Strain identifier

BacDive ID: 1692

Type strain: No

Species: Bifidobacterium bifidum

Strain Designation: 212A

Strain history: ATCC 11863 <-- P. György 212A ("Lactobacillus bifidus subsp. pennsylvanicus").

NCBI tax ID(s): 1681 (species)

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General

@ref: 8650

BacDive-ID: 1692

DSM-Number: 20239

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Bifidobacterium bifidum 212A is an anaerobe, mesophilic bacterium of the family Bifidobacteriaceae.

NCBI tax id

  • NCBI tax id: 1681
  • Matching level: species

strain history

@refhistory
8650<- ATCC <- P. György, 212A (Lactobacillus bifidus subsp. pennsylvanicus)
67770ATCC 11863 <-- P. György 212A ("Lactobacillus bifidus subsp. pennsylvanicus").

doi: 10.13145/bacdive1692.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Bifidobacteriales
  • family: Bifidobacteriaceae
  • genus: Bifidobacterium
  • species: Bifidobacterium bifidum
  • full scientific name: Bifidobacterium bifidum (Tissier 1900) Orla-Jensen 1924 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Bacillus bifidus
    20215Bacterium bifidum

@ref: 8650

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Bifidobacteriales

family: Bifidobacteriaceae

genus: Bifidobacterium

species: Bifidobacterium bifidum

full scientific name: Bifidobacterium bifidum (Tissier 1900) Orla-Jensen 1924 emend. Nouioui et al. 2018

strain designation: 212A

type strain: no

Culture and growth conditions

culture medium

  • @ref: 8650
  • name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/58
  • composition: Name: BIFIDOBACTERIUM MEDIUM (DSMZ Medium 58) Composition: Glucose 10.0 g/l Casein peptone 10.0 g/l Bacto Soytone 5.0 g/l Meat extract 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l MnSO4 x H2O 0.05 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Tween 80 Resazurin Distilled water

culture temp

@refgrowthtypetemperaturerange
8650positivegrowth37mesophilic
46600positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
8650anaerobe
46600anaerobe

murein

  • @ref: 8650
  • murein short key: A21.07
  • type: A4ß L-Orn-D-Ser-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68379nitrate-reduction17632
68379esculin+hydrolysis4853
68379urea-hydrolysis16199
68379gelatin-hydrolysis5291
68379D-ribose-fermentation16988
68379D-xylose-fermentation65327
68379D-mannitol-fermentation16899
68379maltose+fermentation17306
68379lactose+fermentation17716
68379sucrose+fermentation17992
68379glycogen+fermentation28087
68380urea-hydrolysis16199
68380arginine-hydrolysis29016
68380D-mannose+fermentation16024
68380raffinose+fermentation16634
68380L-glutamate-degradation29985
68380nitrate-reduction17632
68380tryptophan-energy source27897
68381arginine-hydrolysis29016
68381D-ribose+builds acid from16988
68381D-mannitol-builds acid from16899
68381sorbitol-builds acid from30911
68381lactose+builds acid from17716
68381trehalose+builds acid from27082
68381raffinose+builds acid from16634
68381sucrose+builds acid from17992
68381L-arabinose-builds acid from30849
68381D-arabitol-builds acid from18333
68381alpha-cyclodextrin-builds acid from40585
68381hippurate-hydrolysis606565
68381glycogen+builds acid from28087
68381pullulan+builds acid from27941
68381maltose+builds acid from17306
68381melibiose+builds acid from28053
68381melezitose-builds acid from6731
68381methyl beta-D-glucopyranoside-builds acid from320055
68381D-tagatose-builds acid from16443
68381urea-hydrolysis16199

metabolite production

@refChebi-IDmetaboliteproduction
6838035581indoleno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6838115688acetoin-
6838035581indole-

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase-3.5.3.6
68380serine arylamidase+
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase+
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
68379catalase-1.11.1.6
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
46600-+---++-+------++++-

