Strain identifier

BacDive ID: 1898

Type strain: No

Species: Brucella pecoris

Strain Designation: 08RB2781-1

Strain history: <- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany; 08RB2781-1 <- F. Melzer

NCBI tax ID(s): 867683 (species)

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General

@ref: 17416

BacDive-ID: 1898

DSM-Number: 23867

keywords: 16S sequence, Bacteria, mesophilic, animal pathogen

description: Brucella pecoris 08RB2781-1 is a mesophilic animal pathogen that was isolated from domestic boar Sus scrofa domestica.

NCBI tax id

  • NCBI tax id: 867683
  • Matching level: species

strain history

  • @ref: 17416
  • history: <- P. Kämpfer, Justus-Liebig-Universität, Giessen, Germany; 08RB2781-1 <- F. Melzer

doi: 10.13145/bacdive1898.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Brucellaceae
  • genus: Brucella
  • species: Brucella pecoris
  • full scientific name: Brucella pecoris (Kämpfer et al. 2011) Hördt et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Ochrobactrum pecoris

@ref: 17416

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Brucellaceae

genus: Brucella

species: Brucella pecoris

full scientific name: Brucella pecoris (Kämpfer et al. 2011) Hördt et al. 2020

strain designation: 08RB2781-1

type strain: no

Morphology

colony morphology

  • @ref: 17416
  • type of hemolysis: alpha/beta
  • hemolysis ability: 1
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17416TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
17416CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water

culture temp

  • @ref: 17416
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
17416catalase+1.11.1.6
17416cytochrome-c oxidase+1.9.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
17416+---++--+++++++--+--+

Isolation, sampling and environmental information

isolation

  • @ref: 17416
  • sample type: domestic boar Sus scrofa domestica
  • host species: Sus scrofa domestica
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Suidae (Pig,Swine)

Safety information

risk assessment

  • @ref: 17416
  • pathogenicity animal: yes, in single cases
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17416
  • description: Ochrobactrum pecoris partial 16S rRNA gene, strain 08RB2781-1
  • accession: FR668303
  • length: 1386
  • database: ena
  • NCBI tax ID: 867683

External links

@ref: 17416

culture collection no.: DSM 23867, CCM 7823

straininfo link

  • @ref: 71542
  • straininfo: 404725

literature

  • topic: Phylogeny
  • Pubmed-ID: 20952542
  • title: Ochrobactrum pecoris sp. nov., isolated from farm animals.
  • authors: Kampfer P, Huber B, Busse HJ, Scholz HC, Tomaso H, Hotzel H, Melzer F
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.027631-0
  • year: 2010
  • mesh: Animals, Animals, Domestic/microbiology, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Ochrobactrum/*classification/genetics/*isolation & purification, Phospholipids/analysis, Phylogeny, Polyamines/analysis, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Sequence Analysis, DNA, Sheep/*microbiology, Sus scrofa/*microbiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
17416Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23867)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23867
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68369Automatically annotated from API 20NE
71542Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID404725.1StrainInfo: A central database for resolving microbial strain identifiers