Strain identifier

BacDive ID: 17910

Type strain: Yes

Species: Congregibacter litoralis

Strain Designation: KT71

Strain history: <- R. Amann, Max-Planck-Inst. Marine Microbiology, Bremen, Germany; KT71 <- H. Eilers {1999}

NCBI tax ID(s): 393662 (species)

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General

@ref: 6720

BacDive-ID: 17910

DSM-Number: 17192

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, motile, pleomorphic-shaped

description: Congregibacter litoralis KT71 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from Sea Water.

NCBI tax id

  • NCBI tax id: 393662
  • Matching level: species

strain history

  • @ref: 6720
  • history: <- R. Amann, Max-Planck-Inst. Marine Microbiology, Bremen, Germany; KT71 <- H. Eilers {1999}

doi: 10.13145/bacdive17910.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Cellvibrionales
  • family: Halieaceae
  • genus: Congregibacter
  • species: Congregibacter litoralis
  • full scientific name: Congregibacter litoralis Spring et al. 2009

@ref: 6720

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Halieaceae, not assigned to order

family: Halieaceae

genus: Congregibacter

species: Congregibacter litoralis

full scientific name: Congregibacter litoralis Spring et al. 2009

strain designation: KT71

type strain: yes

Morphology

cell morphology

  • @ref: 43411
  • gram stain: negative
  • cell length: 0.5-4.5 µm
  • cell width: 0.4-0.7 µm
  • cell shape: pleomorphic-shaped
  • motility: yes

colony morphology

@refincubation periodcolony sizecolony colorcolony shapemedium used
67202-3 days
434112-3 days1-2 mmcream coloredcircularmarine agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6720CONGREGIBACTER (SYPHC) MEDIUM (DSMZ Medium 1115)yeshttps://mediadive.dsmz.de/medium/1115Name: CONGREGIBACTER (SYPHC) MEDIUM (DSMZ Medium 1115) Composition: Sea Salt 34.965 g/l HEPES 2.4975 g/l Na-pyruvate 1.0989 g/l Yeast extract 0.999001 g/l NH4Cl 0.0999001 g/l KH2PO4 0.04995 g/l L-Cysteine HCl x H2O 0.03996 g/l L-Histidine 0.03996 g/l MgSO4 x 7 H2O 0.02997 g/l NaCl 0.00999001 g/l MnSO4 x H2O 0.00499501 g/l (NH4)2Ni(SO4)2 x 6 H2O 0.0027972 g/l CoCl2 x 6 H2O 0.0017982 g/l ZnSO4 x 7 H2O 0.0017982 g/l CaCl2 x 2 H2O 0.000999001 g/l FeSO4 x 7 H2O 0.000999001 g/l AlK(SO4)2 x 12 H2O 0.00017982 g/l Pyridoxine hydrochloride 0.00014985 g/l CuSO4 x 5 H2O 9.99001e-05 g/l H3BO3 9.99001e-05 g/l Nicotinic acid 9.99001e-05 g/l Thiamine-HCl x 2 H2O 9.99001e-05 g/l Na2SeO4 9.99001e-05 g/l Na2WO4 x 2 H2O 9.99001e-05 g/l Na2MoO4 x 2 H2O 9.99001e-05 g/l Calcium pantothenate 4.995e-05 g/l Vitamin B12 4.995e-05 g/l p-Aminobenzoic acid 3.996e-05 g/l D-(+)-biotin 9.99001e-06 g/l Distilled water
43411Marine agar (MA)yes

culture temp

@refgrowthtypetemperaturerange
6720positivegrowth28mesophilic
43411positiveoptimum28mesophilic
43411positivegrowth9-33

culture pH

@refabilitytypepHPH range
43411positivegrowth6.5-9alkaliphile
43411positiveoptimum7.5-8

Physiology and metabolism

oxygen tolerance

  • @ref: 43411
  • oxygen tolerance: microaerophile

spore formation

  • @ref: 43411
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43411NaClpositivegrowth1-7 %
43411NaClpositiveoptimum2 %

observation

@refobservation
43411Excretion of transparent slime, which causes an increase of medium viscosity, was frequently observed upon incubation in liquid nutrient-rich complex media under conditions of oxygen saturation.
43411Requirement for the vitamins thiamine, biotin and B-12

