Strain identifier

BacDive ID: 17971

Type strain: Yes

Species: Anaerococcus lactolyticus

Strain history: CIP <- 1993, GIFU

NCBI tax ID(s): 525254 (strain), 33032 (species)

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General

@ref: 3161

BacDive-ID: 17971

DSM-Number: 7456

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, Gram-positive, human pathogen

description: Anaerococcus lactolyticus DSM 7456 is an anaerobe, Gram-positive human pathogen that was isolated from vaginal discharge.

NCBI tax id

NCBI tax idMatching level
525254strain
33032species

strain history

@refhistory
3161<- JCM <- T. Ezaki, GIFU 8586
407731993, GIFU
67770GIFU 8586.
120535CIP <- 1993, GIFU

doi: 10.13145/bacdive17971.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Peptoniphilaceae
  • genus: Anaerococcus
  • species: Anaerococcus lactolyticus
  • full scientific name: Anaerococcus lactolyticus (Li et al. 1992) Ezaki et al. 2001

  • synonyms

    • @ref: 20215
    • synonym: Peptostreptococcus lactolyticus

@ref: 3161

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Peptoniphilaceae

genus: Anaerococcus

species: Anaerococcus lactolyticus

full scientific name: Anaerococcus lactolyticus (Li et al. 1992) Ezaki et al. 2001

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3161PYG MEDIUM (MODIFIED) (DSMZ Medium 104)yeshttps://mediadive.dsmz.de/medium/104Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water
40773MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
120535CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperature
3161positivegrowth37
40773positivegrowth37
50542positivegrowth37
67770positivegrowth37

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
3161anaerobe
50542anaerobe

spore formation

  • @ref: 69481
  • spore formation: no
  • confidence: 95

murein

  • @ref: 3161
  • murein short key: A12.02
  • type: A4alpha' L-Lys-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea+hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase+
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase-
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate+
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease+3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
3161+--++-----+------+-+/--++/--+++-+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
3161vaginal discharge
50542Human vaginal dischargeJapanJPNAsia
67770Vaginal discharge
120535Human, Vaginal dischargeJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Urogenital tract#Vagina
#Host Body Product#Urogenital tract#Vaginal secretion

taxonmaps

  • @ref: 69479
  • File name: preview.99_3615.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_172;96_1876;97_2253;98_2766;99_3615&stattab=map
  • Last taxonomy: Anaerococcus lactolyticus subclade
  • 16S sequence: AB673373
  • Sequence Identity:
  • Total samples: 8969
  • soil counts: 37
  • aquatic counts: 78
  • animal counts: 8842
  • plant counts: 12

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
3161yes, in single cases1Risk group (German classification)
1205351Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Anaerococcus lactolyticus strain CCUG 31351 16S ribosomal RNA gene, partial sequenceAF5422331411nuccore33032
20218Anaerococcus lactolyticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 8140AB6733731489nuccore33032
20218Anaerococcus lactolyticus gene for 16S ribosomal RNA, partial sequenceD141541375nuccore33032

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Anaerococcus lactolyticus ATCC 51172525254.4wgspatric525254
66792Anaerococcus lactolyticus ATCC 51172643886145draftimg525254
67770Anaerococcus lactolyticus ATCC 51172GCA_000156575scaffoldncbi525254

GC content

@refGC-contentmethod
316134.0high performance liquid chromatography (HPLC)
6777034thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno95no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes85.191no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)yes83.67yes
69480spore-formingspore-formingAbility to form endo- or exosporesno73.833no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no94.878yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno94.676no
69480flagellatedmotile2+Ability to perform flagellated movementno88.5no

External links

@ref: 3161

culture collection no.: DSM 7456, ATCC 51172, JCM 8140, CCUG 31351, CIP 103725, GIFU 8586

straininfo link

  • @ref: 86958
  • straininfo: 42843

literature

  • topic: Phylogeny
  • Pubmed-ID: 1390111
  • title: Three new species of the genus Peptostreptococcus isolated from humans: Peptostreptococcus vaginalis sp. nov., Peptostreptococcus lacrimalis sp. nov., and Peptostreptococcus lactolyticus sp. nov.
  • authors: Li N, Hashimoto Y, Adnan S, Miura H, Yamamoto H, Ezaki T
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-42-4-602
  • year: 1992
  • mesh: Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, DNA, Bacterial/chemistry, Fermentation, Humans, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Peptostreptococcus/chemistry/*classification/metabolism, Phenotype, Sequence Homology, Nucleic Acid
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3161Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7456)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7456
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40773Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15554
50542Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31351)https://www.ccug.se/strain?id=31351
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
86958Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42843.1StrainInfo: A central database for resolving microbial strain identifiers
120535Curators of the CIPCollection of Institut Pasteur (CIP 103725)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103725