Name: Methanocalculus chunghsingensis K1F9705b
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 18144

Type strain:

Species: Methanocalculus chunghsingensis

Strain Designation: K1F9705b

Culture col. no.: DSM 14539, OCM 772

Strain history: <- M.-C. Lai, National Chung Hsing Univ., Taiwan; K1F9705b <- M.-C. Lai {1997}

NCBI tax ID(s): 156457 (species)

SI-ID 130843

DSM 14539

Other strains from Methanocalculus chunghsingensis

M. chunghsingensis K1F9705c, DSM 14646
M. chunghsingensis 01F9702c, DSM 14888, OCM 255
M. chunghsingensis 01F9704a, DSM 27849, OCM 747

Section

Methanocalculus chunghsingensis K1F9705b is an anaerobe, mesophilic, Gram-negative archaeon that was isolated from marine water of aquaculture fishpond.

Gram-negative
anaerobe
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanomicrobiales*
	Family	*Methanocalculaceae*
	Genus	*Methanocalculus*
	Species	**Methanocalculus chunghsingensis**
	Full Scientific Name (LPSN)	Methanocalculus chunghsingensis Lai et al. 2004

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.967

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5288]

Culture medium

METHANOCALCULUS MB MEDIUM (DSMZ Medium 924)

[Ref.: #5288]

Culture medium growth

[Ref.: #5288]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5288]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5288]			growth	37 °C

[Ref.: #5288]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5288]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.396

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.962

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5288]	Sample type/isolated from	marine water of aquaculture fishpond
[Ref.: #5288]	Geographic location (country and/or sea, region)	Taiwan, near Wong-Kong
[Ref.: #5288]	Country	China
[Ref.: #5288]	Country ISO 3 Code	CHN
[Ref.: #5288]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Aquaculture
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Pond (small)
#Host	#Fishes	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5288]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5288]	Methanocalculus sp. K1F9705b 16S ribosomal RNA gene, partial sequence	AF347025	1254	ENA	156457 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanocalculus chunghsingensis K1F9705b	GCA_018132105	scaffold	GenBank	156457 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Methanocalculus chunghsingensis K1F9705b NCBI: GCA_018132105.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanocalculus chunghsingensis K1F9705b NCBI: GCA_018132105.1
[Ref.: #69480]	motile	no	90.784	no
[Ref.: #69480]	flagellated	no	97.184	no
[Ref.: #69480]	gram-positive	no	97.428	no
[Ref.: #69480]	anaerobic	yes	99.451	yes
[Ref.: #69480]	aerobic	no	96.077	yes
[Ref.: #69480]	halophile	no	79.844	no
[Ref.: #69480]	spore-forming	no	94.386	no
[Ref.: #69480]	thermophile	no	81.784	no
[Ref.: #69480]	glucose-util	yes	73.409	no
[Ref.: #69480]	glucose-ferment	no	63.992	no

Availability in culture collections External links

[Ref.: #5288]

Culture collection no.

DSM 14539, OCM 772

[Ref.: #87130]

SI-ID 130843

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	The phylogenetic analysis and putative function of lysine 2,3-aminomutase from methanoarchaea infers the potential biocatalysts for the synthesis of beta-lysine.	Hung CC, Lai MC	J Microbiol Immunol Infect	10.1016/j.jmii.2011.12.031	2012	*
Phylogeny	Methanocalculus chunghsingensis sp. nov., isolated from an estuary and a marine fishpond in Taiwan.	Lai MC, Lin CC, Yu PH, Huang YF, Chen SC	Int J Syst Evol Microbiol	10.1099/ijs.0.02761-0	2004	*
	Draft Genomes of Methanocalculus taiwanensis P2F9704a(T) and Methanocalculus chunghsingensis K1F9705b(T), Hydrogenotrophic Methanogens Belonging to the Family Methanocalculaceae.	Chen SC, Lai SJ, You YT, Shih CJ, Wu YC, Wu CH, Liao CH	Microbiol Resour Announc	10.1128/mra.00792-22	2022	*

References

#5288

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14539

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87130

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID130843.1 )

* These data were automatically processed and therefore are not curated

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