1055
  • aquatic counts: 564
  • animal counts: 1930
  • plant counts: 1505

Safety information

risk assessment

  • @ref: 12672
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • pathogenicity plant: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Burkholderia cepacia strain ATCC 17759 16S ribosomal RNA gene, partial sequenceAY7413341485ena292
20218B.cepacia 16S rRNA geneX872751535ena292
67770Burkholderia cepacia JCM 2799 gene for 16S ribosomal RNA, partial sequenceLC4200641455ena292

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Burkholderia cepacia NCTC10661GCA_900446185contigncbi292
66792Burkholderia cepacia ATCC 17759GCA_002906895scaffoldncbi292
66792Burkholderia cepacia strain ATCC 17759292.135wgspatric292
66792Burkholderia cepacia strain NCTC10661292.149wgspatric292

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno82.504no
gram-positiveno98.488no
anaerobicno98.664no
aerobicyes91.412no
halophileno92.985no
spore-formingno92.536no
glucose-utilyes89.145yes
thermophileno99.54no
motileyes86.951no
glucose-fermentno88.719yes

External links

@ref: 12672

culture collection no.: DSM 50181, ATCC 17759, ICPB 2745-382, NCIB 9085, JCM 2799, AJ 2404, CCUG 18838, CCUG 2855, CECT 322, KCTC 2504, LMG 1275, LMG 2161, NCIMB 9085, NCTC 10661

