Strain identifier

BacDive ID: 163942

Type strain: No

Species: Acinetobacter mesopotamicus

Strain Designation: GC2

Strain history: <- K. Güven; <- O.Acer, Faculty Sci., Dicle Univ., Diyarbakir, Turkey; GC2

NCBI tax ID(s): 28090 (species)

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General

@ref: 67770

BacDive-ID: 163942

DSM-Number: 26953

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, coccus-shaped, colony-forming

description: Acinetobacter mesopotamicus GC2 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from Oil-contaminated soil in Bozku? 1 petroleum station in Diyarbakir in the southeast of Turkey.

NCBI tax id

  • NCBI tax id: 28090
  • Matching level: species

strain history

@refhistory
67770K. Guven; Dicle Univ., Turkey; GC2.
68383<- K. Güven; <- O.Acer, Faculty Sci., Dicle Univ., Diyarbakir, Turkey; GC2

doi: 10.13145/bacdive163942.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter mesopotamicus
  • full scientific name: Acinetobacter mesopotamicus Acer et al. 2020

@ref: 67770

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter lwoffii

full scientific name: Acinetobacter mesopotamicus Acer et al.

strain designation: GC2

type strain: no

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
68311negativecoccus-shapedno
125438negative99

colony morphology

  • @ref: 68311
  • colony shape: circular
  • incubation period: 2 days
  • medium used: NB-agar

Culture and growth conditions

culture medium

@refnamegrowthlink
68383CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium220.pdf
68383TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium535.pdf

culture temp

@refgrowthtypetemperature
68383positivegrowth28
67770positivegrowth30
68311positivegrowth10-35
68311positiveoptimum25-30

culture pH

@refabilitytypepHPH range
68311positivegrowth6-11alkaliphile
68311positiveoptimum6.5-10

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
68311obligate aerobe
125438aerobe90.814
125439obligate aerobe98.6

spore formation

@refspore formationconfidence
68311no
125438no94.653

halophily

  • @ref: 68311
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-3 %(w/v)

observation

@refobservation
67770quinones: Q-8
68311growth in crude oil and long-chain hydrocarbons

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6831128017starch+assimilation
6831116199urea+assimilation
6831116947citrate-assimilation
683115291gelatin-hydrolysis
6831118186tyrosine-assimilation
6831117634D-glucose+carbon source
6831117716lactose+carbon source
6831130849L-arabinose+carbon source
6831165327D-xylose+carbon source
6831117634D-glucose+energy source
6831117716lactose+energy source
6831130849L-arabinose+energy source
6831165327D-xylose+energy source
6831117306maltose-assimilation
6831128260galactose-assimilation
6831115824D-fructose-assimilation
6831117992sucrose-assimilation
6831127082trehalose-assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
6831135581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68311catalase+1.11.1.6
68311cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    68311C14:01.96
    68311C15:0 iso3.52
    68311C15:0 anteiso1.41
    68311C16:120.38
    68311C16:036.51
    68311C18:02.11
    68311C18:1 w9c32.49
  • type of FA analysis: whole cell analysis
  • incubation medium: NB
  • incubation temperature: 30
  • incubation time: 1
  • system: MIS MIDI
  • instrument: Hewlett Packard 5890A gas chromatograph
  • cutoff value:

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
67770---------------+++/---

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture temperature
67770Oil-contaminated soil in Bozku? 1 petroleum station in Diyarbakir in the southeast of Turkey
68311Bozkus 1 petroleum stationDiyarbakirTurkeyTURAsiaBSMsupplemented with 1% crude oil30
68383petroleum-contaminated soilDiyarbakir, Bozku? 1 petroleum stationTurkeyTURAsia

taxonmaps

  • @ref: 69479
  • File name: preview.99_43.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8;96_11;97_38;98_41;99_43&stattab=map
  • Last taxonomy: Acinetobacter
  • 16S sequence: KJ867435
  • Sequence Identity:
  • Total samples: 10947
  • soil counts: 822
  • aquatic counts: 2946
  • animal counts: 6831
  • plant counts: 348

Safety information

risk assessment

  • @ref: 68383
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 68311
  • description: Acinetobacter lwoffii strain GC2 16S ribosomal RNA gene, partial sequence
  • accession: KJ867435
  • length: 1515
  • database: nuccore
  • NCBI tax ID: 28090

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter sp. GC21538357.4wgspatric28090
68311Acinetobacter lwoffii GC2GCA_011058205scaffoldncbi28090

GC content

  • @ref: 68311
  • GC-content: 42.9
  • method: genome sequence analysis

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingno99yes
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.299yes
125438spore-formingspore-formingAbility to form endo- or exosporesno94.653yes
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes90.814no
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno99.475yes
125438motile2+flagellatedAbility to perform flagellated movementno80.166yes
125439BacteriaNetspore_formationAbility to form endo- or exosporesno88.8
125439BacteriaNetmotilityAbility to perform movementno51.6
125439BacteriaNetgram_stainReaction to gram-stainingnegative68.7
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthobligate aerobe98.6

External links

@ref: 67770

culture collection no.: JCM 31073, DSM 26953

straininfo link

  • @ref: 114145
  • straininfo: 398392

literature

  • topic: Phylogeny
  • Pubmed-ID: 32725341
  • title: Acinetobacter mesopotamicus sp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarbakir, in the Southeast of Turkey.
  • authors: Acer O, Guven K, Poli A, Di Donato P, Leone L, Buono L, Guven RG, Nicolaus B, Finore I
  • journal: Curr Microbiol
  • DOI: 10.1007/s00284-020-02134-9
  • year: 2020
  • mesh: *Acinetobacter/genetics, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Nucleic Acid Hybridization, *Petroleum, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, Soil Microbiology, Turkey
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68311Ömer Acer, Kemal Güven, Annarita Poli, Paola Di Donato, Luigi Leone, Lorena Buono, Reyhan Gül Güven, Barbara Nicolaus & Ilaria FinoreAcinetobacter mesopotamicus sp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarbakir, in the Southeast of Turkey10.1007/s00284-020-02134-9Curr Microbiol 77: 3192-3200 2020
68369Automatically annotated from API 20NE
68383Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26953Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26953)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
114145Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID398392.1
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg OvermannPredicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets10.1101/2024.08.12.607695
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardydeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1https://github.com/GenomeNet/deepG