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
43411165672-aminobenzoate-assimilation
43411309162-oxoglutarate+growth
43411370543-hydroxybutyrate+carbon source
4341130089acetate+/-growth
4341130089acetate+/-carbon source
4341137080acrylate-assimilation
434112509agar-degradation
4341116449alanine+carbon source
4341158187alginate-degradation
4341133709amino acid+nitrogen source
4341128938ammonium+nitrogen source
4341129016arginine+/-growth
4341135391aspartate+carbon source
4341116150benzoate-assimilation
4341117968butyrate+/-growth
4341127689decanoate-assimilation
4341125646octanoate-assimilation
43411casein-degradation
4341117057cellobiose-assimilation
4341117057cellobiose-carbon source
4341162968cellulose-degradation
4341116947citrate-assimilation
4341116947citrate-carbon source
4341115570D-alanine+growth
4341129990D-aspartate+/-growth
4341115824D-fructose-assimilation
4341112936D-galactose+growth
4341117634D-glucose-assimilation
4341116899D-mannitol-assimilation
4341116313D-proline-assimilation
4341116523D-serine-assimilation
4341141808decane+/-growth
4341117997dinitrogen-nitrogen source
43411370543-hydroxybutyrate+growth
4341124996lactate-assimilation
4341125115malate+growth
4341116991dna-degradation
4341128817dodecane+/-growth
4341118262dodecanoate+growth
4341133403elemental sulfur-anaerobic growth
4341117113erythritol-assimilation
4341116236ethanol-assimilation
4341129034ferric iron-anaerobic growth
4341115740formate-assimilation
4341129806fumarate-aerobic growth
4341128260galactose+carbon source
434115291gelatin-degradation
4341117234glucose-carbon source
4341116856glutathione+growth
4341117754glycerol+carbon source
4341117754glycerol+/-growth
4341115428glycine-assimilation
4341129805glycolate-assimilation
4341132362heptanoate+growth
4341145296hexadecane-assimilation
4341129021hexane-assimilation
4341117120hexanoate+/-growth
4341116977L-alanine+growth
4341130849L-arabinose-assimilation
4341116467L-arginine+growth
4341117196L-asparagine+growth
4341129991L-aspartate+growth
4341117285L-cysteic acid-assimilation
4341117561L-cysteine+/-growth
4341129985L-glutamate+growth
4341129985L-glutamate+carbon source
4341115971L-histidine-assimilation
4341117191L-isoleucine-assimilation
4341118019L-lysine-assimilation
4341116643L-methionine-assimilation
4341115729L-ornithine-assimilation
4341117295L-phenylalanine-assimilation
4341117203L-proline+growth
4341117115L-serine+growth
4341116414L-valine-assimilation
4341128053melibiose-assimilation
4341117790methanol-assimilation
4341117268myo-inositol-assimilation
4341117632nitrate-nitrogen source
4341117632nitrate-anaerobic growth
4341117590octane+/-growth
4341130823oleate+growth
4341116452oxaloacetate+growth
4341126271proline+carbon source
4341128831propanol+/-growth
4341117272propionate+/-growth
4341117272propionate+/-carbon source
4341115361pyruvate+growth
4341115361pyruvate+carbon source
4341127810resorcinol-assimilation
4341117822serine+carbon source
4341128017starch-degradation
4341130031succinate+growth
4341130031succinate+carbon source
4341117992sucrose+growth
4341117992sucrose+carbon source
4341116189sulfate-anaerobic growth
4341115891taurine-nitrogen source
4341115891taurine-assimilation
4341141253tetradecane-assimilation
4341116094thiosulfate-anaerobic growth
4341116199urea+nitrogen source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
43411124991cefalotinyesyes
4341117698chloramphenicolyesyes
4341137943colistinyesyes
4341117833gentamicinyesyes
43411471744imipenemyesyes
434117507neomycinyesyes
434118309polymyxin byesyes
4341128669bacitracinyesyes
4341150845doxycyclineyesyes
434116472lincomycinyesyes
434117809oxacillinyesyes
4341127902tetracyclineyesyes
4341128001vancomycinyesyes

