Strain identifier

BacDive ID: 168824

Type strain: Yes

Species: Leptospira interrogans

Strain Designation: icterohaemorrhagiae RGA, Leptospira interrogans serogroup Icterohaemorrhagiae, serovar Icterohaemorrhagiae, strain RGA, RGA, LEP1220

Strain history: CIP <- 2002, D. Postic, Inst. Pasteur, Paris, <- -CDC, Atlanta, USA: strain RGA

NCBI tax ID(s): 173 (species)

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For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 20215

BacDive-ID: 168824

DSM-Number: 21525

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Leptospira interrogans icterohaemorrhagiae RGA is a mesophilic bacterium of the family Leptospiraceae.

NCBI tax id

  • NCBI tax id: 173
  • Matching level: species

strain history

  • @ref: 120197
  • history: CIP <- 2002, D. Postic, Inst. Pasteur, Paris, <- -CDC, Atlanta, USA: strain RGA

doi: 10.13145/bacdive168824.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/spirochaetota
  • domain: Bacteria
  • phylum: Spirochaetota
  • class: Spirochaetia
  • order: Leptospirales
  • family: Leptospiraceae
  • genus: Leptospira
  • species: Leptospira interrogans
  • full scientific name: Leptospira interrogans (Stimson 1907) Wenyon 1926 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Spirochaeta interrogans

@ref: 20215

domain: Bacteria

phylum: Spirochaetota

class: Spirochaetia

order: Leptospirales

family: Leptospiraceae

genus: Leptospira

species: Leptospira interrogans

full scientific name: Leptospira interrogans (Stimson 1907) Wenyon 1926 (Approved Lists 1980)

strain designation: icterohaemorrhagiae RGA, Leptospira interrogans serogroup Icterohaemorrhagiae, serovar Icterohaemorrhagiae, strain RGA, RGA, LEP1220

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 120197
  • name: CIP Medium 189
  • growth: yes
  • link: https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=189

culture temp

  • @ref: 120197
  • growth: positive
  • type: growth
  • temperature: 29
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837117754glycerol-builds acid from
6837117113erythritol-builds acid from
6837117108D-arabinose-builds acid from
6837130849L-arabinose-builds acid from
6837116988D-ribose-builds acid from
6837165327D-xylose-builds acid from
6837165328L-xylose-builds acid from
6837115963ribitol-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837112936D-galactose-builds acid from
6837117634D-glucose-builds acid from
6837115824D-fructose-builds acid from
6837116024D-mannose-builds acid from
6837117266L-sorbose-builds acid from
6837162345L-rhamnose-builds acid from
6837116813galactitol-builds acid from
6837117268myo-inositol-builds acid from
6837116899D-mannitol-builds acid from
6837117924D-sorbitol-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837159640N-acetylglucosamine-builds acid from
6837127613amygdalin-builds acid from
6837118305arbutin-builds acid from
683714853esculin-builds acid from
6837117814salicin-builds acid from
6837117057cellobiose-builds acid from
6837117306maltose-builds acid from
6837117716lactose-builds acid from
6837128053melibiose-builds acid from
6837117992sucrose-builds acid from
6837127082trehalose-builds acid from
6837115443inulin-builds acid from
683716731melezitose-builds acid from
6837116634raffinose-builds acid from
6837128017starch-builds acid from
6837128087glycogen-builds acid from
6837117151xylitol-builds acid from
6837128066gentiobiose-builds acid from
6837132528turanose-builds acid from
6837162318D-lyxose-builds acid from
6837116443D-tagatose-builds acid from
6837128847D-fucose-builds acid from
6837118287L-fucose-builds acid from
6837118333D-arabitol-builds acid from
6837118403L-arabitol-builds acid from
6837124265gluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371Potassium 5-ketogluconate-builds acid from

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120197-------------------------------------------------

Isolation, sampling and environmental information

taxonmaps

  • @ref: 69479
  • File name: preview.99_53.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_26;96_43;97_45;98_49;99_53&stattab=map
  • Last taxonomy: Leptospira
  • 16S sequence: Z12817
  • Sequence Identity:
  • Total samples: 572
  • soil counts: 127
  • aquatic counts: 210
  • animal counts: 210
  • plant counts: 25

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
202152Risk group
1201972Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20215
  • description: L.interrogans 16S ribosomal RNA
  • accession: Z12817
  • length: 1396
  • database: ena
  • NCBI tax ID: 173

