Strain identifier

BacDive ID: 16468

Type strain: Yes

Species: Streptomyces ascomycinicus

Strain Designation: A-9814, BL-504, KK 317, MA 6475, KK317

Strain history: CIP <- 2000, IFO <- DSMZ <- ATCC <- Bristol Lab. <- T. Arai: strain KK317

NCBI tax ID(s): 476551 (species)

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General

@ref: 9825

BacDive-ID: 16468

DSM-Number: 40822

keywords: 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped, antibiotic compound production

description: Streptomyces ascomycinicus A-9814 is an obligate aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 476551
  • Matching level: species

strain history

@refhistory
9825<- H.D. Tresner, BL-504 <- T. Arai; KK 317 (A-9814)
67770KCC S-0964 <-- DSM 40822 <-- Bristol Labs.; BL-504 <-- T. Arai KK 317.
120018CIP <- 2000, IFO <- DSMZ <- ATCC <- Bristol Lab. <- T. Arai: strain KK317

doi: 10.13145/bacdive16468.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces ascomycinicus
  • full scientific name: Streptomyces ascomycinicus Kumar and Goodfellow 2010

@ref: 9825

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces ascomycinicus

full scientific name: Streptomyces ascomycinicus Kumar and Goodfellow 2010

strain designation: A-9814, BL-504, KK 317, MA 6475, KK317

type strain: yes

Morphology

cell morphology

  • @ref: 120018
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium usedhemolysis ability
18676Golden yellow (1004)10-14 daysISP 2
18676Sand yellow (1002)10-14 daysISP 3
18676Beige (1001)10-14 daysISP 4
18676Golden yellow (1004)10-14 daysISP 5
18676Ivory (1014)10-14 daysISP 6
18676Nut brown (8011)10-14 daysISP 7
1200181

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
18676yesAerial MyceliumDusty grey (7037)ISP 2
18676yesAerial MyceliumBlue grey (7031)ISP 3
18676yesAerial MyceliumDusty grey (7037)ISP 4
18676noISP 5
18676noISP 6
18676noISP 7

pigmentation

  • @ref: 29391
  • production: yes

multimedia

  • @ref: 9825
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40822.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9825GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
9825ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf
18676ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18676ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18676ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18676ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18676ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18676ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
35997MEDIUM 128 - for Nocardia brevicatena, Nocardia flavorosea subsp. fusca and Streptomyces ambofaciensyesDistilled water make up to (1000.000 ml);Maltose (10.000 g);Agar (20.000g);Yeast extract (1.000 g);Beef extract (1.000 g);Casamino acids (2.000 g)
120018CIP Medium 129yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=129

culture temp

@refgrowthtypetemperaturerange
9825positivegrowth28mesophilic
18676positiveoptimum28mesophilic
29391positivegrowth10-37
35997positivegrowth30mesophilic
67770positivegrowth28mesophilic
120018positivegrowth25-37mesophilic
120018nogrowth10psychrophilic
120018nogrowth41thermophilic
120018nogrowth45thermophilic

culture pH

  • @ref: 29391
  • ability: positive
  • type: growth
  • pH: 04-10
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 120018
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 29391
  • spore formation: yes

compound production

@refcompound
9825ascomycin
18676Ascomycin

halophily

@refsaltgrowthtested relationconcentration
29391NaClpositivegrowth10 %
120018NaClpositivegrowth0-2 %
120018NaClnogrowth6 %
120018NaClnogrowth8 %
120018NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1867617234glucose+
1867622599arabinose+
1867617992sucrose-
1867618222xylose-
1867617268myo-inositol-
1867629864mannitol+
1867628757fructose+
1867626546rhamnose+
1867616634raffinose-
1867662968cellulose-
2939122599arabinose+carbon source
2939118403L-arabitol+carbon source
2939117057cellobiose+carbon source
2939123652dextrin+carbon source
2939128757fructose+carbon source
2939133984fucose+carbon source
2939128260galactose+carbon source
2939129987glutamate+carbon source
2939128087glycogen+carbon source
2939117716lactose+carbon source
2939117268myo-inositol+carbon source
2939126271proline+carbon source
2939116634raffinose+carbon source
2939126546rhamnose+carbon source
2939133942ribose+carbon source
2939117814salicin+carbon source
2939118222xylose+carbon source
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin+builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
12001816947citrate-carbon source
1200184853esculin+hydrolysis
120018606565hippurate-hydrolysis
12001817632nitrate-reduction
12001816301nitrite-reduction
12001817632nitrate-respiration

antibiotic resistance

  • @ref: 120018
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6777029582ascomycinyes
12001835581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12001815688acetoin-
12001817234glucose-

enzymes

@refvalueactivityec
120018oxidase-
120018beta-galactosidase+3.2.1.23
120018alcohol dehydrogenase-1.1.1.1
120018gelatinase+
120018amylase+
120018DNase-
120018caseinase+3.4.21.50
120018catalase+1.11.1.6
120018tween esterase-
120018gamma-glutamyltransferase+2.3.2.2
120018lecithinase-
120018lipase+
120018lysine decarboxylase-4.1.1.18
120018ornithine decarboxylase-4.1.1.17
120018phenylalanine ammonia-lyase-4.3.1.24
120018protease+
120018tryptophan deaminase-
120018urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18676+---++++++++--++++-
120018+++-+-+++++-----++--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120018----------+-------------+------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120018++--+----+-----++-++++--+++----+++---+--++-+++--++++--++-+-+++++++++-+-+++++--+++------+++++-++++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
9825soilKobe City, SannomiyaJapanJPNAsia
67770SoilSannomiya, Kobe CityJapanJPNAsia
120018Environment, SoilKobeJapanJPNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
98251Risk group (German classification)
1200181Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces hygroscopicus gene for 16S rRNA, partial sequence, strain:ATCC 14891AB2176031475ena1912
9825Streptomyces ascomycinicus strain DSM 40822 16S ribosomal RNA gene, partial sequenceEU1701211451ena476551

GC content

  • @ref: 9825
  • GC-content: 70.2

External links

@ref: 9825

culture collection no.: DSM 40822, ATCC 14891, CGMCC AS 4.1388, IFO 13981, JCM 4964, KCC S-0964, NBRC 13981, BCRC 11823, CECT 3139, CIP 106837, IFM 1253, HUT 6598

straininfo link

  • @ref: 85504
  • straininfo: 92749

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19656929Reclassification of Streptomyces hygroscopicus strains as Streptomyces aldersoniae sp. nov., Streptomyces angustmyceticus sp. nov., comb. nov., Streptomyces ascomycinicus sp. nov., Streptomyces decoyicus sp. nov., comb. nov., Streptomyces milbemycinicus sp. nov. and Streptomyces wellingtoniae sp. nov.Kumar Y, Goodfellow MInt J Syst Evol Microbiol10.1099/ijs.0.012161-02009Bacterial Typing Techniques, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis, Genes, rRNA, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Soil Microbiology, Species Specificity, Streptomyces/*classification/genetics/metabolism/physiologyGenetics
Genetics27800124Draft genome sequence of Streptomyces sp. TP-A0867, an alchivemycin producer.Komaki H, Ichikawa N, Oguchi A, Hamada M, Harunari E, Kodani S, Fujita N, Igarashi YStand Genomic Sci10.1186/s40793-016-0207-12016

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
9825Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40822)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40822
18676Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40822.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
29391Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604125798
35997Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19012
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
85504Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92749.1StrainInfo: A central database for resolving microbial strain identifiers
120018Curators of the CIPCollection of Institut Pasteur (CIP 106837)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106837