enzymes

@refvalueactivityec
43411urease+3.5.1.5
43411tryptophanase-4.1.99.1
43411arginine dihydrolase-3.5.3.6
43411catalase+/-1.11.1.6
43411cytochrome oxidase+1.9.3.1
43411beta-glucosidase-3.2.1.21

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
6720Sea WaterGerman Bight off HelgolandGermanyDEUEurope
43411water column (8m depth) of the North Sea near HelgolandNorth Sea near HelgolandGermanyDEUEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_7671.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_305;96_1216;97_1442;98_1760;99_7671&stattab=map
  • Last taxonomy: Halieaceae
  • 16S sequence: AY007676
  • Sequence Identity:
  • Total samples: 533
  • soil counts: 5
  • aquatic counts: 491
  • animal counts: 29
  • plant counts: 8

Safety information

risk assessment

  • @ref: 6720
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 6720
  • description: Unknown marine gamma proteobacterium NOR5 16S ribosomal RNA, partial sequence
  • accession: AY007676
  • length: 1389
  • database: ena
  • NCBI tax ID: 314285

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Congregibacter litoralis KT71GCA_000153125scaffoldncbi314285
66792Congregibacter litoralis KT71314285.4wgspatric314285
66792Congregibacter litoralis KT712597489864draftimg314285

GC content

  • @ref: 6720
  • GC-content: 57.8
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes86.492yes
flagellatedno55.327no
gram-positiveno98.454yes
anaerobicno99.074yes
aerobicyes82.457yes
halophileyes74.913no
spore-formingno96.98yes
thermophileno99.095no
glucose-utilyes74.108no
glucose-fermentno90.298no

External links

@ref: 6720

culture collection no.: DSM 17192, CECT 7588, NBRC 104960

straininfo link

  • @ref: 86899
  • straininfo: 363400

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19287491The photosynthetic apparatus and its regulation in the aerobic gammaproteobacterium Congregibacter litoralis gen. nov., sp. nov.Spring S, Lunsdorf H, Fuchs BM, Tindall BJPLoS One10.1371/journal.pone.00048662009*Photosynthesis, Phylogeny, Proteobacteria/classification/growth & development/*physiology/ultrastructure
Phylogeny23705883Taxonomy and evolution of bacteriochlorophyll a-containing members of the OM60/NOR5 clade of marine gammaproteobacteria: description of Luminiphilus syltensis gen. nov., sp. nov., reclassification of Haliea rubra as Pseudohaliea rubra gen. nov., comb. nov., and emendation of Chromatocurvus halotolerans.Spring S, Riedel T, Sproer C, Yan S, Harder J, Fuchs BMBMC Microbiol10.1186/1471-2180-13-1182013Aerobiosis, Aquatic Organisms/chemistry/*classification/*genetics/isolation & purification, Bacteriochlorophyll A/*analysis, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Gammaproteobacteria/chemistry/*classification/*genetics/isolation & purification, Genetic Variation, Germany, Metabolic Networks and Pathways/genetics, Molecular Sequence Data, North Sea, Photosynthesis, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNAEnzymology
Enzymology25006520Function and Evolution of the Sox Multienzyme Complex in the Marine Gammaproteobacterium Congregibacter litoralis.Spring SISRN Microbiol10.1155/2014/5974182014

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6720Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17192)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17192
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
43411Stefan Spring, Heinrich Luensdorf, Bernhard M., Brian J. Tindall10.1371/journal.pone.0004866The Photosynthetic Apparatus and Its Regulation in the Aerobic Gammaproteobacterium Congregibacter litoralis gen. nov., sp. nov.PLoS ONE 4: - 200919287491
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86899Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID363400.1StrainInfo: A central database for resolving microbial strain identifiers