Archive BacDiveID:10.13145/bacdive1676.20230509.8.1

Strain identifier

BacDive ID: 1676

Type strain:

Species: Methyloferula stellata

Strain Designation: AR4

Strain history: <- S. N. Dedysh, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; AR4

NCBI tax ID(s): 876269 (strain), 876270 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 16139

BacDive-ID: 1676

DSM-Number: 22108

keywords: genome sequence, 16S sequence, Bacteria, psychrophilic

description: Methyloferula stellata AR4 is a psychrophilic bacterium that was isolated from acidic peat soil of an oligo-mesotrophic fen.

NCBI tax id

NCBI tax id	Matching level
876270	species
876269	strain

strain history

@ref: 16139
history: <- S. N. Dedysh, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; AR4

doi: 10.13145/bacdive1676.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Alphaproteobacteria
order: Hyphomicrobiales
family: Beijerinckiaceae
genus: Methyloferula
species: Methyloferula stellata
full scientific name: Methyloferula stellata Vorobev et al. 2011

@ref: 16139

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Beijerinckiaceae

genus: Methyloferula

species: Methyloferula stellata

full scientific name: Methyloferula stellata Vorobev et al. 2011

strain designation: AR4

type strain: yes

Culture and growth conditions

culture medium

@ref: 16139
name: METHYLOFERULA STELLATA (DSMZ Medium 1322)
growth: yes
link: https://mediadive.dsmz.de/medium/1322
composition: Name: METHYLOFERULA STELLATA MEDIUM (DSMZ Medium 1322) Composition: Methanol 7.92 g/l KH2PO4 0.1 g/l (NH4)2SO4 0.1 g/l MgSO4 x 7 H2O 0.05 g/l NaCl 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Na2-EDTA 0.00618 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l CuCl2 x 5 H2O 0.0001 g/l Na2MoO4 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l Distilled water

culture temp

@ref: 16139
growth: positive
type: growth
temperature: 23
range: psychrophilic

Isolation, sampling and environmental information

isolation

@ref: 16139
sample type: acidic peat soil of an oligo-mesotrophic fen
geographic location: Archangelsk Region, Torfjanoye (65° 01' N 35° 44' E)
country: Russia
origin.country: RUS
continent: Europe
latitude: 65.0167
longitude: 35.7333

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Wetland (Swamp)
#Environmental	#Terrestrial	#Soil
#Condition	#Acidic

taxonmaps

@ref: 69479
File name: preview.99_2407.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_1297;97_1542;98_1877;99_2407&stattab=map
Last taxonomy: Methyloferula stellata subclade
16S sequence: FR686343
Sequence Identity:
Total samples: 668
soil counts: 326
aquatic counts: 186
animal counts: 96
plant counts: 60

Safety information

risk assessment

@ref: 16139
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 16139
description: Methyloferula stellata AR4 partial 16S rRNA gene, type strain AR4
accession: FR686343
length: 1408
database: ena
NCBI tax ID: 876269

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Methyloferula stellata AR4	876269.3	wgs	patric	876269
66792	Methyloferula stellata AR4	2516653063	draft	img	876269

Genome-based predictions

predictions

trait	prediction	confidence	training_data
motile	yes	78.724	no
flagellated	no	74.541	no
gram-positive	no	97.773	no
anaerobic	no	93.458	no
halophile	no	94.012	no
spore-forming	no	93.824	no
thermophile	no	97.736	no
glucose-util	yes	76.452	no
aerobic	yes	70.891	no
glucose-ferment	no	93.462	no

External links

@ref: 16139

culture collection no.: DSM 22108, LMG 25277, VKM B-2543

straininfo link

@ref: 71323
straininfo: 378352

literature

topic: Phylogeny
Pubmed-ID: 21097638
title: Methyloferula stellata gen. nov., sp. nov., an acidophilic, obligately methanotrophic bacterium that possesses only a soluble methane monooxygenase.
authors: Vorobev AV, Baani M, Doronina NV, Brady AL, Liesack W, Dunfield PF, Dedysh SN
journal: Int J Syst Evol Microbiol
DOI: 10.1099/ijs.0.028118-0
year: 2010
mesh: Bacterial Proteins/metabolism, Base Composition, Beijerinckiaceae/*classification/genetics/*isolation & purification/physiology, Carbon/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Environmental Microbiology, Fatty Acids/analysis, Gene Expression Profiling, Germany, Hydrogen-Ion Concentration, Methane/metabolism, Methanol/metabolism, Molecular Sequence Data, Nitrogen Fixation, Oxygenases/*metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Russia, Sequence Analysis, DNA, Temperature
topic2: Metabolism

Reference

@id	authors	catalogue	doi/url	title
16139	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22108)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-22108
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
71323	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID378352.1	StrainInfo: A central database for resolving microbial strain identifiers