Strain identifier
BacDive ID: 163942
Type strain:
Species: Acinetobacter mesopotamicus
Strain Designation: GC2
Strain history: <- K. Güven; <- O.Acer, Faculty Sci., Dicle Univ., Diyarbakir, Turkey; GC2
NCBI tax ID(s): 28090 (species)
General
@ref: 67770
BacDive-ID: 163942
DSM-Number: 26953
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, coccus-shaped, colony-forming
description: Acinetobacter mesopotamicus GC2 is an obligate aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from Oil-contaminated soil in Bozku? 1 petroleum station in Diyarbakir in the southeast of Turkey.
NCBI tax id
- NCBI tax id: 28090
- Matching level: species
strain history
@ref | history |
---|---|
67770 | K. Guven; Dicle Univ., Turkey; GC2. |
68383 | <- K. Güven; <- O.Acer, Faculty Sci., Dicle Univ., Diyarbakir, Turkey; GC2 |
doi: 10.13145/bacdive163942.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Moraxellaceae
- genus: Acinetobacter
- species: Acinetobacter mesopotamicus
- full scientific name: Acinetobacter mesopotamicus Acer et al. 2020
@ref: 67770
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Moraxellaceae
genus: Acinetobacter
species: Acinetobacter lwoffii
full scientific name: Acinetobacter mesopotamicus Acer et al.
strain designation: GC2
type strain: no
Morphology
cell morphology
@ref | gram stain | cell shape | motility | confidence |
---|---|---|---|---|
68311 | negative | coccus-shaped | no | |
69480 | negative | 99.987 | ||
69480 | no | 93.644 |
colony morphology
- @ref: 68311
- colony shape: circular
- incubation period: 2 days
- medium used: NB-agar
Culture and growth conditions
culture medium
@ref | name | growth | link |
---|---|---|---|
68383 | CASO AGAR (MERCK 105458) (DSMZ Medium 220) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium220.pdf |
68383 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium535.pdf |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
68383 | positive | growth | 28 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
68311 | positive | growth | 10-35 | |
68311 | positive | optimum | 25-30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
68311 | positive | growth | 6-11 | alkaliphile |
68311 | positive | optimum | 6.5-10 |
Physiology and metabolism
oxygen tolerance
- @ref: 68311
- oxygen tolerance: obligate aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
68311 | no | |
69480 | no | 99.994 |
69481 | no | 100 |
halophily
- @ref: 68311
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-3 %(w/v)
observation
@ref | observation |
---|---|
67770 | quinones: Q-8 |
68311 | growth in crude oil and long-chain hydrocarbons |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 17128 | adipate | + | assimilation |
68369 | 27689 | decanoate | + | assimilation |
68369 | 24265 | gluconate | - | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | - | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
68311 | 28017 | starch | + | assimilation |
68311 | 16199 | urea | + | assimilation |
68311 | 16947 | citrate | - | assimilation |
68311 | 5291 | gelatin | - | hydrolysis |
68311 | 18186 | tyrosine | - | assimilation |
68311 | 17634 | D-glucose | + | carbon source |
68311 | 17716 | lactose | + | carbon source |
68311 | 30849 | L-arabinose | + | carbon source |
68311 | 65327 | D-xylose | + | carbon source |
68311 | 17634 | D-glucose | + | energy source |
68311 | 17716 | lactose | + | energy source |
68311 | 30849 | L-arabinose | + | energy source |
68311 | 65327 | D-xylose | + | energy source |
68311 | 17306 | maltose | - | assimilation |
68311 | 28260 | galactose | - | assimilation |
68311 | 15824 | D-fructose | - | assimilation |
68311 | 17992 | sucrose | - | assimilation |
68311 | 27082 | trehalose | - | assimilation |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
68311 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68311 | catalase | + | 1.11.1.6 |
68311 | cytochrome oxidase | - | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
fatty acid profile
fatty acids
@ref fatty acid percentage 68311 C14:0 1.96 68311 C15:0 iso 3.52 68311 C15:0 anteiso 1.41 68311 C16:1 20.38 68311 C16:0 36.51 68311 C18:0 2.11 68311 C18:1 w9c 32.49 - type of FA analysis: whole cell analysis
- incubation medium: NB
- incubation temperature: 30
- incubation time: 1
- system: MIS MIDI
- instrument: Hewlett Packard 5890A gas chromatograph
- cutoff value:
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
67770 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | +/- | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture composition | enrichment culture temperature |
---|---|---|---|---|---|---|---|---|
67770 | Oil-contaminated soil in Bozku? 1 petroleum station in Diyarbakir in the southeast of Turkey | |||||||
68311 | Bozkus 1 petroleum station | Diyarbakir | Turkey | TUR | Asia | BSM | supplemented with 1% crude oil | 30 |
taxonmaps
- @ref: 69479
- File name: preview.99_43.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_8;96_11;97_38;98_41;99_43&stattab=map
- Last taxonomy: Acinetobacter
- 16S sequence: KJ867435
- Sequence Identity:
- Total samples: 10947
- soil counts: 822
- aquatic counts: 2946
- animal counts: 6831
- plant counts: 348
Safety information
risk assessment
- @ref: 68383
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 68311
- description: Acinetobacter mesopotamicus strain GC2 16S ribosomal RNA gene, partial sequence
- accession: KJ867435
- length: 1515
- database: ena
- NCBI tax ID: 28090
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Acinetobacter sp. GC2 | 1538357.4 | wgs | patric | 28090 |
68311 | Acinetobacter lwoffii GC2 | GCA_011058205 | scaffold | ncbi | 28090 |
GC content
- @ref: 68311
- GC-content: 42.9
- method: genome sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 92.55 | no |
gram-positive | no | 97.421 | yes |
anaerobic | no | 98.763 | yes |
aerobic | yes | 90.493 | yes |
halophile | no | 80.846 | no |
spore-forming | no | 96.911 | yes |
thermophile | no | 99.856 | no |
glucose-util | no | 87.258 | no |
flagellated | no | 95.885 | yes |
glucose-ferment | no | 92.291 | yes |
External links
@ref: 67770
culture collection no.: JCM 31073, DSM 26953
straininfo link
- @ref: 114145
- straininfo: 398392
literature
- topic: Phylogeny
- Pubmed-ID: 32725341
- title: Acinetobacter mesopotamicus sp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarbakir, in the Southeast of Turkey.
- authors: Acer O, Guven K, Poli A, Di Donato P, Leone L, Buono L, Guven RG, Nicolaus B, Finore I
- journal: Curr Microbiol
- DOI: 10.1007/s00284-020-02134-9
- year: 2020
- mesh: *Acinetobacter/genetics, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/analysis, Nucleic Acid Hybridization, *Petroleum, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil, Soil Microbiology, Turkey
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | catalogue |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||
68311 | Ömer Acer, Kemal Güven, Annarita Poli, Paola Di Donato, Luigi Leone, Lorena Buono, Reyhan Gül Güven, Barbara Nicolaus & Ilaria Finore | Acinetobacter mesopotamicus sp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarbakir, in the Southeast of Turkey | 10.1007/s00284-020-02134-9 | Curr Microbiol 77: 3192-3200 2020 | |
68369 | Automatically annotated from API 20NE | ||||
68383 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-26953 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26953) | ||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | ||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | ||
114145 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID398392.1 |