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8650----------+++---------------++++-------++---------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
46600--++-++--+++-----++-++-+--+-+

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
46600-++-+-+--++++----++----++++-----

Safety information

risk assessment

  • @ref: 8650
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Bifidobacterium bifidum gene for 16S rRNA, partial sequence, strain: JCM 1209AB116281477ena1681
20218Bifidobacterium bifidum gene for 16S rRNA, partial sequence, strain: JCM 1209AB507076656ena1681
20218Bifidobacterium bifidum strain DSM 20239 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequenceJQ347256743ena1681

External links

@ref: 8650

culture collection no.: DSM 20239, ATCC 11863, JCM 1209, NCDO 795, CCUG 17362, LMG 10645, BCRC 14613, NCDC 795

straininfo link

  • @ref: 71339
  • straininfo: 1240

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny2298702Structure of macroamphiphiles from several Bifidobacterium strains.Iwasaki H, Araki Y, Ito E, Nagaoka M, Yokokura TJ Bacteriol10.1128/jb.172.2.845-852.19901990Bifidobacterium/*analysis, Carbohydrate Conformation, Carbohydrate Sequence, Chromatography, Gel, Chromatography, Ion Exchange, Hydrolysis, Lipopolysaccharides/*isolation & purification, Magnetic Resonance Spectroscopy, Methylation, Molecular Sequence Data, Polysaccharides, Bacterial/isolation & purification, Species SpecificityEnzymology
Enzymology3595606'Lipoteichoic acid' of Bifidobacterium bifidum subspecies pennsylvanicum DSM 20239. A lipoglycan with monoglycerophosphate side chains.Fischer WEur J Biochem10.1111/j.1432-1033.1987.tb11488.x1987Bifidobacterium/*analysis, Chemical Phenomena, Chemistry, Fatty Acids/analysis, Glycerophosphates/*isolation & purification, Hydrogen-Ion Concentration, Hydrolysis, *Lipopolysaccharides, Methylation, Oxidation-Reduction, Phosphates/analysis, Phosphatidic Acids/*isolation & purification, Polysaccharides/analysis, Teichoic Acids/*isolation & purificationPhylogeny
Stress9708252Characterization and antimicrobial spectrum of bifidocin B, a bacteriocin produced by Bifidobacterium bifidum NCFB 1454.Yildirim Z, Johnson MGJ Food Prot10.4315/0362-028x-61.1.471998Bacteria/*drug effects, Bacteriocins/biosynthesis/chemistry/*pharmacology, Bifidobacterium/*chemistry, Endopeptidases, Hot Temperature, Hydrogen-Ion Concentration, Microbial Sensitivity Tests, Molecular Weight, SolventsPathogenicity
Enzymology14762068Purification and characterization of three different types of bile salt hydrolases from Bifidobacterium strains.Kim GB, Yi SH, Lee BHJ Dairy Sci10.3168/jds.S0022-0302(04)73164-12004Amidohydrolases/chemistry/*isolation & purification/metabolism, Amino Acid Sequence, Bifidobacterium/*enzymology, Chromatography, Ion Exchange, Electrophoresis, Polyacrylamide Gel, Isoelectric Point, Substrate SpecificityPhylogeny
Enzymology15345449Cloning and characterization of the bile salt hydrolase genes (bsh) from Bifidobacterium bifidum strains.Kim GB, Miyamoto CM, Meighen EA, Lee BHAppl Environ Microbiol10.1128/AEM.70.9.5603-5612.20042004Amidohydrolases/*genetics, Amino Acid Sequence, Base Sequence, Bifidobacterium/*enzymology/*genetics, Cloning, Molecular, Consensus Sequence, DNA Primers, DNA, Bacterial/genetics, Molecular Sequence Data, RNA, Bacterial/genetics, Sequence Alignment, Sequence Homology, Amino AcidGenetics
