Archive BacDiveID:10.13145/bacdive17189.20230509.8.1

Strain identifier

BacDive ID: 17189

Type strain:

Species: Sporomusa ovata

Strain Designation: H1

Strain history: <- R. Oßmer, Universität Göttingen, Institut für Mikrobiologie, Germany; H1 <- B. H. Howard {1981}

NCBI tax ID(s): 1123288 (strain), 2378 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 1194

BacDive-ID: 17189

DSM-Number: 2662

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, motile

description: Sporomusa ovata H1 is an anaerobe, mesophilic, motile bacterium that was isolated from sugar beet leaf silage.

NCBI tax id

NCBI tax id	Matching level
2378	species
1123288	strain

strain history

@ref: 1194
history: <- R. Oßmer, Universität Göttingen, Institut für Mikrobiologie, Germany; H1 <- B. H. Howard {1981}

doi: 10.13145/bacdive17189.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/bacillota
domain: Bacteria
phylum: Bacillota
class: Negativicutes
order: Selenomonadales
family: Sporomusaceae
genus: Sporomusa
species: Sporomusa ovata
full scientific name: Sporomusa ovata Möller et al. 1985

@ref: 1194

domain: Bacteria

phylum: Firmicutes

class: Negativicutes

order: Selenomonadales

family: Sporomusaceae

genus: Sporomusa

species: Sporomusa ovata

full scientific name: Sporomusa ovata Möller et al. 1985

strain designation: H1

type strain: yes

Morphology

cell morphology

@ref	motility	confidence	gram stain
69480	yes	96.708
69480		98.972	negative

Culture and growth conditions

culture medium

@ref: 1194
name: SPOROMUSA MEDIUM (BETAINE) (DSMZ Medium 311)
growth: yes
link: https://mediadive.dsmz.de/medium/311
composition: Name: SPOROMUSA MEDIUM (BETAINE) (DSMZ Medium 311) Composition: Betaine x H2O 6.66667 g/l NaCl 2.23881 g/l Yeast extract 1.99005 g/l Casitone 1.99005 g/l Na2CO3 0.995025 g/l MgSO4 x 7 H2O 0.497512 g/l NH4Cl 0.497512 g/l K2HPO4 0.348259 g/l Na2S x 9 H2O 0.298507 g/l L-Cysteine HCl x H2O 0.298507 g/l CaCl2 x 2 H2O 0.248756 g/l KH2PO4 0.228856 g/l HCl 0.00248756 g/l FeSO4 x 7 H2O 0.00199005 g/l FeCl2 x 4 H2O 0.00149254 g/l NaOH 0.000497512 g/l Sodium resazurin 0.000497512 g/l CoCl2 x 6 H2O 0.000189055 g/l Pyridoxine hydrochloride 9.95025e-05 g/l MnCl2 x 4 H2O 9.95025e-05 g/l ZnCl2 6.96517e-05 g/l p-Aminobenzoic acid 4.97512e-05 g/l Calcium D-(+)-pantothenate 4.97512e-05 g/l Nicotinic acid 4.97512e-05 g/l Riboflavin 4.97512e-05 g/l Thiamine HCl 4.97512e-05 g/l (DL)-alpha-Lipoic acid 4.97512e-05 g/l Na2MoO4 x 2 H2O 3.58209e-05 g/l NiCl2 x 6 H2O 2.38806e-05 g/l Biotin 1.99005e-05 g/l Folic acid 1.99005e-05 g/l H3BO3 5.97015e-06 g/l Na2WO4 x 2 H2O 3.9801e-06 g/l Na2SeO3 x 5 H2O 2.98507e-06 g/l CuCl2 x 2 H2O 1.99005e-06 g/l Vitamin B12 9.95025e-07 g/l Distilled water

culture temp

@ref: 1194
growth: positive
type: growth
temperature: 35
range: mesophilic

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance	confidence
1194	anaerobe
69480	anaerobe	99.999

spore formation

@ref	spore formation	confidence
69481	no	94
69480	yes	99.687

Isolation, sampling and environmental information

isolation

@ref: 1194
sample type: sugar beet leaf silage
geographic location: Göttingen
country: Germany
origin.country: DEU
continent: Europe

isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

Safety information

risk assessment

@ref: 1194
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Sporomusa ovata partial 16S rRNA gene, strain DSM 2662, clone K38	HE962131	1742	ena	2378
20218	Sporomusa ovata partial 16S rRNA gene, strain DSM 2662, clone K35	HE962132	1725	ena	2378
20218	Sporomusa ovata partial 16S rRNA gene, strain DSM 2662, clone K39	HE962133	1719	ena	2378
20218	Sporomusa ovata partial 16S rRNA gene, strain DSM 2662, clone K32	HE962134	1769	ena	2378
20218	Sporomusa ovata partial 16S rRNA gene, strain DSM 2662, clone K34	HE962135	1750	ena	2378
1194	Sporomusa ovata 16S rRNA gene, strain DSM 2662 Type	AJ279800	1500	ena	2378

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Sporomusa ovata DSM 2662	GCA_000445445	contig	ncbi	1123288
66792	Sporomusa ovata DSM 2662	GCA_000423685	scaffold	ncbi	1123288
66792	Sporomusa ovata DSM 2662	1123288.4	wgs	patric	1123288
66792	Sporomusa ovata DSM 2662	1123288.3	wgs	patric	1123288
66792	Sporomusa ovata DSM 2662	2524614594	draft	img	1123288
66792	Sporomusa ovata H1, DSM 2662	2579778983	draft	img	1123288

GC content

@ref: 1194
GC-content: 43.3
method: sequence analysis

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	no	94	no
motile	yes	85.083	no
flagellated	no	55.888	no
gram-positive	no	95.096	no
anaerobic	yes	99.368	yes
aerobic	no	97.533	yes
halophile	no	96.046	no
spore-forming	yes	83.673	no
thermophile	no	96.838	yes
glucose-util	yes	65.233	no
glucose-ferment	no	71.241	no

External links

@ref: 1194

culture collection no.: DSM 2662, ATCC 35899

straininfo link

@ref: 86188
straininfo: 41357

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Metabolism	11133459	Non-growth-associated demethylation of dimethylsulfoniopropionate by (homo)acetogenic bacteria.	Jansen M, Hansen TA	Appl Environ Microbiol	10.1128/AEM.67.1.300-306.2001	2001	Acetyl Coenzyme A/biosynthesis, Betaine/metabolism, Culture Media, Eubacterium/growth & development/metabolism, Genes, rRNA, Gram-Positive Rods/growth & development/metabolism, Methylation, Molecular Sequence Data, Phylogeny, Propionates/metabolism, RNA, Ribosomal, 16S/genetics, Sarcosine/analogs & derivatives/metabolism, Sequence Analysis, DNA, Sulfonium Compounds/*metabolism	Phylogeny
	24029766	First Insights into the Genome of the Gram-Negative, Endospore-Forming Organism Sporomusa ovata Strain H1 DSM 2662.	Poehlein A, Gottschalk G, Daniel R	Genome Announc	10.1128/genomeA.00734-13	2013

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
1194	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2662)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-2662
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481	Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch		10.21203/rs.3.rs-2527258/v1	Predictions based on the model GenomeNet Sporulation v. 1
86188	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID41357.1	StrainInfo: A central database for resolving microbial strain identifiers