Strain identifier

BacDive ID: 17298

Type strain: Yes

Species: Vibrio hispanicus

Strain Designation: TW 79

Strain history: CIP <- 2004, CAIM <- LMG <- 1993, L. Verdonck, Gent Univ., Belgium: strain TW 79

NCBI tax ID(s): 224312 (species)

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General

@ref: 6514

BacDive-ID: 17298

DSM-Number: 16580

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Vibrio hispanicus TW 79 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from Artemia spp. .

NCBI tax id

  • NCBI tax id: 224312
  • Matching level: species

strain history

@refhistory
6514<- B. Gomez-Gil, CIAD <- LMG <- VIB <- L. Verdonck
119922CIP <- 2004, CAIM <- LMG <- 1993, L. Verdonck, Gent Univ., Belgium: strain TW 79

doi: 10.13145/bacdive17298.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Vibrionales
  • family: Vibrionaceae
  • genus: Vibrio
  • species: Vibrio hispanicus
  • full scientific name: Vibrio hispanicus Gomez-Gil et al. 2004

@ref: 6514

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Vibrionales, not validated

family: Vibrionaceae

genus: Vibrio

species: Vibrio hispanicus

full scientific name: Vibrio hispanicus Gomez-Gil et al. 2004

strain designation: TW 79

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotility
29976negative2.2 µmrod-shapedyes
119922negativerod-shapedyes

pigmentation

  • @ref: 29976
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6514REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b)yeshttps://mediadive.dsmz.de/medium/535bName: REACTIVATION WITH LIQUID MEDIUM (DSMZ Medium 535b) Composition: Trypticase soy broth 30.0 g/l Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l K2HPO4 2.5 g/l D(+)-Glucose 2.5 g/l Distilled water
6514REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a)yeshttps://mediadive.dsmz.de/medium/220aName: REACTIVATION WITH TRYPTONE SOYA BROTH (DSMZ Medium 220a) Composition: Casein peptone 17.0 g/l Agar 15.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
32914MEDIUM 326 - for Cellulophaga balticayesDistilled water make up to (1000.000 ml);Sodium chloride (20.000 g);Tryptic soy agar (40.000 g)
119922CIP Medium 326yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=326

culture temp

@refgrowthtypetemperaturerange
6514positivegrowth28mesophilic
29976positivegrowth04-40
29976positiveoptimum22psychrophilic
32914positivegrowth30mesophilic
61145positivegrowth30mesophilic
119922positivegrowth15-30
119922nogrowth5psychrophilic
119922nogrowth37mesophilic
119922nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
61145aerobe
119922facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
29976NaClpositivegrowth0-10 %
29976NaClpositiveoptimum5 %
119922NaClpositivegrowth0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
29976370543-hydroxybutyrate+carbon source
2997622653asparagine+carbon source
2997635391aspartate+carbon source
2997617057cellobiose+carbon source
2997623652dextrin+carbon source
2997628757fructose+carbon source
2997628260galactose+carbon source
2997624265gluconate+carbon source
299765417glucosamine+carbon source
2997617234glucose+carbon source
2997629987glutamate+carbon source
2997617596inosine+carbon source
2997624996lactate+carbon source
2997617306maltose+carbon source
2997629864mannitol+carbon source
2997637684mannose+carbon source
2997637657methyl D-glucoside+carbon source
2997651850methyl pyruvate+carbon source
2997616634raffinose+carbon source
2997626546rhamnose+carbon source
2997617822serine+carbon source
2997617992sucrose+carbon source
2997617748thymidine+carbon source
2997627082trehalose+carbon source
2997616704uridine+carbon source
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117057cellobiose-builds acid from
683714853esculin+builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837117266L-sorbose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan+energy source
6836917632nitrate+reduction
11992216947citrate-carbon source
1199224853esculin-hydrolysis
11992217234glucose+fermentation
11992217716lactose-fermentation
11992217632nitrate+reduction
11992216301nitrite-reduction
119922132112sodium thiosulfate-builds gas from

antibiotic resistance

  • @ref: 119922
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
2997635581indoleyes
6836935581indoleyes
11992235581indoleyes