Biotechnology18646401Influence of microencapsulation and spray drying on the viability of Lactobacillus and Bifidobacterium strains.Goderska K, Czarnecki ZPol J Microbiol2008Animals, Bifidobacterium/*growth & development, Cryoprotective Agents/chemistry, Desiccation, Drug Compounding, Food Microbiology, Freeze Drying, Lactobacillus acidophilus/*growth & development, *Microbial Viability, Microspheres, Milk/chemistry, *Probiotics, Refrigeration, Starch/chemistry, Temperature
Enzymology22405353Conjugated linoleic and linolenic acid production kinetics by bifidobacteria differ among strains.Gorissen L, De Vuyst L, Raes K, De Smet S, Leroy FInt J Food Microbiol10.1016/j.ijfoodmicro.2012.02.0122012Bifidobacterium/*metabolism, *Fermentation, Isomerism, Kinetics, Linoleic Acids, Conjugated/*biosynthesis, alpha-Linolenic Acid/*biosynthesisBiotechnology
Metabolism22447584Role of extracellular transaldolase from Bifidobacterium bifidum in mucin adhesion and aggregation.Gonzalez-Rodriguez I, Sanchez B, Ruiz L, Turroni F, Ventura M, Ruas-Madiedo P, Gueimonde M, Margolles AAppl Environ Microbiol10.1128/AEM.08024-112012*Bacterial Adhesion, Bifidobacterium/*enzymology/physiology, Cell Membrane/metabolism, Epithelial Cells/*microbiology, HT29 Cells, Humans, Intestines/cytology/*microbiology, Membrane Proteins, Mucins/*chemistry, Probiotics, Transaldolase/*metabolismPathogenicity
Metabolism25542994Comparison of mupirocin-based media for selective enumeration of bifidobacteria in probiotic supplements.Bunesova V, Musilova S, Geigerova M, Pechar R, Rada VJ Microbiol Methods10.1016/j.mimet.2014.12.0162014Anti-Bacterial Agents/*metabolism, Bacterial Load/*methods, Bifidobacterium/drug effects/*growth & development, Colony Count, Microbial/*methods, Culture Media/*chemistry, Mupirocin/*metabolism, Probiotics/*analysisPathogenicity
Metabolism25904284A study of the effect of dietary fiber fractions obtained from artichoke (Cynara cardunculus L. var. scolymus) on the growth of intestinal bacteria associated with health.Fissore EN, Santo Domingo C, Gerschenson LN, Giannuzzi LFood Funct10.1039/c5fo00088b2015Bifidobacterium/*growth & development, Cynara scolymus/chemistry/*metabolism, Dietary Fiber/analysis/*metabolism, Functional Food/analysis, Gastrointestinal Tract/metabolism/*microbiology, Humans, Lactobacillus/*growth & development, Prebiotics/*analysisPathogenicity
Metabolism30840154Assessing inhibitory activity of probiotic culture supernatants against Pseudomonas aeruginosa: a comparative methodology between agar diffusion, broth culture and microcalorimetry.Fredua-Agyeman M, Gaisford SWorld J Microbiol Biotechnol10.1007/s11274-019-2621-12019Agar, Anti-Infective Agents/metabolism/pharmacology, Bacteriological Techniques/*methods, Bifidobacterium, Bifidobacterium animalis/metabolism, Bifidobacterium bifidum/metabolism, Calorimetry/*methods, Culture Media, Hydrogen-Ion Concentration, Lactobacillus acidophilus/metabolism, Microbial Sensitivity Tests/*methods, Probiotics/*metabolism/*pharmacology, Pseudomonas aeruginosa/*drug effects/growth & developmentCultivation

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8650Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20239)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20239
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
46600Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 17362)https://www.ccug.se/strain?id=17362
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68380Automatically annotated from API rID32A
68381Automatically annotated from API rID32STR
71339Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID1240.1StrainInfo: A central database for resolving microbial strain identifiers