straininfo link

  • @ref: 71555
  • straininfo: 3949

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Enzymology1282322The use of detergent-based aqueous two-phase systems for the isolation of extracellular proteins: purification of a lipase from Pseudomonas cepacia.Terstappen GC, Geerts AJ, Kula MRBiotechnol Appl Biochem1992Animals, Burkholderia cepacia/*enzymology, *Detergents, Electrophoresis, Polyacrylamide Gel, *Ethylene Glycols, Lipase/*isolation & purification, SwinePhylogeny
1903038[Comparative studies of the filtration behavior of bacteria and organic particles in porous groundwater conductors. Fundamentals and methods].Matthess G, Bedbur E, Gundermann KO, Loof M, Peters DZentralbl Hyg Umweltmed1991*Bacterial Physiological Phenomena, Enterococcus faecalis/physiology, Escherichia coli/physiology, *Filtration, Fresh Water, Microspheres, Pseudomonas/physiology, Soil, Water Microbiology
Metabolism2173896Regulation of pyrimidine biosynthesis in Pseudomonas cepacia.West TP, Chu CPArch Microbiol1990Aspartate Carbamoyltransferase/*metabolism, DNA Transposable Elements, Mutagenesis, Mutation, Pseudomonas/enzymology/genetics/*metabolism, Pyrimidines/*biosynthesisEnzymology10.1007/BF00276539
Pathogenicity6812315[Isolation of an antibacterial active tropolone from a Pseudomonas cepacia strain].Korth H, Brusewitz G, Pulverer GZentralbl Bakteriol Mikrobiol Hyg A1982Cycloheptanes/*isolation & purification, Enterobacteriaceae/drug effects, Pseudomonas/*analysis, Pseudomonas aeruginosa/drug effects, Staphylococcus/drug effects, Streptococcus/drug effects, Tropolone/*isolation & purification/pharmacologyPhylogeny
Metabolism7576530Pyrimidine synthesis in Burkholderia cepacia ATCC 25416.Li K, West TPLett Appl Microbiol1995Aspartate Carbamoyltransferase/antagonists & inhibitors/metabolism, Burkholderia cepacia/*enzymology/metabolism, Cytidine Deaminase/metabolism, Cytosine Deaminase, Diphosphates/pharmacology, Enzyme Inhibitors/pharmacology, Nucleoside Deaminases/metabolism, Nucleotides/pharmacology, Pyrimidines/*biosynthesis, Uracil/metabolism, Uridine Phosphorylase/metabolismEnzymology10.1111/j.1472-765x.1995.tb01075.x
Metabolism7580051Ornibactin production and transport properties in strains of Burkholderia vietnamiensis and Burkholderia cepacia (formerly Pseudomonas cepacia).Meyer JM, Van VT, Stintzi A, Berge O, Winkelmann GBiometals1995Biological Transport, Burkholderia/classification/genetics/*metabolism, Iron/metabolism, Oligopeptides/*biosynthesis, Ornithine/metabolism, Siderophores/*biosynthesisBiotechnology10.1007/BF00141604
Pathogenicity11273742Invasion of human type II pneumocytes by Burkholderia cepacia.Keig PM, Ingham E, Kerr KGMicrob Pathog2001Adult, Burkholderia cepacia/*pathogenicity/physiology, Cells, Cultured, Humans, Pulmonary Alveoli/*cytology/*microbiology10.1006/mpat.2000.0418
Metabolism19953601Production and characterization of poly-3-hydroxybutyrate from biodiesel-glycerol by Burkholderia cepacia ATCC 17759.Zhu C, Nomura CT, Perrotta JA, Stipanovic AJ, Nakas JPBiotechnol Prog2010*Biofuels, Biomass, Burkholderia cepacia/growth & development/*metabolism, Esterification, Fermentation, Glycerol/*metabolism, Hydroxybutyrates/chemistry/*metabolism, Molecular Weight, Nuclear Magnetic Resonance, Biomolecular, Peptide Chain Termination, Translational, Polyesters/chemistry/*metabolism, Polyhydroxyalkanoates/metabolismBiotechnology10.1002/btpr.355
Metabolism21953365Production of polyhydroxyalkanoates by Burkholderia cepacia ATCC 17759 using a detoxified sugar maple hemicellulosic hydrolysate.Pan W, Perrotta JA, Stipanovic AJ, Nomura CT, Nakas JPJ Ind Microbiol Biotechnol2011Acer/metabolism, Acetates/metabolism, Acetic Acid/metabolism, Benzaldehydes/metabolism, Biodegradation, Environmental, Bioreactors, Burkholderia cepacia/genetics/*metabolism, Cellulose/chemistry/*metabolism, Fermentation, Furaldehyde/metabolism, Levulinic Acids/metabolism, Molecular Weight, Polyhydroxyalkanoates/*biosynthesis, Protein Hydrolysates/chemistry/*metabolism, Wood/metabolism, Xylose/metabolismBiotechnology10.1007/s10295-011-1040-6
Metabolism23037882Estimation of inhibitory effects of hemicellulosic wood hydrolysate inhibitors on PHA production by Burkholderia cepacia ATCC 17759 using response surface methodology.Pan W, Nomura CT, Nakas JPBioresour Technol2012Bioreactors/microbiology, Burkholderia cepacia/*drug effects/*metabolism, Computer Simulation, Models, Biological, Polyhydroxyalkanoates/isolation & purification/*metabolism, Polysaccharides/*chemistry, Protein Hydrolysates/*antagonists & inhibitors/*metabolism, Wood/*chemistryBiotechnology10.1016/j.biortech.2012.08.107
Pathogenicity28756196Inhibition of bacterial biofilms by carboxymethyl chitosan combined with silver, zinc and copper salts.Goncalves RC, da Silva DP, Signini R, Naves PLFInt J Biol Macromol2017Animals, Artemia, Bacteria/*drug effects, Biofilms/*drug effects/growth & development, Chitosan/*analogs & derivatives/chemistry/pharmacology/toxicity, Copper/chemistry, Metals, Heavy/*chemistry, Microbial Sensitivity Tests, Silver/chemistry, Sulfates/chemistry, Zinc/chemistry10.1016/j.ijbiomac.2017.07.048
Genetics29700161Draft Genome Sequence of Burkholderia cepacia ATCC 17759, a Polyhydroxybutyrate-Co-Valerate Copolymer-Producing Bacterium.Crooks C, Palmer JM, Lindner DLGenome Announc201810.1128/genomeA.00348-18

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12672Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 50181)https://www.dsmz.de/collection/catalogue/details/culture/DSM-50181
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71555Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3949.1StrainInfo: A central database for resolving microbial strain identifiers