Strain identifier
BacDive ID: 16385
Type strain: 
Species: Streptantibioticus ferralitis
Strain Designation: SF0p68
Strain history: NCIMB 13954 <-- M. Goodfellow SFOp68.
NCBI tax ID(s): 236510 (species)
version 8.1 (current version)
General
@ref: 10619
BacDive-ID: 16385
DSM-Number: 41836
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive
description: Streptantibioticus ferralitis SF0p68 is an aerobe, spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from ferralitic oxydic soil sample.
NCBI tax id
- NCBI tax id: 236510
- Matching level: species
strain history
| @ref | history |
|---|---|
| 10619 | <- M. Goodfellow; SF0p68 <- D. Saintpierre-Bonaccio, Univ. Newcastle |
| 67770 | NCIMB 13954 <-- M. Goodfellow SFOp68. |
doi: 10.13145/bacdive16385.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptantibioticus
- species: Streptantibioticus ferralitis
- full scientific name: Streptantibioticus ferralitis (Saintpierre-Bonaccio et al. 2004) ?wiecimska et al. 2023
synonyms
- @ref: 20215
- synonym: Streptomyces ferralitis
@ref: 10619
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces ferralitis
full scientific name: Streptomyces ferralitis Saintpierre-Bonaccio et al. 2004
strain designation: SF0p68
type strain: yes
Morphology
cell morphology
- @ref: 30057
- gram stain: positive
colony morphology
| @ref | colony color | incubation period | medium used |
|---|---|---|---|
| 19532 | Beige | 10-14 days | ISP 2 |
| 19532 | Beige | 10-14 days | ISP 3 |
| 19532 | Beige | 10-14 days | ISP 4 |
| 19532 | Beige | 10-14 days | ISP 5 |
| 19532 | Beige | 10-14 days | ISP 6 |
| 19532 | Beige | 10-14 days | ISP 7 |
multicellular morphology
| @ref | forms multicellular complex | complex name | complex color | medium name |
|---|---|---|---|---|
| 19532 | yes | Aerial Mycelium | White | ISP 2 |
| 19532 | no | ISP 3 | ||
| 19532 | no | ISP 4 | ||
| 19532 | no | ISP 5 | ||
| 19532 | no | ISP 6 | ||
| 19532 | no | ISP 7 |
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 10619 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 19532 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
| 19532 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
| 19532 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
| 19532 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
| 19532 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 19532 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
| 10619 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://mediadive.dsmz.de/medium/84 | Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water |
| 10619 | BENNETT'S AGAR (DSMZ Medium 548) | yes | https://mediadive.dsmz.de/medium/548 | Name: BENNETT'S AGAR (DSMZ Medium 548) Composition: Agar 15.0 g/l Glucose 10.0 g/l N-Z amine 2.0 g/l Yeast extract 1.0 g/l Beef extract 1.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 10619 | positive | growth | 28 | mesophilic |
| 19532 | positive | optimum | 28 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 30057
- oxygen tolerance: aerobe
spore formation
- @ref: 30057
- spore formation: yes
observation
- @ref: 67770
- observation: quinones: MK-9(H8)
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 19532 | 17234 | glucose | + | |
| 19532 | 22599 | arabinose | - | |
| 19532 | 17992 | sucrose | - | |
| 19532 | 18222 | xylose | - | |
| 19532 | 17268 | myo-inositol | + | |
| 19532 | 29864 | mannitol | + | |
| 19532 | 28757 | fructose | - | |
| 19532 | 26546 | rhamnose | - | |
| 19532 | 16634 | raffinose | - | |
| 19532 | 62968 | cellulose | - | |
| 30057 | 28260 | galactose | + | carbon source |
| 30057 | 17234 | glucose | + | carbon source |
| 30057 | 29864 | mannitol | + | carbon source |
| 30057 | 37684 | mannose | + | carbon source |
| 30057 | 27082 | trehalose | + | carbon source |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 30057 | urease | + | 3.5.1.5 |
| 68382 | alkaline phosphatase | + | 3.1.3.1 |
| 68382 | esterase (C 4) | + | |
| 68382 | esterase lipase (C 8) | + | |
| 68382 | lipase (C 14) | - | |
| 68382 | leucine arylamidase | + | 3.4.11.1 |
| 68382 | valine arylamidase | + | |
| 68382 | cystine arylamidase | - | 3.4.11.3 |
| 68382 | trypsin | - | 3.4.21.4 |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | |
| 68382 | alpha-galactosidase | + | 3.2.1.22 |
| 68382 | beta-galactosidase | + | 3.2.1.23 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | alpha-glucosidase | + | 3.2.1.20 |
| 68382 | beta-glucosidase | - | 3.2.1.21 |
| 68382 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
| 68382 | alpha-mannosidase | + | 3.2.1.24 |
| 68382 | alpha-fucosidase | + | 3.2.1.51 |
API zym
| @ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 19532 | + | + | + | - | + | + | - | - | - | + | + | + | + | - | + | - | + | + | + |
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent | geographic location |
|---|---|---|---|---|---|
| 10619 | ferralitic oxydic soil sample | New Caledonia | NCL | Australia and Oceania | |
| 67770 | Ferralitic, oxidic, ultramafic soil | French Overseas | FRA | southern part of main island of New Caledonia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 10619 | 1 | Risk group (German classification) |
| 19532 | 1 | Risk group (German classification) |
Sequence information
16S sequences
- @ref: 10619
- description: Streptomyces ferralitis 16S ribosomal RNA gene, partial sequence
- accession: AY262826
- length: 1490
- database: ena
- NCBI tax ID: 236510
Genome sequences
- @ref: 66792
- description: Streptantibioticus ferralitis JCM 14344
- accession: GCA_029223525
- assembly level: contig
- database: ncbi
- NCBI tax ID: 236510
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 92.295 | no |
| flagellated | no | 97.138 | no |
| gram-positive | yes | 78.9 | no |
| anaerobic | no | 95.409 | yes |
| aerobic | yes | 86.889 | yes |
| halophile | no | 77.544 | no |
| spore-forming | no | 79.286 | no |
| glucose-ferment | no | 61.002 | no |
| thermophile | no | 99.694 | yes |
| glucose-util | yes | 89.765 | yes |
External links
@ref: 10619
culture collection no.: DSM 41836, CGMCC 4.1985, NCIMB 13954, JCM 14344, VTT E-072715
straininfo link
- @ref: 85435
- straininfo: 134442
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 15545435 | Streptomyces ferralitis sp. nov., a novel streptomycete isolated from a New-Caledonian ultramafic soil. | Saintpierre-Bonaccio D, Amir H, Pineau R, Lemriss S, Goodfellow M | Int J Syst Evol Microbiol | 10.1099/ijs.0.02891-0 | 2004 | DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Genes, rRNA, Molecular Sequence Data, New Caledonia, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/cytology/genetics/*isolation & purification | Enzymology |
| Phylogeny | 26297487 | Streptomyces rubrisoli sp. nov., neutrotolerant acidophilic actinomycetes isolated from red soil. | Guo X, Zhang L, Li X, Gao Y, Ruan J, Huang Y | Int J Syst Evol Microbiol | 10.1099/ijs.0.000383 | 2015 | Actinobacteria/genetics, Actinomyces/genetics, Actinomycetales/classification, Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride, Soil, Soil Microbiology, *Streptomyces/classification | Genetics |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 10619 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 41836) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-41836 | |||
| 19532 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM41836.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 30057 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26418 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 68382 | Automatically annotated from API zym | |||||
| 85435 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID134442.1 | StrainInfo: A central database for resolving microbial strain identifiers |