Strain identifier

BacDive ID: 1744

Type strain: No

Species: Bifidobacterium longum subsp. infantis

Strain Designation: S76E

Strain history: CIP <- 1964, G. Reuter, Inst. Food Hygiene, Free Univ., Berlin-Dahlem, Germany, 'Bifidobacterium liberorum', Bifidobacterium longum: strain S76e

NCBI tax ID(s): 1682 (subspecies)

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General

@ref: 8516

BacDive-ID: 1744

DSM-Number: 20090

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Bifidobacterium longum subsp. infantis S76E is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from intestine of infant.

NCBI tax id

  • NCBI tax id: 1682
  • Matching level: subspecies

strain history

@refhistory
380321964, G. Reuter: strain S76e, Bifidobacterium liberorum
8516<- ATCC (Bifidobacterium infantis) <- G. Reuter, S76E (Bifidobacterium liberorum)
67770DSM 20090 <-- ATCC 15702 <-- G. Reuter S76E ("Bifidobacterium liberorum").
116049CIP <- 1964, G. Reuter, Inst. Food Hygiene, Free Univ., Berlin-Dahlem, Germany, 'Bifidobacterium liberorum', Bifidobacterium longum: strain S76e

doi: 10.13145/bacdive1744.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Bifidobacteriales
  • family: Bifidobacteriaceae
  • genus: Bifidobacterium
  • species: Bifidobacterium longum subsp. infantis
  • full scientific name: Bifidobacterium longum subsp. infantis (Reuter 1963) Mattarelli et al. 2008

  • synonyms

    • @ref: 20215
    • synonym: Bifidobacterium infantis

@ref: 8516

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Bifidobacteriales

family: Bifidobacteriaceae

genus: Bifidobacterium

species: Bifidobacterium longum subsp. infantis

full scientific name: Bifidobacterium longum subsp. infantis (Reuter 1963) Mattarelli et al. 2008

strain designation: S76E

type strain: no

Morphology

cell morphology

  • @ref: 116049
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8516BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)yeshttps://mediadive.dsmz.de/medium/58Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water
38032MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
116049CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
116049CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
8516positivegrowth37mesophilic
38032positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8516anaerobe
116049anaerobe

murein

  • @ref: 8516
  • murein short key: A21.03
  • type: A3ß L-Orn-L-Ser-L-Ala-L-Thr-L-Ala

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371L-arabinose-builds acid from30849
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116049nitrate-reduction17632
116049nitrite-reduction16301

metabolite production

  • @ref: 116049
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
116049oxidase-
116049catalase-1.11.1.6
116049urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116049------------++-++---

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8516---+--+---++++---+-------+--++++---+--------+-----
8516-----+------+------------+--++-+--+---------------
116049-----------------------------+------------------+

Isolation, sampling and environmental information

isolation

@refsample type
8516intestine of infant
67770Intestine of infant
116049Human, Intestine (child)

isolation source categories

Cat1Cat2Cat3
#Host#Human#Child
#Host Body-Site#Gastrointestinal tract

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
85161Risk group (German classification)
1160491Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bifidobacterium longum subsp. infantis gene for 16S rRNA, partial sequence, strain: JCM 1272AB116307475ena1682
20218Bifidobacterium longum gene for 16S rRNA, partial sequence, strain: JCM 1272AB507105664ena216816

GC content

@refGC-contentmethod
851661.4
6777060.3thermal denaturation, midpoint method (Tm)

External links

@ref: 8516

culture collection no.: DSM 20090, ATCC 15702, JCM 1272, BCRC 14602, CECT 4553, CIP 64.71, KCTC 3460, LMG 10500, NCFB 2256, NCDO 2256

straininfo link

  • @ref: 71391
  • straininfo: 264248

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8516Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20090)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20090
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38032Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10177
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
71391Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID264248.1StrainInfo: A central database for resolving microbial strain identifiers
116049Curators of the CIPCollection of Institut Pasteur (CIP 64.71)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2064.71