Archive BacDiveID:10.13145/bacdive17016.20230509.8.1

Strain identifier

BacDive ID: 17016

Type strain:

Species: Actinoallomurus radicium

Strain Designation: K08-0182

Strain history: A. Matsumoto K08-0182.

NCBI tax ID(s): 877472 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 17497

BacDive-ID: 17016

DSM-Number: 45523

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic

description: Actinoallomurus radicium K08-0182 is an aerobe, spore-forming, mesophilic bacterium that was isolated from root of a Mondo grass.

NCBI tax id

NCBI tax id: 877472
Matching level: species

strain history

@ref	history
17497	<- Y. Takahashi, Kitasato Universtiy, Japan; K08-0182 <- A. Fukuda
67770	A. Matsumoto K08-0182.

doi: 10.13145/bacdive17016.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/actinomycetota
domain: Bacteria
phylum: Actinomycetota
class: Actinomycetes
order: Streptosporangiales
family: Thermomonosporaceae
genus: Actinoallomurus
species: Actinoallomurus radicium
full scientific name: Actinoallomurus radicium Matsumoto et al. 2012

@ref: 17497

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Thermomonosporaceae

genus: Actinoallomurus

species: Actinoallomurus radicium

full scientific name: Actinoallomurus radicium Matsumoto et al. 2012

strain designation: K08-0182

type strain: yes

Morphology

pigmentation

@ref: 30083
production: yes

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
17497	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	yes	https://mediadive.dsmz.de/medium/65	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
17497	GPHF-MEDIUM (DSMZ Medium 553)	yes	https://mediadive.dsmz.de/medium/553	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
17497	ISP2 MEDIUM (DSMZ Medium 987)	yes	https://mediadive.dsmz.de/medium/987	Name: ISP 2 MEDIUM (DSMZ Medium 987) Composition: Agar 20.0 g/l Malt extract 10.0 g/l Dextrose 4.0 g/l Yeast extract 4.0 g/l Distilled water
17497	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	yes	https://mediadive.dsmz.de/medium/84	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
17497	positive	growth	28	mesophilic
30083	positive	growth	12-36
67770	positive	growth	28	mesophilic

culture pH

@ref: 30083
ability: positive
type: growth
pH: 05-07

Physiology and metabolism

oxygen tolerance

@ref: 30083
oxygen tolerance: aerobe

spore formation

@ref: 30083
spore formation: yes

observation

@ref: 67770
observation: quinones: MK-9(H₆), MK-9(H₈)

metabolite utilization

@ref	Chebi-ID	metabolite	utilization activity	kind of utilization tested
30083	17234	glucose	+	carbon source
30083	18222	xylose	+	carbon source

enzymes

@ref	value	activity	ec
30083	acid phosphatase	+	3.1.3.2
30083	alkaline phosphatase	+	3.1.3.1
30083	gelatinase	+

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent	host species
17497	root of a Mondo grass	Kanagawa Prefecture	Japan	JPN	Asia
67770	Root of a mondo grass (Ophiopogon japonicus)	Kanagawa Pref.	Japan	JPN	Asia	Ophiopogon japonicus

isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)

Safety information

risk assessment

@ref: 17497
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 17497
description: Actinoallomurus radicium gene for 16S rRNA, partial sequence
accession: AB578855
length: 1487
database: ena
NCBI tax ID: 877472

GC content

@ref	GC-content	method
17497	72	high performance liquid chromatography (HPLC)
30083	72-73
67770	72-73	high performance liquid chromatography (HPLC)

External links

@ref: 17497

culture collection no.: DSM 45523, JCM 17294, NBRC 107678

straininfo link

@ref: 86017
straininfo: 397765

literature

topic: Phylogeny
Pubmed-ID: 21421931
title: Actinoallomurus radicium sp. nov., isolated from the roots of two plant species.
authors: Matsumoto A, Fukuda A, Inahashi Y, Omura S, Takahashi Y
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijs.0.029181-0
year: 2011
mesh: Actinomycetales/chemistry/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Diaminopimelic Acid/analysis, Genes, rRNA, Japan, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, Plant Roots/*microbiology, Plants/classification/*microbiology, Poaceae/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Vitamin K 2/analysis
topic2: Genetics

Reference

@id	authors	catalogue	doi/url	title	ID_cross_reference	pubmed
17497	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45523)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-45523
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30083	Barberan A, Caceres Velazquez H, Jones S, Fierer N.		10.1128/mSphere.00237-17	Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information	26440	28776041
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
86017	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID397765.1	StrainInfo: A central database for resolving microbial strain identifiers