Strain identifier
BacDive ID: 16691
Type strain: 
Species: Thermohydrogenium kirishiense
Strain Designation: ZE-7
Strain history: <- T. Mitrofanova, ZE-7
NCBI tax ID(s): 937254 (species)
version 8.1 (current version)
General
@ref: 4174
BacDive-ID: 16691
DSM-Number: 11055
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, motile
description: Thermohydrogenium kirishiense ZE-7 is an anaerobe, spore-forming, thermophilic bacterium that was isolated from industrial yeast biomass.
NCBI tax id
- NCBI tax id: 937254
- Matching level: species
strain history
- @ref: 4174
- history: <- T. Mitrofanova, ZE-7
doi: 10.13145/bacdive16691.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Syntrophomonadaceae
- genus: Thermohydrogenium
- species: Thermohydrogenium kirishiense
- full scientific name: Thermohydrogenium kirishiense Zacharova et al. 1996
@ref: 4174
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Syntrophomonadaceae
genus: Thermohydrogenium
species: Thermohydrogenium kirishiense
full scientific name: Thermohydrogenium kirishiense Zacharova et al. 1996
strain designation: ZE-7
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 91.651 | |
| 69480 | 100 | positive |
Culture and growth conditions
culture medium
- @ref: 4174
- name: CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640)
- growth: yes
- link: https://mediadive.dsmz.de/medium/640
- composition: Name: CALDICELLULOSIRUPTOR MEDIUM (DSMZ Medium 640; with strain-specific modifications) Composition: D-Glucose 5.0 g/l Trypticase peptone 2.0 g/l K2HPO4 1.5 g/l Yeast extract 1.0 g/l NH4Cl 0.9 g/l NaCl 0.9 g/l KH2PO4 0.75 g/l L-Cysteine HCl x H2O 0.75 g/l MgCl2 x 6 H2O 0.4 g/l HCl 0.0025 g/l FeCl3 x 6 H2O 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Sodium resazurin 0.0005 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 4174 | positive | growth | 60 | thermophilic | |
| 69480 | thermophilic | 99.999 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 4174 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 98 |
| 69480 | yes | 99.494 |
Isolation, sampling and environmental information
isolation
- @ref: 4174
- sample type: industrial yeast biomass
- country: Russia
- origin.country: RUS
isolation source categories
| Cat1 | Cat2 |
|---|---|
| #Engineered | #Industrial |
| #Host | #Yeast |
taxonmaps
- @ref: 69479
- File name: preview.99_4787.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15961;96_838;97_980;98_3585;99_4787&stattab=map
- Last taxonomy: Clostridia
- 16S sequence: FR749965
- Sequence Identity:
- Total samples: 1743
- soil counts: 73
- aquatic counts: 222
- animal counts: 1426
- plant counts: 22
Safety information
risk assessment
- @ref: 4174
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 4174 | Thermohydrogenium kirishiense partial 16S rRNA gene, type strain DSM 11055T, clone 1 | FR749963 | 1579 | ena | 937254 |
| 4174 | Thermohydrogenium kirishiense partial 16S rRNA gene, type strain DSM 11055T, clone 2 | FR749964 | 1506 | ena | 937254 |
| 4174 | Thermohydrogenium kirishiense partial 16S rRNA gene, type strain DSM 11055T, clone 3 | FR749965 | 1508 | ena | 937254 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermohydrogenium kirishiense strain DSM 11055 | 937254.3 | wgs | patric | 937254 |
| 66792 | Thermohydrogenium kirishiense DSM 11055 | 2795386141 | draft | img | 937254 |
GC content
- @ref: 4174
- GC-content: 33.2
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 98 | no |
| motile | yes | 84.947 | no |
| flagellated | no | 84.162 | no |
| gram-positive | no | 76.05 | no |
| anaerobic | yes | 99.576 | no |
| halophile | no | 97.498 | no |
| spore-forming | yes | 95.085 | no |
| glucose-util | yes | 91.233 | no |
| aerobic | no | 99.037 | yes |
| thermophile | yes | 99.723 | yes |
| glucose-ferment | yes | 65.141 | no |
External links
@ref: 4174
culture collection no.: DSM 11055
straininfo link
- @ref: 85710
- straininfo: 49008
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 4174 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11055) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-11055 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
| 85710 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49008.1 | StrainInfo: A central database for resolving microbial strain identifiers |