Strain identifier

BacDive ID: 177

Type strain: Yes

Species: Actinomyces bowdenii

Strain Designation: M1327/95/1, M1327/96/1

Strain history: CIP <- 2000, CCUG <- 1997, M.D. Collins , IFR, Reading, UK: strain M1327/96/1 <- G. Foster, SAC, Vet. Div., Inverness, UK

NCBI tax ID(s): 131109 (species)

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General

@ref: 5820

BacDive-ID: 177

DSM-Number: 15435

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped, animal pathogen

description: Actinomyces bowdenii M1327/95/1 is a microaerophile, mesophilic, Gram-positive animal pathogen that was isolated from dog, abscess under the mandible.

NCBI tax id

  • NCBI tax id: 131109
  • Matching level: species

strain history

@refhistory
5820<- CCUG <- M. D. Collins, IFR Reading; M1327/95/1 <- G. Foster, SAC, Inverness
116979CIP <- 2000, CCUG <- 1997, M.D. Collins , IFR, Reading, UK: strain M1327/96/1 <- G. Foster, SAC, Vet. Div., Inverness, UK

doi: 10.13145/bacdive177.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Actinomyces
  • species: Actinomyces bowdenii
  • full scientific name: Actinomyces bowdenii Pascual et al. 1999

@ref: 5820

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Actinomyces

species: Actinomyces bowdenii

full scientific name: Actinomyces bowdenii Pascual et al. 1999

strain designation: M1327/95/1, M1327/96/1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotilityconfidence
23112positive2.0-4.0 µmrod-shapedno
69480no91.454
69480positive100
116979positiverod-shapedno

colony morphology

@reftype of hemolysiscolony sizecolony colorincubation periodmedium used
23112gamma2.0 mmgreyish-white1-2 daysblood agar
116979

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5820BHI/2 MEDIUM (DSMZ Medium 217)yeshttps://mediadive.dsmz.de/medium/217Name: BHI/2 MEDIUM (DSMZ Medium 217) Composition: Brain heart infusion 37.0 g/l Casein hydrolysate 10.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
5820COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
23112blood agaryes
35971MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
116979CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5820positivegrowth37mesophilic
23112growth37mesophilic
35971positivegrowth37mesophilic
116979positivegrowth25-41
116979nogrowth10psychrophilic
116979nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5820microaerophile
23112facultative anaerobe
116979facultative anaerobe

spore formation

@refspore formationconfidence
69481no91
69480no99.984

halophily

@refsaltgrowthtested relationconcentration
116979NaClpositivegrowth0-2 %
116979NaClnogrowth4 %
116979NaClnogrowth6 %
116979NaClnogrowth8 %
116979NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2311223456cyclodextrin-builds acid from
2311218333D-arabitol-builds acid from
2311265327D-xylose-builds acid from
2311230849L-arabinose-builds acid from
2311229864mannitol-builds acid from
2311227941pullulan-builds acid from
2311230911sorbitol-builds acid from
2311233954tagatose-builds acid from
2311217108D-arabinose-fermentation
2311265327D-xylose-fermentation
2311229864mannitol-fermentation
2311237657methyl D-glucoside-fermentation
231125291gelatin-hydrolysis
23112606565hippurate-hydrolysis
2311215688acetoin-other
2311217634D-glucose+builds acid from
2311216634raffinose+builds acid from
2311217716lactose+builds acid from
2311217306maltose+builds acid from
231126731melezitose+builds acid from
2311228053melibiose+builds acid from
23112320055methyl beta-D-glucopyranoside+builds acid from
2311233942ribose+builds acid from
2311217992sucrose+builds acid from
2311227082trehalose+builds acid from
2311217234glucose+fermentation
231126731melezitose+fermentation
2311217992sucrose+fermentation
231124853esculin+hydrolysis
2311217632nitrate+reduction
2311233954tagatose+/-builds acid from
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837116634raffinose+builds acid from
683716731melezitose+builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose+builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol+builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
11697916947citrate-carbon source
1169794853esculin+hydrolysis
116979606565hippurate-hydrolysis
11697917632nitrate+reduction
11697916301nitrite-reduction
11697917632nitrate+respiration

metabolite production

  • @ref: 116979
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11697915688acetoin-
11697917234glucose+

