Strain identifier
BacDive ID: 17408
Type strain: 
Species: Ancylobacter dichloromethanicus
Strain Designation: DM16
Strain history: <- Y. A. Trotsenko, IBFM - Inst. Biochem. Physiol. Microorg., Pushchino, Russia; DM16 <- J.E. Firsova
NCBI tax ID(s): 518825 (species)
version 8.1 (current version)
General
@ref: 15752
BacDive-ID: 17408
DSM-Number: 21507
keywords: 16S sequence, Bacteria, mesophilic
description: Ancylobacter dichloromethanicus DM16 is a mesophilic bacterium that was isolated from dichloromethane contaminated soil.
NCBI tax id
- NCBI tax id: 518825
- Matching level: species
strain history
- @ref: 15752
- history: <- Y. A. Trotsenko, IBFM - Inst. Biochem. Physiol. Microorg., Pushchino, Russia; DM16 <- J.E. Firsova
doi: 10.13145/bacdive17408.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Hyphomicrobiales
- family: Xanthobacteraceae
- genus: Ancylobacter
- species: Ancylobacter dichloromethanicus
- full scientific name: Ancylobacter dichloromethanicus Firsova et al. 2010
@ref: 15752
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Xanthobacteraceae
genus: Ancylobacter
species: Ancylobacter dichloromethanicus
full scientific name: Ancylobacter dichloromethanicus Firsova et al. 2010
strain designation: DM16
type strain: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 15752 | MINERAL MEDIUM WITH VITAMINS (DSMZ Medium 462) | yes | https://mediadive.dsmz.de/medium/462 | Name: MINERAL MEDIUM (BRUNNER) WITH VITAMINS (DSMZ Medium 462; with strain-specific modifications) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l Methanol 5.0 g/l Soy peptone 5.0 g/l NaCl 5.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l Pyridoxamine 0.000125 g/l ZnSO4 x 7 H2O 0.0001 g/l Nicotinic acid 5e-05 g/l Vitamin B12 5e-05 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l Thiamine-HCl x 2 H2O 2.5e-05 g/l p-Aminobenzoate 2.5e-05 g/l NiCl2 x 6 H2O 2e-05 g/l Calcium pantothenate 1.25e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Biotin 5e-06 g/l Distilled water |
| 15752 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
culture temp
- @ref: 15752
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Isolation, sampling and environmental information
isolation
- @ref: 15752
- sample type: dichloromethane contaminated soil
- geographic location: Volgograd
- country: Russia
- origin.country: RUS
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Contamination | |
| #Environmental | #Terrestrial | #Soil |
taxonmaps
- @ref: 69479
- File name: preview.99_6269.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_355;96_2984;97_3669;98_4644;99_6269&stattab=map
- Last taxonomy: Ancylobacter
- 16S sequence: EU589386
- Sequence Identity:
- Total samples: 6101
- soil counts: 1085
- aquatic counts: 2318
- animal counts: 1936
- plant counts: 762
Safety information
risk assessment
- @ref: 15752
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 15752
- description: Ancylobacter dichloromethanicus strain DM16 16S ribosomal RNA gene, partial sequence
- accession: EU589386
- length: 1393
- database: ena
- NCBI tax ID: 518825
GC content
- @ref: 15752
- GC-content: 64.5
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 15752
culture collection no.: DSM 21507, VKM B-2484
straininfo link
- @ref: 86406
- straininfo: 401477
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 19346095 | Ancylobacter dichloromethanicus sp. nov.--a new aerobic facultatively methylotrophic bacterium utilizing dichloromethane. | Firsova J, Doronina N, Lang E, Sproer C, Vuilleumier S, Trotsenko Y | Syst Appl Microbiol | 10.1016/j.syapm.2009.02.002 | 2009 | Alphaproteobacteria/*classification/isolation & purification/metabolism/ultrastructure, Base Sequence, Biodegradation, Environmental, Gram-Negative Aerobic Rods and Cocci/classification/isolation & purification/metabolism/ultrastructure, Methylene Chloride/*metabolism, Molecular Sequence Data, *Soil Microbiology, Soil Pollutants/*metabolism | Metabolism |
| Phylogeny | 28945527 | Ancylobacter pratisalsi sp. nov. with plant growth promotion abilities from the rhizosphere of Plantago winteri Wirtg. | Suarez C, Ratering S, Schafer J, Schnell S | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002320 | 2017 | Alphaproteobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Germany, Phospholipids/chemistry, *Phylogeny, Plantago/growth & development/*microbiology, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Salt-Tolerant Plants/growth & development/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistry | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 15752 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21507) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21507 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 86406 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID401477.1 | StrainInfo: A central database for resolving microbial strain identifiers |