Strain identifier

BacDive ID: 16748

Type strain: Yes

Species: Laceyella sediminis

Strain Designation: RHA1

Strain history: <- L.-B. Lin, YIM; RHA1

NCBI tax ID(s): 573074 (species)

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General

@ref: 16035

BacDive-ID: 16748

DSM-Number: 45263

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, thermophilic, Gram-positive

description: Laceyella sediminis RHA1 is an aerobe, spore-forming, thermophilic bacterium that was isolated from sediment sample of a hot spring .

NCBI tax id

  • NCBI tax id: 573074
  • Matching level: species

strain history

  • @ref: 16035
  • history: <- L.-B. Lin, YIM; RHA1

doi: 10.13145/bacdive16748.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Thermoactinomycetaceae
  • genus: Laceyella
  • species: Laceyella sediminis
  • full scientific name: Laceyella sediminis Chen et al. 2012

@ref: 16035

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Thermoactinomycetaceae

genus: Laceyella

species: Laceyella sediminis

full scientific name: Laceyella sediminis Chen et al. 2012

strain designation: RHA1

type strain: yes

Morphology

cell morphology

  • @ref: 30013
  • gram stain: positive
  • motility: no

pigmentation

  • @ref: 30013
  • production: no

multimedia

  • @ref: 16035
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_45263.jpg
  • caption: Medium 830 55°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16035R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
16035SULFOLOBUS MEDIUM (DSMZ Medium 88)yeshttps://mediadive.dsmz.de/medium/88Name: SULFOLOBUS MEDIUM (DSMZ Medium 88) Composition: (NH4)2SO4 1.28713 g/l Yeast extract 0.990099 g/l KH2PO4 0.277228 g/l MgSO4 x 7 H2O 0.247525 g/l CaCl2 x 2 H2O 0.0693069 g/l FeCl3 x 6 H2O 0.019802 g/l Na2B4O7 x 10 H2O 0.00445545 g/l MnCl2 x 4 H2O 0.00178218 g/l ZnSO4 x 7 H2O 0.000217822 g/l CuCl2 x 2 H2O 4.95049e-05 g/l Na2MoO4 x 2 H2O 2.9703e-05 g/l VOSO4 x 2 H2O 2.9703e-05 g/l CoSO4 x 7 H2O 9.90099e-06 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16035positivegrowth55thermophilic
30013positivegrowth28-65
30013positiveoptimum55thermophilic

culture pH

@refabilitytypepHPH range
30013positivegrowth5.0-9.0alkaliphile
30013positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 30013
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30013
  • spore formation: yes

halophily

  • @ref: 30013
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-1 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
300135291gelatin+carbon source
3001317716lactose+carbon source
3001317306maltose+carbon source
3001327082trehalose+carbon source

enzymes

  • @ref: 30013
  • value: gelatinase
  • activity: +

Isolation, sampling and environmental information

isolation

  • @ref: 16035
  • sample type: sediment sample of a hot spring (55 °C, pH 6.5)
  • geographic location: Yunnan Province, Tengchong county
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_11727.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_284;96_5917;97_7121;98_8795;99_11727&stattab=map
  • Last taxonomy: Laceyella
  • 16S sequence: FJ422144
  • Sequence Identity:
  • Total samples: 13670
  • soil counts: 7909
  • aquatic counts: 1320
  • animal counts: 2143
  • plant counts: 2298

Safety information

risk assessment

  • @ref: 16035
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16035
  • description: Laceyella sediminis strain RHA1 16S ribosomal RNA gene, partial sequence
  • accession: FJ422144
  • length: 1428
  • database: ena
  • NCBI tax ID: 573074

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Laceyella sediminis RHA1GCA_003003125contigncbi573074
66792Laceyella sediminis DSM 452632728369511draftimg573074
66792Laceyella sediminis CCTCC AA 2080582784132168draftimg573074

GC content

@refGC-contentmethod
1603547.9high performance liquid chromatography (HPLC)
3001347.9

External links

@ref: 16035

culture collection no.: DSM 45263, CCTCC AA 208058

straininfo link

  • @ref: 85766
  • straininfo: 400973

literature

  • topic: Phylogeny
  • Pubmed-ID: 21317276
  • title: Laceyella sediminis sp. nov., a thermophilic bacterium isolated from a hot spring.
  • authors: Chen JJ, Lin LB, Zhang LL, Zhang J, Tang SK, Wei YL, Li WJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.028282-0
  • year: 2011
  • mesh: Bacillales/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Glucose/analysis, Hot Springs/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Ribose/analysis, Sequence Analysis, DNA, Spores, Bacterial/cytology, Vitamin K 2/analysis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
16035Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45263)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45263
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30013Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2637728776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
85766Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID400973.1StrainInfo: A central database for resolving microbial strain identifiers