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
2997635581indole+
6836935581indole+
11992215688acetoin-
11992217234glucose-

enzymes

@refvalueactivityec
29976alpha-galactosidase+3.2.1.22
29976cytochrome oxidase+1.9.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
119922oxidase+
119922beta-galactosidase+3.2.1.23
119922alcohol dehydrogenase-1.1.1.1
119922gelatinase-
119922amylase+
119922DNase-
119922caseinase+3.4.21.50
119922catalase+1.11.1.6
119922tween esterase-
119922gamma-glutamyltransferase-2.3.2.2
119922lecithinase-
119922lipase-
119922lysine decarboxylase-4.1.1.18
119922ornithine decarboxylase-4.1.1.17
119922phenylalanine ammonia-lyase-4.3.1.24
119922protease+
119922tryptophan deaminase-
119922urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119922-+--------++----+---

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
6514+++--+-++++++++--++-+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119922-----+/----+/-+/-+/-+/--+/---+/----+/---++/--+/---+/-+/----+/-----------+/---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119922--+--------+-+-++-+++--+-------+-----------++----+++--++----+--------------+---++-----+-++++--+----

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
6514Artemia spp. (Brine shrimp)ArtemiaBarcelonaSpainESPEurope
61145Brine shrimp,Artemia spp.SpainESPEurope
119922Environment, WaterBarcelonaSpainESPEurope1991

isolation source categories

  • Cat1: #Host
  • Cat2: #Arthropoda
  • Cat3: #Crustacea

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
65141Risk group (German classification)
1199222Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Vibrio sp. LMG 13240 16S rRNA gene, strain LMG 13240AJ3161781468ena170658
6514Vibrio hispanicus strain LMG 13240 clone 1 16S ribosomal RNA gene, partial sequenceAY2540391516ena224312
6514Vibrio hispanicus strain LMG 13240 clone 2 16S ribosomal RNA gene, partial sequenceAY2540401517ena224312

GC content

  • @ref: 29976
  • GC-content: 42.8

External links

@ref: 6514

culture collection no.: CCUG 56966, CAIM 525, DSM 16580, LMG 13240, VIB 213, CIP 108392

straininfo link

  • @ref: 86298
  • straininfo: 10443

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny14742490Vibrio hispanicus sp. nov., isolated from Artemia sp. and sea water in Spain.Gomez-Gil B, Thompson FL, Thompson CC, Garcia-Gasca A, Roque A, Swings JInt J Syst Evol Microbiol10.1099/ijs.0.02775-02004Animals, Artemia/*microbiology, Gram-Negative Bacteria/classification/isolation & purification, Molecular Sequence Data, Ornithine Decarboxylase/genetics, Phenotype, Phylogeny, Seawater/*microbiology, Vibrio/*classification/genetics/isolation & purificationEnzymology
Phylogeny21596509Vibrio plantisponsor sp. nov., a diazotrophic bacterium isolated from a mangrove associated wild rice (Porteresia coarctata Tateoka).Rameshkumar N, Gomez-Gil B, Sproer C, Lang E, Dinesh Kumar N, Krishnamurthi S, Nair S, Roque ASyst Appl Microbiol10.1016/j.syapm.2011.02.0052011Bacterial Typing Techniques, Base Composition, Base Sequence, DNA Gyrase/genetics, Fatty Acids/chemistry, Genes, Bacterial, Genes, rRNA, Molecular Sequence Data, Multilocus Sequence Typing, Nucleic Acid Hybridization/methods, Oxidoreductases/genetics, Phenotype, Phylogeny, Plant Roots/microbiology, Poaceae/*microbiology, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Sequence Alignment, Vibrio/chemistry/*classification/genetics/*isolation & purificationGenetics
Phylogeny26296768Vibrio oceanisediminis sp. nov., a nitrogen-fixing bacterium isolated from an artificial oil-spill marine sediment.Kang SR, Srinivasan S, Lee SSInt J Syst Evol Microbiol10.1099/ijsem.0.0004562015Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Molecular Sequence Data, *Nitrogen Fixation, Nucleic Acid Hybridization, *Petroleum Pollution, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vibrio/*classification/genetics/isolation & purificationGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
6514Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16580)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16580
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
29976Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126344
32914Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6009
61145Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 56966)https://www.ccug.se/strain?id=56966
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
86298Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID10443.1StrainInfo: A central database for resolving microbial strain identifiers
119922Curators of the CIPCollection of Institut Pasteur (CIP 108392)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108392