enzymes

@refvalueactivityec
23112alkaline phosphatase+/-3.1.3.1
23112alpha-fucosidase-3.2.1.51
23112alpha-galactosidase+3.2.1.22
23112alpha-glucosidase+/-3.2.1.20
23112alpha-mannosidase-3.2.1.24
23112arginine dihydrolase-3.5.3.6
23112beta-galactosidase+3.2.1.23
23112beta-glucosidase+3.2.1.21
23112beta-glucuronidase-3.2.1.31
23112beta-mannosidase-3.2.1.25
23112chymotrypsin-3.4.4.5
23112cystine arylamidase-3.4.11.3
23112esterase (C 4)-
23112esterase lipase (C 8)-
23112glycyl tryptophan arylamidase-
23112leucine arylamidase+3.4.11.1
23112lipase (C 14)-
23112N-acetyl-beta-glucosaminidase-3.2.1.52
23112pyrazinamidase+3.5.1.B15
23112pyrazinamidase+/-3.5.1.B15
23112pyroglutamic acid arylamidase-
23112trypsin-3.4.21.4
23112urease-3.5.1.5
23112valine arylamidase-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
116979oxidase-
116979beta-galactosidase-3.2.1.23
116979alcohol dehydrogenase-1.1.1.1
116979gelatinase-
116979amylase+
116979DNase-
116979caseinase-3.4.21.50
116979catalase+1.11.1.6
116979tween esterase-
116979gamma-glutamyltransferase-2.3.2.2
116979lecithinase-
116979lipase-
116979lysine decarboxylase-4.1.1.18
116979ornithine decarboxylase-4.1.1.17
116979phenylalanine ammonia-lyase-4.3.1.24
116979protease-
116979tryptophan deaminase-
116979urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116979-----++---++++-++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116979+---+--+/--++++---+---+/---++++/-+++++-+++/-+/--++---------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling date
5820dog, abscess under the mandibleUnited KingdomGBREurope
53660Dog,abscess under the mandibleUnited KingdomGBREurope1996
116979Animal, Dog, abscess under the mandible

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Canidae (Dog)
#Host Body-Site#Other#Abscess

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
5820yes2Risk group (German classification)
1169792Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5820
  • description: Actinomyces bowdenii 16S rRNA gene, strain M1956/95/1
  • accession: AJ234039
  • length: 1513
  • database: ena
  • NCBI tax ID: 131109

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinomyces bowdenii DSM 15435GCA_017592395scaffoldncbi131109
66792Actinomyces bowdenii strain DSM 15435131109.7wgspatric131109

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno91no
flagellatedno97.153yes
gram-positiveyes94.963yes
anaerobicno94.567yes
aerobicno92.508yes
halophileno84.692no
spore-formingno93.41no
thermophileno96.553yes
glucose-utilyes86.663no
motileno90.887yes
glucose-fermentyes70.945no

External links

@ref: 5820

culture collection no.: DSM 15435, CCUG 37421, CIP 106647

straininfo link

  • @ref: 69859
  • straininfo: 13433

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny10555371Actinomyces bowdenii sp. nov., isolated from canine and feline clinical specimens.Pascual C, Foster G, Falsen E, Bergstrom K, Greko C, Collins MDInt J Syst Bacteriol10.1099/00207713-49-4-18731999Actinomyces/*classification/cytology/isolation & purification/physiology, Actinomycosis/microbiology/*veterinary, Animals, Bacterial Typing Techniques, Cat Diseases/*microbiology, Cats, Dog Diseases/*microbiology, Dogs, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny34252022Characterization of isolates of members of the genus Actinomyces from Marmota himalayana: description of Actinomyces faecalis sp. nov., Actinomyces respiraculi sp. nov., and Actinomyces trachealis sp. nov.Zhou J, Zhang S, Zhang G, Yang J, Lai XH, Pu J, Jin D, Lu S, Huang Y, Zhu W, Huang Y, Xu M, Lei W, Cheng Y, Liu L, Xu JInt J Syst Evol Microbiol10.1099/ijsem.0.0048752021Actinomyces/*classification/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Marmota/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, TibetTranscriptome
Phylogeny34536031Actinomyces capricornis sp. nov., isolated from the oral cavity of a Japanese serow.Saito M, Shinozaki-Kuwahara N, Hashizume-Takizawa T, Gotouda H, Senpuku H, Kurita-Ochiai TMicrobiol Immunol10.1111/1348-0421.129412021*Actinomyces/genetics, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids, Japan, Mouth, Nucleic Acid Hybridization, *Phospholipids, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5820Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15435)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15435
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23112Cristina Pascual, Geoffrey Foster, Enevold Falsen, Karin Bergström, Christina Greko, Matthew D. Collins10.1099/00207713-49-4-1873Actinomyces bowdenii sp. nov., isolated from canine and feline clinical specimensIJSEM 49: 1873-1877 199910555371
35971Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18801
53660Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37421)https://www.ccug.se/strain?id=37421
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69859Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13433.1StrainInfo: A central database for resolving microbial strain identifiers
116979Curators of the CIPCollection of Institut Pasteur (CIP 106647)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106647