Strain identifier

BacDive ID: 16833

Type strain: Yes

Species: Thermoanaerobacter brockii subsp. finnii

Strain Designation: AKo-1

Strain history: <- S. M. Schoberth and H. Giesel, Kernforschungsanlage Jülich, Institut für Biotechnologie, Jülich, Germany; AKo-1 <- S. M. Schoberth {1982}

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General

@ref: 1373

BacDive-ID: 16833

DSM-Number: 3389

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic, alcohol production

description: Thermoanaerobacter brockii subsp. finnii AKo-1 is an anaerobe, thermophilic bacterium that produces alcohol and was isolated from lake sediment.

NCBI tax id

NCBI tax idMatching level
509193strain
29325subspecies

strain history

  • @ref: 1373
  • history: <- S. M. Schoberth and H. Giesel, Kernforschungsanlage Jülich, Institut für Biotechnologie, Jülich, Germany; AKo-1 <- S. M. Schoberth {1982}

doi: 10.13145/bacdive16833.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Thermoanaerobacterales
  • family: Thermoanaerobacteraceae
  • genus: Thermoanaerobacter
  • species: Thermoanaerobacter brockii subsp. finnii
  • full scientific name: Thermoanaerobacter brockii subsp. finnii (Schmid et al. 1986) Cayol et al. 1995
  • synonyms

    • @ref: 20215
    • synonym: Thermoanaerobacter finnii

@ref: 1373

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Thermoanaerobacterales

family: Thermoanaerobacteraceae

genus: Thermoanaerobacter

species: Thermoanaerobacter brockii subsp. finnii

full scientific name: Thermoanaerobacter brockii subsp. finnii (Schmid et al. 1986) Cayol et al. 1995

strain designation: AKo-1

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 1373
  • name: THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/144
  • composition: Name: THERMOANAEROBACTER BROCKII MEDIUM (DSMZ Medium 144) Composition: Trypticase peptone 10.0 g/l D-Glucose 5.0 g/l Yeast extract 3.0 g/l K2HPO4 1.5 g/l Na2S x 9 H2O 1.0 g/l NH4Cl 0.9 g/l KH2PO4 0.75 g/l MgCl2 x 6 H2O 0.4 g/l Nitrilotriacetic acid 0.1152 g/l NaCl 0.009 g/l FeSO4 x 7 H2O 0.003 g/l FeCl2 x 4 H2O 0.0018 g/l CoCl2 x 6 H2O 0.00153 g/l MnCl2 x 4 H2O 0.0009 g/l CaCl2 x 2 H2O 0.0009 g/l ZnCl2 0.0009 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.00027 g/l Na2SeO3 x 5 H2O 0.00027 g/l CuCl2 0.00018 g/l H3BO3 9e-05 g/l Na2MoO4 x 2 H2O 9e-05 g/l Pyridoxine hydrochloride 5e-05 g/l p-Aminobenzoic acid 2.5e-05 g/l (DL)-alpha-Lipoic acid 2.5e-05 g/l Riboflavin 2.5e-05 g/l Calcium D-(+)-pantothenate 2.5e-05 g/l Thiamine HCl 2.5e-05 g/l Nicotinic acid 2.5e-05 g/l Biotin 1e-05 g/l Folic acid 1e-05 g/l Vitamin B12 5e-07 g/l Distilled water

culture temp

  • @ref: 1373
  • growth: positive
  • type: growth
  • temperature: 65
  • range: thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1373
  • oxygen tolerance: anaerobe

compound production

@refcompound
1373amylase
1373ethanol
1373pullulanase

Isolation, sampling and environmental information

isolation

  • @ref: 1373
  • sample type: lake sediment
  • geographic location: Lake Kivu, country of origin unknown

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Sediment

Safety information

risk assessment

  • @ref: 1373
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Thermoanaerobacter finii (DSM 3389) 16S ribosomal RNA (16S rRNA) gene
  • accession: L09166
  • length: 1523
  • database: ena
  • NCBI tax ID: 29325

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Thermoanaerobacter brockii subsp. finnii Ako-1GCA_000175295completencbi509193
66792Thermoanaerobacter brockii subsp. finnii Ako-1509193.4completepatric509193
66792Thermoanaerobacter brockii finnii Ako-1, DSM 3389649633102completeimg509193

GC content

  • @ref: 1373
  • GC-content: 32.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes86.337no
flagellatedno89.136no
gram-positiveno61.08no
anaerobicyes99.23yes
aerobicno98.297yes
halophileno92.913no
spore-formingyes92.958no
glucose-utilyes94.165no
thermophileyes100yes
glucose-fermentyes59.541no

External links

@ref: 1373

culture collection no.: DSM 3389

straininfo link

  • @ref: 85844
  • straininfo: 41924

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1373Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3389)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3389
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
85844Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID41924.1StrainInfo: A central database for resolving microbial strain identifiers