Strain identifier
BacDive ID: 18144
Type strain: 
Species: Methanocalculus chunghsingensis
Strain Designation: K1F9705b
Strain history: <- M.-C. Lai, National Chung Hsing Univ., Taiwan; K1F9705b <- M.-C. Lai {1997}
NCBI tax ID(s): 156457 (species)
version 8.1 (current version)
General
@ref: 5288
BacDive-ID: 18144
DSM-Number: 14539
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic, Gram-negative
description: Methanocalculus chunghsingensis K1F9705b is an anaerobe, mesophilic, Gram-negative archaeon that was isolated from marine water of aquaculture fishpond.
NCBI tax id
- NCBI tax id: 156457
- Matching level: species
strain history
- @ref: 5288
- history: <- M.-C. Lai, National Chung Hsing Univ., Taiwan; K1F9705b <- M.-C. Lai {1997}
doi: 10.13145/bacdive18144.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanomicrobiales
- family: Methanocalculaceae
- genus: Methanocalculus
- species: Methanocalculus chunghsingensis
- full scientific name: Methanocalculus chunghsingensis Lai et al. 2004
@ref: 5288
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanomicrobiales
family: Methanocalculaceae
genus: Methanocalculus
species: Methanocalculus chunghsingensis
full scientific name: Methanocalculus chunghsingensis Lai et al. 2004
strain designation: K1F9705b
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.967
Culture and growth conditions
culture medium
- @ref: 5288
- name: METHANOCALCULUS MB MEDIUM (DSMZ Medium 924)
- growth: yes
- link: https://mediadive.dsmz.de/medium/924
- composition: Name: METHANOCALCULUS MB MEDIUM (DSMZ Medium 924) Composition: Na-formate 6.72601 g/l NaHCO3 3.95648 g/l Trypticase peptone 1.97824 g/l Yeast extract 1.97824 g/l Na-acetate 1.58259 g/l MgCl2 x 6 H2O 0.98912 g/l NH4Cl 0.98912 g/l KCl 0.49456 g/l K2HPO4 0.395648 g/l Na2S x 9 H2O 0.24728 g/l L-Cysteine HCl x H2O 0.24728 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l CuSO4 x 5 H2O 9.8912e-05 g/l H3BO3 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water
culture temp
- @ref: 5288
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 5288 | anaerobe | |
| 69480 | anaerobe | 99.396 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.962 |
Isolation, sampling and environmental information
isolation
- @ref: 5288
- sample type: marine water of aquaculture fishpond
- geographic location: Taiwan, near Wong-Kong
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Food production | #Aquaculture |
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Aquatic | #Pond (small) |
| #Host | #Fishes |
Safety information
risk assessment
- @ref: 5288
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 5288
- description: Methanocalculus sp. K1F9705b 16S ribosomal RNA gene, partial sequence
- accession: AF347025
- length: 1254
- database: ena
- NCBI tax ID: 156457
Genome sequences
- @ref: 66792
- description: Methanocalculus chunghsingensis K1F9705b
- accession: GCA_018132105
- assembly level: scaffold
- database: ncbi
- NCBI tax ID: 156457
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | no | 90.784 | no |
| flagellated | no | 97.184 | no |
| gram-positive | no | 97.428 | no |
| anaerobic | yes | 99.451 | yes |
| aerobic | no | 96.077 | yes |
| halophile | no | 79.844 | no |
| spore-forming | no | 94.386 | no |
| thermophile | no | 81.784 | no |
| glucose-util | yes | 73.409 | no |
| glucose-ferment | no | 63.992 | no |
External links
@ref: 5288
culture collection no.: DSM 14539, OCM 772
straininfo link
- @ref: 87130
- straininfo: 130843
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 14742478 | Methanocalculus chunghsingensis sp. nov., isolated from an estuary and a marine fishpond in Taiwan. | Lai MC, Lin CC, Yu PH, Huang YF, Chen SC | Int J Syst Evol Microbiol | 10.1099/ijs.0.02761-0 | 2004 | Base Sequence, DNA Primers, DNA, Ribosomal/genetics, Fisheries, Formates/metabolism, Methanomicrobiales/*classification/genetics/isolation & purification, Microbial Sensitivity Tests, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Taiwan | Genetics |
| Phylogeny | 22578640 | The phylogenetic analysis and putative function of lysine 2,3-aminomutase from methanoarchaea infers the potential biocatalysts for the synthesis of beta-lysine. | Hung CC, Lai MC | J Microbiol Immunol Infect | 10.1016/j.jmii.2011.12.031 | 2012 | Amino Acid Motifs, Cloning, Molecular, Cluster Analysis, Intramolecular Transferases/chemistry/*genetics/*metabolism, Lysine/*analogs & derivatives/metabolism, Methanomicrobiales/*enzymology/genetics, Molecular Sequence Data, *Phylogeny, Protein Conformation, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid | Enzymology |
| 36066251 | Draft Genomes of Methanocalculus taiwanensis P2F9704a(T) and Methanocalculus chunghsingensis K1F9705b(T), Hydrogenotrophic Methanogens Belonging to the Family Methanocalculaceae. | Chen SC, Lai SJ, You YT, Shih CJ, Wu YC, Wu CH, Liao CH | Microbiol Resour Announc | 10.1128/mra.00792-22 | 2022 |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 5288 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14539) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14539 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
| 87130 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID130843.1 | StrainInfo: A central database for resolving microbial strain identifiers |