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Leptospira interrogans serovar Icterohaemorrhagiae str. RGAGCA_000343165contigncbi1291351
66792Leptospira interrogans ATCC 43642GCA_900156205scaffoldncbi173
66792Leptospira interrogans strain ATCC 43642173.142wgspatric173
66792Leptospira interrogans serovar Icterohaemorrhagiae str. RGA1291351.3wgspatric1291351

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes84.697no
flagellatedno81.606no
gram-positiveno98.584no
anaerobicno92.669no
aerobicyes68.804no
halophileno93.608no
spore-formingno88.182no
thermophileno90.33no
glucose-utilyes67.901no
glucose-fermentno88.001no

External links

@ref: 20215

culture collection no.: DSM 21525, ATCC 43642, CRBIP6.1220

straininfo link

  • @ref: 115277
  • straininfo: 38105

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Stress1885754Effect of heat or formalin treatment of leptospires on antibody response detected by immunoblotting.Pope V, Johnson RCJ Clin Microbiol10.1128/jcm.29.7.1548-1550.19911991Animals, Antibodies, Bacterial/*biosynthesis, Antigens, Bacterial/chemistry, Cross Reactions, Formaldehyde, Hot Temperature, Immunoblotting, Leptospira interrogans/classification/*immunology, Molecular Weight, Rabbits, SerotypingPhylogeny
Phylogeny1930573A comparative investigation and identification of Leptospira interrogans serogroup icterohaemorrhagiae strains by monoclonal antibody and DNA fingerprint analyses.Hookey JV, Palmer MFZentralbl Bakteriol10.1016/s0934-8840(11)80065-41991Animals, *Antibodies, Monoclonal, *DNA Fingerprinting, DNA, Bacterial, Leptospira interrogans/*classification/genetics/immunology, Rats
Phylogeny1982104The detection of genetic variation in Leptospira interrogans serogroup ICTEROHAEMORRHAGIAE by ribosomal RNA gene restriction fragment patterns.Hookey JVFEMS Microbiol Lett10.1016/0378-1097(90)90326-l1990DNA Probes, Genes, Genetic Markers, *Genetic Variation, Leptospira interrogans/*genetics, Phylogeny, *Polymorphism, Restriction Fragment Length, RNA, Ribosomal/*chemistry, Restriction Mapping
Enzymology6092434Restriction endonuclease DNA analysis of Leptospira interrogans serovars icterohaemorrhagiae and hebdomadis.Marshall RB, Winter PJ, Yanagawa RJ Clin Microbiol10.1128/jcm.20.4.808-810.19841984DNA Restriction Enzymes/*pharmacology, DNA, Bacterial/*analysis, Electrophoresis, Polyacrylamide Gel, Leptospira interrogans/*analysis/immunology, Molecular Weight, Mutation
Phylogeny6738384Characterization of monoclonal antibodies against etiological agents of Weil's disease.Kobayashi Y, Tamai T, Oyama T, Hasegawa H, Sada E, Kusaba T, Hamaji MMicrobiol Immunol10.1111/j.1348-0421.1984.tb00687.x1984Agglutination, Animals, Antibodies, Monoclonal/*immunology, Hybridomas/immunology, Leptospira interrogans/classification/*immunology, Mice, Species Specificity, Weil Disease/*microbiology
Phylogeny7691982Characterization of Leptospiraceae by 16S DNA restriction fragment length polymorphisms.Hookey JVJ Gen Microbiol10.1099/00221287-139-8-16811993Base Sequence, DNA, Bacterial/genetics, DNA, Ribosomal, Deoxyribonuclease BamHI/metabolism, Deoxyribonuclease EcoRI/metabolism, Leptospiraceae/*classification/genetics, Molecular Sequence Data, *Polymorphism, Restriction Fragment Length, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/*geneticsMetabolism
Phylogeny15545494Proposal to list ATCC 43642 as the type strain of Leptospira interrogans in the Approved Lists of Bacterial Names. Request for an opinion.Landon S, Tang JInt J Syst Evol Microbiol10.1099/ijs.0.63106-02004Leptospira interrogans serovar icterohaemorrhagiae/*classification, *Terminology as Topic
Phylogeny25288661ATCC 43642 replaces ATCC 23581 as the type strain of Leptospira interrogans (Stimson 1907) Wenyon 1926. Opinion 91. Judicial Commission of the International Committee on Systematics of Prokaryotes.Tindall BJInt J Syst Evol Microbiol10.1099/ijs.0.069179-02014Leptospira interrogans/*classification, *Phylogeny, Terminology as Topic

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
115277Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID38105.1
120197Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CRBIP6.1220Collection of Institut Pasteur (CRBIP6.1220)