Archive BacDiveID:10.13145/bacdive17654.20230509.8.1

Strain identifier

BacDive ID: 17654

Type strain:

Species: Natranaerobius thermophilus

Strain Designation: JW/NM-WN-LF

Strain history: <- J. Wiegel; JW/NM-WN-LF

NCBI tax ID(s): 375929 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 7353

BacDive-ID: 17654

DSM-Number: 18059

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, motile

description: Natranaerobius thermophilus JW/NM-WN-LF is an anaerobe, spore-forming, thermophilic bacterium that was isolated from mixed water-sediment sample.

NCBI tax id

NCBI tax id: 375929
Matching level: species

strain history

@ref: 7353
history: <- J. Wiegel; JW/NM-WN-LF

doi: 10.13145/bacdive17654.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/bacillota
domain: Bacteria
phylum: Bacillota
class: Clostridia
order: Natranaerobiales
family: Natranaerobiaceae
genus: Natranaerobius
species: Natranaerobius thermophilus
full scientific name: Natranaerobius thermophilus Mesbah et al. 2007

@ref: 7353

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Natranaerobiales

family: Natranaerobiaceae

genus: Natranaerobius

species: Natranaerobius thermophilus

full scientific name: Natranaerobius thermophilus Mesbah et al. 2007

strain designation: JW/NM-WN-LF

type strain: yes

Morphology

cell morphology

@ref	motility	confidence	gram stain
69480	yes	93.312
69480		100	positive

Culture and growth conditions

culture medium

@ref: 7353
name: NATRANAEROBIUS MEDIUM (DSMZ Medium 1095)
growth: yes
link: https://mediadive.dsmz.de/medium/1095
composition: Name: NATRANAEROBIUS MEDIUM (DSMZ Medium 1095) Composition: NaCl 99.8004 g/l Na2CO3 67.8643 g/l NaHCO3 37.9242 g/l Tryptone 9.98004 g/l Yeast extract 9.98004 g/l Sucrose 4.99002 g/l L-Cysteine HCl x H2O 0.698603 g/l NH4Cl 0.499002 g/l KH2PO4 0.199601 g/l MgCl2 0.0998004 g/l HCl 0.00249501 g/l FeCl2 x 4 H2O 0.00149701 g/l CoCl2 x 6 H2O 0.000189621 g/l MnCl2 x 4 H2O 9.98004e-05 g/l Pyridoxine hydrochloride 9.98004e-05 g/l ZnCl2 6.98603e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Na2MoO4 x 2 H2O 3.59281e-05 g/l NiCl2 x 6 H2O 2.39521e-05 g/l Folic acid 1.99601e-05 g/l Biotin 1.99601e-05 g/l H3BO3 5.98802e-06 g/l CuCl2 x 2 H2O 1.99601e-06 g/l Vitamin B12 9.98004e-07 g/l Distilled water

culture temp

@ref: 7353
growth: positive
type: growth
temperature: 50
range: thermophilic

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance	confidence
7353	anaerobe
69480	anaerobe	100

spore formation

@ref: 69480
spore formation: yes
confidence: 97.03

Isolation, sampling and environmental information

isolation

@ref: 7353
sample type: mixed water-sediment sample
geographic location: Wadi An Natrun Lake Fazda
country: Egypt
origin.country: EGY
continent: Africa

isolation source categories

Cat1: #Environmental
Cat2: #Aquatic
Cat3: #Sediment

taxonmaps

@ref: 69479
File name: preview.99_6399.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_569;96_3031;97_3726;98_4726;99_6399&stattab=map
Last taxonomy: Natranaerobius thermophilus subclade
16S sequence: DQ417202
Sequence Identity:
Total samples: 4915
soil counts: 120
aquatic counts: 204
animal counts: 4558
plant counts: 33

Safety information

risk assessment

@ref: 7353
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 7353
description: Natranaerobius thermophilus JW/NM-WN-LF 16S ribosomal RNA gene, partial sequence
accession: DQ417202
length: 1426
database: ena
NCBI tax ID: 457570

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Natranaerobius thermophilus JW/NM-WN-LF	GCA_000020005	complete	ncbi	457570
66792	Natranaerobius thermophilus JW/NM-WN-LF	457570.21	plasmid	patric	457570
66792	Natranaerobius thermophilus JW/NM-WN-LF	457570.14	complete	patric	457570
66792	Natranaerobius thermophilus JW/NM-WN-LF	457570.20	plasmid	patric	457570
66792	Natranaerobius thermophilus JW/NM-WN-LF	642555142	complete	img	457570

GC content

@ref: 7353
GC-content: 40.4
method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	yes	65	no
motile	yes	81.516	no
flagellated	no	86.603	no
gram-positive	no	67.19	no
anaerobic	yes	98.693	no
aerobic	no	91.81	no
halophile	no	61.879	no
spore-forming	yes	79.042	no
thermophile	yes	95.314	yes
glucose-util	yes	86.647	no
glucose-ferment	no	71.75	no

External links

@ref: 7353

culture collection no.: DSM 18059, ATCC BAA 1301

straininfo link

@ref: 86645
straininfo: 400078

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	17978210	Natranaerobius thermophilus gen. nov., sp. nov., a halophilic, alkalithermophilic bacterium from soda lakes of the Wadi An Natrun, Egypt, and proposal of Natranaerobiaceae fam. nov. and Natranaerobiales ord. nov.	Mesbah NM, Hedrick DB, Peacock AD, Rohde M, Wiegel J	Int J Syst Evol Microbiol	10.1099/ijs.0.65068-0	2007	Bacterial Typing Techniques, Carbonates, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Egypt, Fatty Acids/analysis, Fresh Water/chemistry/microbiology, Genes, rRNA, Genotype, Geologic Sediments/microbiology, Gram-Positive Bacteria/classification/genetics/isolation & purification/physiology, Hot Temperature, Hydrogen-Ion Concentration, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Sodium Chloride/*metabolism	Genetics
Genetics	21642468	Complete genome sequence of the anaerobic, halophilic alkalithermophile Natranaerobius thermophilus JW/NM-WN-LF.	Zhao B, Mesbah NM, Dalin E, Goodwin L, Nolan M, Pitluck S, Chertkov O, Brettin TS, Han J, Larimer FW, Land ML, Hauser L, Kyrpides N, Wiegel J	J Bacteriol	10.1128/JB.05157-11	2011	Alkalies/metabolism, Anaerobiosis, Bacteria/genetics/isolation & purification/metabolism, Base Sequence, Genome, Bacterial, Geologic Sediments/microbiology, Molecular Sequence Data, Sodium Chloride/metabolism	Metabolism

Reference

@id	authors	catalogue	doi/url	title
7353	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18059)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-18059
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
86645	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID400078.1	StrainInfo: A central database for resolving microbial strain identifiers