Strain identifier
BacDive ID: 17049
Type strain: 
Species: Petrotoga olearia
Strain Designation: SL24
Strain history: S. L'Haridon SL24.
NCBI tax ID(s): 1122955 (strain), 156203 (species)
version 9.1 (current version)
General
@ref: 5090
BacDive-ID: 17049
DSM-Number: 13574
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, Gram-negative
description: Petrotoga olearia SL24 is an anaerobe, Gram-negative bacterium that was isolated from oil/water mixtures from production well-heads of a deep, continental petroleum reservoir.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 156203 | species |
| 1122955 | strain |
strain history
| @ref | history |
|---|---|
| 5090 | <- S. L'Haridon, Centre National de la Recherche Scientifique, and Université de Bretagne Occidentale, Institut Universitaire Européen de la Mer, Plouzané, France; SL24 <- S. L'Haridon {1998} |
| 67770 | S. L'Haridon SL24. |
doi: 10.13145/bacdive17049.20240916.9.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/thermotogota
- domain: Bacteria
- phylum: Thermotogota
- class: Thermotogae
- order: Petrotogales
- family: Petrotogaceae
- genus: Petrotoga
- species: Petrotoga olearia
- full scientific name: Petrotoga olearia L'Haridon et al. 2002
@ref: 5090
domain: Bacteria
phylum: Thermotogae
class: Thermotogae
order: Thermotogales
family: Petrotogaceae
genus: Petrotoga
species: Petrotoga olearia
full scientific name: Petrotoga olearia L'Haridon et al. 2002
strain designation: SL24
type strain: yes
Culture and growth conditions
culture medium
- @ref: 5090
- name: PETROTOGA OLEARIA MEDIUM (DSMZ Medium 959)
- growth: yes
- link: https://mediadive.dsmz.de/medium/959
- composition: Name: PETROTOGA OLEARIA MEDIUM (DSMZ Medium 959) Composition: NaCl 15.0 g/l Maltose 3.5 g/l PIPES 3.4 g/l Na-acetate x 3 H2O 2.72 g/l Trypticase peptone 2.0 g/l Yeast extract 2.0 g/l NH4Cl 1.0 g/l Na2S x 9 H2O 0.5 g/l MgCl2 x 6 H2O 0.5 g/l K2HPO4 0.35 g/l KH2PO4 0.35 g/l KCl 0.2 g/l CaCl2 x 2 H2O 0.1 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
| @ref | growth | type | temperature |
|---|---|---|---|
| 5090 | positive | growth | 55 |
| 67770 | positive | growth | 55 |
Physiology and metabolism
oxygen tolerance
- @ref: 5090
- oxygen tolerance: anaerobe
spore formation
- @ref: 69481
- spore formation: no
- confidence: 99
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 5090 | oil/water mixtures from production well-heads of a deep, continental petroleum reservoir | Western Siberia | Russia | RUS | Asia |
| 67770 | Deep | Western Siberia | Russia | RUS |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Industrial | #Oil reservoir |
| #Environmental | #Aquatic |
Safety information
risk assessment
- @ref: 5090
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 67770
- description: Petrotoga olearia 16S rRNA gene, strain SL24T
- accession: AJ311703
- length: 1475
- database: nuccore
- NCBI tax ID: 156203
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Petrotoga olearia DSM 13574 | 1122955.3 | wgs | patric | 1122955 |
| 66792 | Petrotoga olearia strain DSM 13574 | 156203.3 | wgs | patric | 156203 |
| 66792 | Petrotoga olearia DSM 13574 | 2757320361 | draft | img | 156203 |
| 67770 | Petrotoga olearia DSM 13574 | GCA_002895525 | scaffold | ncbi | 1122955 |
| 67770 | Petrotoga olearia DSM 13574 | GCA_003688505 | scaffold | ncbi | 156203 |
GC content
- @ref: 5090
- GC-content: 35
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| @ref | trait | model | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 69481 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 99 | no |
| 69480 | gram-positive | gram-positive | Positive reaction to Gram-staining | no | 73.145 | no |
| 69480 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | yes | 89.233 | no |
| 69480 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 76.768 | no |
| 69480 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | no | 93.097 | yes |
| 69480 | thermophilic | thermophile | Ability to grow at temperatures above or equal to 45°C | yes | 69.215 | yes |
| 69480 | flagellated | motile2+ | Ability to perform flagellated movement | yes | 54.248 | no |
External links
@ref: 5090
culture collection no.: DSM 13574, JCM 11234
straininfo link
- @ref: 86049
- straininfo: 100861
literature
- topic: Phylogeny
- Pubmed-ID: 12361278
- title: Petrotoga olearia sp. nov. and Petrotoga sibirica sp. nov., two thermophilic bacteria isolated from a continental petroleum reservoir in Western Siberia.
- authors: L'Haridon S, Miroshnichenko ML, Hippe H, Fardeau ML, Bonch-Osmolovskaya EA, Stackebrandt E, Jeanthon C
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/00207713-52-5-1715
- year: 2002
- mesh: Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Gram-Negative Anaerobic Straight, Curved, and Helical Rods/*classification/genetics/*isolation & purification/metabolism, Hot Temperature, Microscopy, Electron, Molecular Sequence Data, Petroleum/microbiology, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Siberia
- topic2: Stress
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 5090 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13574) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13574 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
| 86049 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID100861.1 | StrainInfo: A central database for resolving microbial strain identifiers |