Archive BacDiveID:10.13145/bacdive16878.20230509.8.1

Strain identifier

BacDive ID: 16878

Type strain:

Species: Thermococcus zilligii

Strain Designation: AN1

Strain history: DSM 2770 <-- H. Morgan AN1.

NCBI tax ID(s): 54076 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 1216

BacDive-ID: 16878

DSM-Number: 2770

keywords: genome sequence, 16S sequence, Archaea, anaerobe, thermophilic

description: Thermococcus zilligii AN1 is an anaerobe, thermophilic archaeon that was isolated from anaerobic mud from alkaline hot spring.

NCBI tax id

NCBI tax id: 54076
Matching level: species

strain history

@ref	history
1216	<- H. Morgan, AN1
67770	DSM 2770 <-- H. Morgan AN1.

doi: 10.13145/bacdive16878.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/archaea
keyword: phylum/methanobacteriota
domain: Archaea
phylum: Methanobacteriota
class: Thermococci
order: Thermococcales
family: Thermococcaceae
genus: Thermococcus
species: Thermococcus zilligii
full scientific name: Thermococcus zilligii Ronimus et al. 1999

@ref: 1216

domain: Archaea

phylum: Euryarchaeota

class: Thermococci

order: Thermococcales

family: Thermococcaceae

genus: Thermococcus

species: Thermococcus zilligii

full scientific name: Thermococcus zilligii Ronimus et al. 1999

strain designation: AN1

type strain: yes

Culture and growth conditions

culture medium

@ref: 1216
name: AN1 MEDIUM (DSMZ Medium 376)
growth: yes
link: https://mediadive.dsmz.de/medium/376
composition: Name: AN1 MEDIUM (DSMZ Medium 376) Composition: Trypticase 10.0 g/l Sulfur 8.0 g/l NaCl 2.5 g/l K2HPO4 1.5 g/l Na-thioglycolate 1.0 g/l Resazurin 0.001 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
1216	positive	growth	75	thermophilic
67770	positive	growth	75	thermophilic

Physiology and metabolism

oxygen tolerance

@ref: 1216
oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
1216	anaerobic mud from alkaline hot spring	Rotorua, Kuirau Park	New Zealand	NZL	Australia and Oceania
67770	Thermal pool at Kuirau Park	Rotorua	New Zealand	NZL	Australia and Oceania

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)
#Condition	#Alkaline
#Condition	#Anoxic (anaerobic)
#Condition	#Thermophilic (>45°C)

taxonmaps

@ref: 69479
File name: preview.99_187098.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17827;96_68785;97_89427;98_121697;99_187098&stattab=map
Last taxonomy: Thermococcus
16S sequence: U76534
Sequence Identity:
Total samples: 41
aquatic counts: 41

Safety information

risk assessment

@ref: 1216
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 1216
description: Thermococcus sp. AN1 16S ribosomal RNA gene, partial sequence
accession: U76534
length: 1451
database: ena
NCBI tax ID: 1151117

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Thermococcus zilligii AN1	2547132157	draft	img	1151117
67770	Thermococcus zilligii AN1	GCA_000258515	contig	ncbi	1151117
66792	Thermococcus zilligii AN1	1151117.4	wgs	patric	1151117

GC content

@ref	GC-content	method
67770	54.48	genome sequence analysis
67770	46.2	thermal denaturation, midpoint method (Tm)

External links

@ref: 1216

culture collection no.: DSM 2770, JCM 10554, ATCC 700529

straininfo link

@ref: 85886
straininfo: 43983

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	9382704	The phylogenetic position of the Thermococcus isolate AN1 based on 16S rRNA gene sequence analysis: a proposal that AN1 represents a new species, Thermococcus zilligii sp. nov.	Ronimus RS, Reysenbach A, Musgrave DR, Morgan HW	Arch Microbiol	10.1007/s002030050495	1997	Archaea/classification/genetics, Bacterial Typing Techniques, Cloning, Molecular, DNA, Ribosomal/genetics, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, Sequence Alignment, Sequence Analysis, DNA	Transcriptome
Enzymology	10591017	The first description of an archaeal hemicellulase: the xylanase from Thermococcus zilligii strain AN1.	Uhl AM, Daniel RM	Extremophiles	10.1007/s007920050126	1999	Amino Acid Sequence, Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Glycoside Hydrolases/chemistry/isolation & purification/metabolism, Hot Temperature, Mass Spectrometry, Molecular Sequence Data, Molecular Weight, Peptide Fragments/chemistry, Substrate Specificity, Thermococcus/*enzymology/growth & development, Xylans/isolation & purification/metabolism	Phylogeny

Reference

@id	authors	catalogue	doi/url	title
1216	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2770)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-2770
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
85886	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID43983.1	StrainInfo: A central database for resolving microbial strain identifiers